Genome-Wide Identification of lncRNAs During Rice Seed Development

Rice seed is a pivotal reproductive organ that directly determines yield and quality. Long non-coding RNAs (lncRNAs) have been recognized as key regulators in plant development, but the roles of lncRNAs in rice seed development remain unclear. In this study, we performed a paired-end RNA sequencing...

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Detalles Bibliográficos
Autores principales: Zhao, Juan, Ajadi, Abolore Adijat, Wang, Yifeng, Tong, Xiaohong, Wang, Huimei, Tang, Liqun, Li, Zhiyong, Shu, Yazhou, Liu, Xixi, Li, Shufan, Wang, Shuang, Liu, Wanning, Zhang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140839/
https://www.ncbi.nlm.nih.gov/pubmed/32110990
http://dx.doi.org/10.3390/genes11030243
Descripción
Sumario:Rice seed is a pivotal reproductive organ that directly determines yield and quality. Long non-coding RNAs (lncRNAs) have been recognized as key regulators in plant development, but the roles of lncRNAs in rice seed development remain unclear. In this study, we performed a paired-end RNA sequencing in samples of rice pistils and seeds at three and seven days after pollination (DAP) respectively. A total of 540 lncRNAs were obtained, among which 482 lncRNAs had significantly different expression patterns during seed development. Results from semi-qPCR conducted on 15 randomly selected differentially expressed lncRNAs suggested high reliability of the transcriptomic data. RNA interference of TCONS_00023703, which is predominantly transcribed in developing seeds, significantly reduced grain length and thousand-grain weight. These results expanded the dataset of lncRNA in rice and enhanced our understanding of the biological functions of lncRNAs in rice seed development

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