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Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis
Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to charact...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140677/ https://www.ncbi.nlm.nih.gov/pubmed/32183400 http://dx.doi.org/10.3390/cells9030721 |
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| author | Lapitz, Ainhoa Arbelaiz, Ander O’Rourke, Colm J. Lavin, Jose L. La Casta, Adelaida Ibarra, Cesar Jimeno, Juan P. Santos-Laso, Alvaro Izquierdo-Sanchez, Laura Krawczyk, Marcin Perugorria, Maria J. Jimenez-Aguero, Raul Sanchez-Campos, Alberto Riaño, Ioana Gónzalez, Esperanza Lammert, Frank Marzioni, Marco Macias, Rocio I.R. Marin, Jose J.G. Karlsen, Tom H. Bujanda, Luis Falcón-Pérez, Juan M. Andersen, Jesper B. Aransay, Ana M. Rodrigues, Pedro M. Banales, Jesus M. |
| author_facet | Lapitz, Ainhoa Arbelaiz, Ander O’Rourke, Colm J. Lavin, Jose L. La Casta, Adelaida Ibarra, Cesar Jimeno, Juan P. Santos-Laso, Alvaro Izquierdo-Sanchez, Laura Krawczyk, Marcin Perugorria, Maria J. Jimenez-Aguero, Raul Sanchez-Campos, Alberto Riaño, Ioana Gónzalez, Esperanza Lammert, Frank Marzioni, Marco Macias, Rocio I.R. Marin, Jose J.G. Karlsen, Tom H. Bujanda, Luis Falcón-Pérez, Juan M. Andersen, Jesper B. Aransay, Ana M. Rodrigues, Pedro M. Banales, Jesus M. |
| author_sort | Lapitz, Ainhoa |
| collection | PubMed |
| description | Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to characterize the transcriptomic profile of serum and urine extracellular vesicles (EVs) from patients with CCA, PSC, ulcerative colitis (UC), and healthy individuals. Serum and urine EVs were isolated by serial ultracentrifugations and characterized by nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting. EVs transcriptome was determined by Illumina gene expression array [messenger RNAs (mRNA) and non-coding RNAs (ncRNAs)]. Differential RNA profiles were found in serum and urine EVs from patients with CCA compared to control groups (disease and healthy), showing high diagnostic capacity. The comparison of the mRNA profiles of serum or urine EVs from patients with CCA with the transcriptome of tumor tissues from two cohorts of patients, CCA cells in vitro, and CCA cells-derived EVs, identified 105 and 39 commonly-altered transcripts, respectively. Gene ontology analysis indicated that most commonly-altered mRNAs participate in carcinogenic steps. Overall, patients with CCA present specific RNA profiles in EVs mirroring the tumor, and constituting novel promising liquid biopsy biomarkers. |
| format | Online Article Text |
| id | pubmed-7140677 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-71406772020-04-13 Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis Lapitz, Ainhoa Arbelaiz, Ander O’Rourke, Colm J. Lavin, Jose L. La Casta, Adelaida Ibarra, Cesar Jimeno, Juan P. Santos-Laso, Alvaro Izquierdo-Sanchez, Laura Krawczyk, Marcin Perugorria, Maria J. Jimenez-Aguero, Raul Sanchez-Campos, Alberto Riaño, Ioana Gónzalez, Esperanza Lammert, Frank Marzioni, Marco Macias, Rocio I.R. Marin, Jose J.G. Karlsen, Tom H. Bujanda, Luis Falcón-Pérez, Juan M. Andersen, Jesper B. Aransay, Ana M. Rodrigues, Pedro M. Banales, Jesus M. Cells Article Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to characterize the transcriptomic profile of serum and urine extracellular vesicles (EVs) from patients with CCA, PSC, ulcerative colitis (UC), and healthy individuals. Serum and urine EVs were isolated by serial ultracentrifugations and characterized by nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting. EVs transcriptome was determined by Illumina gene expression array [messenger RNAs (mRNA) and non-coding RNAs (ncRNAs)]. Differential RNA profiles were found in serum and urine EVs from patients with CCA compared to control groups (disease and healthy), showing high diagnostic capacity. The comparison of the mRNA profiles of serum or urine EVs from patients with CCA with the transcriptome of tumor tissues from two cohorts of patients, CCA cells in vitro, and CCA cells-derived EVs, identified 105 and 39 commonly-altered transcripts, respectively. Gene ontology analysis indicated that most commonly-altered mRNAs participate in carcinogenic steps. Overall, patients with CCA present specific RNA profiles in EVs mirroring the tumor, and constituting novel promising liquid biopsy biomarkers. MDPI 2020-03-14 /pmc/articles/PMC7140677/ /pubmed/32183400 http://dx.doi.org/10.3390/cells9030721 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Lapitz, Ainhoa Arbelaiz, Ander O’Rourke, Colm J. Lavin, Jose L. La Casta, Adelaida Ibarra, Cesar Jimeno, Juan P. Santos-Laso, Alvaro Izquierdo-Sanchez, Laura Krawczyk, Marcin Perugorria, Maria J. Jimenez-Aguero, Raul Sanchez-Campos, Alberto Riaño, Ioana Gónzalez, Esperanza Lammert, Frank Marzioni, Marco Macias, Rocio I.R. Marin, Jose J.G. Karlsen, Tom H. Bujanda, Luis Falcón-Pérez, Juan M. Andersen, Jesper B. Aransay, Ana M. Rodrigues, Pedro M. Banales, Jesus M. Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis |
| title | Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis |
| title_full | Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis |
| title_fullStr | Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis |
| title_full_unstemmed | Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis |
| title_short | Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis |
| title_sort | patients with cholangiocarcinoma present specific rna profiles in serum and urine extracellular vesicles mirroring the tumor expression: novel liquid biopsy biomarkers for disease diagnosis |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140677/ https://www.ncbi.nlm.nih.gov/pubmed/32183400 http://dx.doi.org/10.3390/cells9030721 |
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