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Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43
Long non-coding RNAs (lncRNAs) have been implicated in fundamental and diverse biological processes, including myogenesis. However, the molecular mechanisms involved in this process remain largely unexplored. This study found that H19 affected the differentiation of porcine satellite cells (PSCs) by...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140797/ https://www.ncbi.nlm.nih.gov/pubmed/32121115 http://dx.doi.org/10.3390/genes11030259 |
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author | Li, Jingxuan Zhao, Wenjuan Li, Qianqian Huang, Ziying Shi, Gaoli Li, Changchun |
author_facet | Li, Jingxuan Zhao, Wenjuan Li, Qianqian Huang, Ziying Shi, Gaoli Li, Changchun |
author_sort | Li, Jingxuan |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) have been implicated in fundamental and diverse biological processes, including myogenesis. However, the molecular mechanisms involved in this process remain largely unexplored. This study found that H19 affected the differentiation of porcine satellite cells (PSCs) by directly binding to the DNA/RNA-binding protein TDP43. Functional analyses showed that TDP43 knockdown decreased PSC differentiation, whereas TDP43 overexpression exerted opposite effects in vitro. Furthermore, rescue experiments demonstrated that TDP43 can rescue the decrease in PSC differentiation caused by H19 knockdown. Mechanistically, H19 may act as a scaffold to recruit TDP43 to the promoters of MYOD and thereby activate the transcription of MYOD, leading to PSC differentiation. In summary, we elucidate the molecular mechanism by which H19 and TDP43 regulate myogenesis. |
format | Online Article Text |
id | pubmed-7140797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71407972020-04-10 Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 Li, Jingxuan Zhao, Wenjuan Li, Qianqian Huang, Ziying Shi, Gaoli Li, Changchun Genes (Basel) Article Long non-coding RNAs (lncRNAs) have been implicated in fundamental and diverse biological processes, including myogenesis. However, the molecular mechanisms involved in this process remain largely unexplored. This study found that H19 affected the differentiation of porcine satellite cells (PSCs) by directly binding to the DNA/RNA-binding protein TDP43. Functional analyses showed that TDP43 knockdown decreased PSC differentiation, whereas TDP43 overexpression exerted opposite effects in vitro. Furthermore, rescue experiments demonstrated that TDP43 can rescue the decrease in PSC differentiation caused by H19 knockdown. Mechanistically, H19 may act as a scaffold to recruit TDP43 to the promoters of MYOD and thereby activate the transcription of MYOD, leading to PSC differentiation. In summary, we elucidate the molecular mechanism by which H19 and TDP43 regulate myogenesis. MDPI 2020-02-28 /pmc/articles/PMC7140797/ /pubmed/32121115 http://dx.doi.org/10.3390/genes11030259 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Jingxuan Zhao, Wenjuan Li, Qianqian Huang, Ziying Shi, Gaoli Li, Changchun Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 |
title | Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 |
title_full | Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 |
title_fullStr | Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 |
title_full_unstemmed | Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 |
title_short | Long Non-Coding RNA H19 Promotes Porcine Satellite Cell Differentiation by Interacting with TDP43 |
title_sort | long non-coding rna h19 promotes porcine satellite cell differentiation by interacting with tdp43 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140797/ https://www.ncbi.nlm.nih.gov/pubmed/32121115 http://dx.doi.org/10.3390/genes11030259 |
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