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The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression

Uveal melanoma (UM) is the most common primary tumor of the eye diagnosed in adults, associated with a high risk of metastasis and thereby, poor prognosis. Among known risk factors for the development of metastatic disease is the loss of BAP1 expression and chromosome 3 monosomy in the primary tumor...

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Autores principales: Wróblewska, Joanna Patrycja, Lach, Michał Stefan, Ustaszewski, Adam, Kulcenty, Katarzyna, Ibbs, Matthew, Jagiełło, Inga, Suchorska, Wiktoria Maria, Marszałek, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140886/
https://www.ncbi.nlm.nih.gov/pubmed/32131485
http://dx.doi.org/10.3390/genes11030271
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author Wróblewska, Joanna Patrycja
Lach, Michał Stefan
Ustaszewski, Adam
Kulcenty, Katarzyna
Ibbs, Matthew
Jagiełło, Inga
Suchorska, Wiktoria Maria
Marszałek, Andrzej
author_facet Wróblewska, Joanna Patrycja
Lach, Michał Stefan
Ustaszewski, Adam
Kulcenty, Katarzyna
Ibbs, Matthew
Jagiełło, Inga
Suchorska, Wiktoria Maria
Marszałek, Andrzej
author_sort Wróblewska, Joanna Patrycja
collection PubMed
description Uveal melanoma (UM) is the most common primary tumor of the eye diagnosed in adults, associated with a high risk of metastasis and thereby, poor prognosis. Among known risk factors for the development of metastatic disease is the loss of BAP1 expression and chromosome 3 monosomy in the primary tumor. However, the expression levels of specific micro RNAs (miRNA) in tumor tissue may also serve as a valuable marker for determining the risk of metastatic disease in patients with primary uveal melanoma. In our study, we analyzed the miRNA expression data of cases selected from The Cancer Genome Atlas study on uveal melanoma, and determined a panel of 15 miRNAs differentially expressed between patients with primary and metastatic disease. Next, 6 miRNAs were validated on a group of 46 tumor samples from primary and metastatic patients. We have shown, that expression of hsa-miR-592, hsa-miR-346, and hsa-miR-1247 was significantly increased, while hsa-miR-506 and hsa-miR-513c were decreased in the tumors of patients with metastatic disease. Hsa-miR-196b expression did not differ between the two subgroups, however, we showed significant correlation with BAP1 expression. Moreover, hsa-miR-592 also showed correlation with monosomy 3 tumors. Gene ontology analysis revealed involvement of those miRNAs with cellular processes mediating the metastatic process. Our results showed that miRNAs play an important role in the deregulation of several oncogenic pathways in UM and can, thereby, promote metastatic spread to distant organs. Moreover, differentially expressed miRNAs may be used as an interesting biomarker for the assessment of metastatic risk in uveal melanoma patients.
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spelling pubmed-71408862020-04-10 The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression Wróblewska, Joanna Patrycja Lach, Michał Stefan Ustaszewski, Adam Kulcenty, Katarzyna Ibbs, Matthew Jagiełło, Inga Suchorska, Wiktoria Maria Marszałek, Andrzej Genes (Basel) Article Uveal melanoma (UM) is the most common primary tumor of the eye diagnosed in adults, associated with a high risk of metastasis and thereby, poor prognosis. Among known risk factors for the development of metastatic disease is the loss of BAP1 expression and chromosome 3 monosomy in the primary tumor. However, the expression levels of specific micro RNAs (miRNA) in tumor tissue may also serve as a valuable marker for determining the risk of metastatic disease in patients with primary uveal melanoma. In our study, we analyzed the miRNA expression data of cases selected from The Cancer Genome Atlas study on uveal melanoma, and determined a panel of 15 miRNAs differentially expressed between patients with primary and metastatic disease. Next, 6 miRNAs were validated on a group of 46 tumor samples from primary and metastatic patients. We have shown, that expression of hsa-miR-592, hsa-miR-346, and hsa-miR-1247 was significantly increased, while hsa-miR-506 and hsa-miR-513c were decreased in the tumors of patients with metastatic disease. Hsa-miR-196b expression did not differ between the two subgroups, however, we showed significant correlation with BAP1 expression. Moreover, hsa-miR-592 also showed correlation with monosomy 3 tumors. Gene ontology analysis revealed involvement of those miRNAs with cellular processes mediating the metastatic process. Our results showed that miRNAs play an important role in the deregulation of several oncogenic pathways in UM and can, thereby, promote metastatic spread to distant organs. Moreover, differentially expressed miRNAs may be used as an interesting biomarker for the assessment of metastatic risk in uveal melanoma patients. MDPI 2020-03-02 /pmc/articles/PMC7140886/ /pubmed/32131485 http://dx.doi.org/10.3390/genes11030271 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wróblewska, Joanna Patrycja
Lach, Michał Stefan
Ustaszewski, Adam
Kulcenty, Katarzyna
Ibbs, Matthew
Jagiełło, Inga
Suchorska, Wiktoria Maria
Marszałek, Andrzej
The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
title The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
title_full The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
title_fullStr The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
title_full_unstemmed The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
title_short The Potential Role of Selected miRNA in Uveal Melanoma Primary Tumors as Early Biomarkers of Disease Progression
title_sort potential role of selected mirna in uveal melanoma primary tumors as early biomarkers of disease progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140886/
https://www.ncbi.nlm.nih.gov/pubmed/32131485
http://dx.doi.org/10.3390/genes11030271
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