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3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells

We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties...

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Autores principales: Bordoni, Matteo, Karabulut, Erdem, Kuzmenko, Volodymyr, Fantini, Valentina, Pansarasa, Orietta, Cereda, Cristina, Gatenholm, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140699/
https://www.ncbi.nlm.nih.gov/pubmed/32168750
http://dx.doi.org/10.3390/cells9030682
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author Bordoni, Matteo
Karabulut, Erdem
Kuzmenko, Volodymyr
Fantini, Valentina
Pansarasa, Orietta
Cereda, Cristina
Gatenholm, Paul
author_facet Bordoni, Matteo
Karabulut, Erdem
Kuzmenko, Volodymyr
Fantini, Valentina
Pansarasa, Orietta
Cereda, Cristina
Gatenholm, Paul
author_sort Bordoni, Matteo
collection PubMed
description We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium–induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases.
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spelling pubmed-71406992020-04-13 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells Bordoni, Matteo Karabulut, Erdem Kuzmenko, Volodymyr Fantini, Valentina Pansarasa, Orietta Cereda, Cristina Gatenholm, Paul Cells Article We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium–induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases. MDPI 2020-03-11 /pmc/articles/PMC7140699/ /pubmed/32168750 http://dx.doi.org/10.3390/cells9030682 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bordoni, Matteo
Karabulut, Erdem
Kuzmenko, Volodymyr
Fantini, Valentina
Pansarasa, Orietta
Cereda, Cristina
Gatenholm, Paul
3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
title 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
title_full 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
title_fullStr 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
title_full_unstemmed 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
title_short 3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
title_sort 3d printed conductive nanocellulose scaffolds for the differentiation of human neuroblastoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140699/
https://www.ncbi.nlm.nih.gov/pubmed/32168750
http://dx.doi.org/10.3390/cells9030682
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