Cargando…
Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ±...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140838/ https://www.ncbi.nlm.nih.gov/pubmed/32210018 http://dx.doi.org/10.3390/cells9030778 |
_version_ | 1783519080543682560 |
---|---|
author | Daum, Ruben Visser, Dmitri Wild, Constanze Kutuzova, Larysa Schneider, Maria Lorenz, Günter Weiss, Martin Hinderer, Svenja Stock, Ulrich A. Seifert, Martina Schenke-Layland, Katja |
author_facet | Daum, Ruben Visser, Dmitri Wild, Constanze Kutuzova, Larysa Schneider, Maria Lorenz, Günter Weiss, Martin Hinderer, Svenja Stock, Ulrich A. Seifert, Martina Schenke-Layland, Katja |
author_sort | Daum, Ruben |
collection | PubMed |
description | Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer. |
format | Online Article Text |
id | pubmed-7140838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71408382020-04-10 Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture Daum, Ruben Visser, Dmitri Wild, Constanze Kutuzova, Larysa Schneider, Maria Lorenz, Günter Weiss, Martin Hinderer, Svenja Stock, Ulrich A. Seifert, Martina Schenke-Layland, Katja Cells Article Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer. MDPI 2020-03-23 /pmc/articles/PMC7140838/ /pubmed/32210018 http://dx.doi.org/10.3390/cells9030778 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Daum, Ruben Visser, Dmitri Wild, Constanze Kutuzova, Larysa Schneider, Maria Lorenz, Günter Weiss, Martin Hinderer, Svenja Stock, Ulrich A. Seifert, Martina Schenke-Layland, Katja Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
title | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
title_full | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
title_fullStr | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
title_full_unstemmed | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
title_short | Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture |
title_sort | fibronectin adsorption on electrospun synthetic vascular grafts attracts endothelial progenitor cells and promotes endothelialization in dynamic in vitro culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140838/ https://www.ncbi.nlm.nih.gov/pubmed/32210018 http://dx.doi.org/10.3390/cells9030778 |
work_keys_str_mv | AT daumruben fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT visserdmitri fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT wildconstanze fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT kutuzovalarysa fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT schneidermaria fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT lorenzgunter fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT weissmartin fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT hinderersvenja fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT stockulricha fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT seifertmartina fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture AT schenkelaylandkatja fibronectinadsorptiononelectrospunsyntheticvasculargraftsattractsendothelialprogenitorcellsandpromotesendothelializationindynamicinvitroculture |