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Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ±...

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Autores principales: Daum, Ruben, Visser, Dmitri, Wild, Constanze, Kutuzova, Larysa, Schneider, Maria, Lorenz, Günter, Weiss, Martin, Hinderer, Svenja, Stock, Ulrich A., Seifert, Martina, Schenke-Layland, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140838/
https://www.ncbi.nlm.nih.gov/pubmed/32210018
http://dx.doi.org/10.3390/cells9030778
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author Daum, Ruben
Visser, Dmitri
Wild, Constanze
Kutuzova, Larysa
Schneider, Maria
Lorenz, Günter
Weiss, Martin
Hinderer, Svenja
Stock, Ulrich A.
Seifert, Martina
Schenke-Layland, Katja
author_facet Daum, Ruben
Visser, Dmitri
Wild, Constanze
Kutuzova, Larysa
Schneider, Maria
Lorenz, Günter
Weiss, Martin
Hinderer, Svenja
Stock, Ulrich A.
Seifert, Martina
Schenke-Layland, Katja
author_sort Daum, Ruben
collection PubMed
description Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer.
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spelling pubmed-71408382020-04-10 Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture Daum, Ruben Visser, Dmitri Wild, Constanze Kutuzova, Larysa Schneider, Maria Lorenz, Günter Weiss, Martin Hinderer, Svenja Stock, Ulrich A. Seifert, Martina Schenke-Layland, Katja Cells Article Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer. MDPI 2020-03-23 /pmc/articles/PMC7140838/ /pubmed/32210018 http://dx.doi.org/10.3390/cells9030778 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Daum, Ruben
Visser, Dmitri
Wild, Constanze
Kutuzova, Larysa
Schneider, Maria
Lorenz, Günter
Weiss, Martin
Hinderer, Svenja
Stock, Ulrich A.
Seifert, Martina
Schenke-Layland, Katja
Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
title Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
title_full Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
title_fullStr Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
title_full_unstemmed Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
title_short Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
title_sort fibronectin adsorption on electrospun synthetic vascular grafts attracts endothelial progenitor cells and promotes endothelialization in dynamic in vitro culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140838/
https://www.ncbi.nlm.nih.gov/pubmed/32210018
http://dx.doi.org/10.3390/cells9030778
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