Cargando…

Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)

The radish is a highly self-incompatible plant, and consequently it is difficult to produce homozygous lines. Bud pollination in cross-fertilization plants should be done by opening immature pollen and attaching pollen to mature flowers. It accordingly takes a lot of time and effort to develop lines...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Kyeongmin, Kang, Yuna, Lee, Sol-Ji, Choi, Se-Hyun, Jeon, Dong-Hyun, Park, Min-Young, Park, Suhyoung, Lim, Yong Pyo, Kim, Changsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141118/
https://www.ncbi.nlm.nih.gov/pubmed/32245207
http://dx.doi.org/10.3390/genes11030337
_version_ 1783519127813488640
author Kim, Kyeongmin
Kang, Yuna
Lee, Sol-Ji
Choi, Se-Hyun
Jeon, Dong-Hyun
Park, Min-Young
Park, Suhyoung
Lim, Yong Pyo
Kim, Changsoo
author_facet Kim, Kyeongmin
Kang, Yuna
Lee, Sol-Ji
Choi, Se-Hyun
Jeon, Dong-Hyun
Park, Min-Young
Park, Suhyoung
Lim, Yong Pyo
Kim, Changsoo
author_sort Kim, Kyeongmin
collection PubMed
description The radish is a highly self-incompatible plant, and consequently it is difficult to produce homozygous lines. Bud pollination in cross-fertilization plants should be done by opening immature pollen and attaching pollen to mature flowers. It accordingly takes a lot of time and effort to develop lines with fixed alleles. In the current study, a haploid breeding method has been applied to obtain homozygous plants in a short period of time by doubling chromosomes through the induction of a plant body in the haploid cells, in order to shorten the time to breed inbred lines. We constructed genetic maps with an F1 population derived by crossing parents that show a superior and inferior ability to regenerate microspores, respectively. Genetic maps were constructed from the maternal and parental maps, separately, using the two-way pseudo-testcross model. The phenotype of the regeneration rate was examined by microspore cultures and a quantitative trait loci (QTL) analysis was performed based on the regeneration rate. From the results of the culture of microspores in the F1 population, more than half of the group did not regenerate, and only a few showed a high regeneration rate. A total of five significant QTLs were detected in the F1 population, and five candidate genes were found based on the results. These candidate genes are divided into two classes, and appear to be related to either PRC2 subunits or auxin synthesis.
format Online
Article
Text
id pubmed-7141118
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71411182020-04-10 Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish) Kim, Kyeongmin Kang, Yuna Lee, Sol-Ji Choi, Se-Hyun Jeon, Dong-Hyun Park, Min-Young Park, Suhyoung Lim, Yong Pyo Kim, Changsoo Genes (Basel) Article The radish is a highly self-incompatible plant, and consequently it is difficult to produce homozygous lines. Bud pollination in cross-fertilization plants should be done by opening immature pollen and attaching pollen to mature flowers. It accordingly takes a lot of time and effort to develop lines with fixed alleles. In the current study, a haploid breeding method has been applied to obtain homozygous plants in a short period of time by doubling chromosomes through the induction of a plant body in the haploid cells, in order to shorten the time to breed inbred lines. We constructed genetic maps with an F1 population derived by crossing parents that show a superior and inferior ability to regenerate microspores, respectively. Genetic maps were constructed from the maternal and parental maps, separately, using the two-way pseudo-testcross model. The phenotype of the regeneration rate was examined by microspore cultures and a quantitative trait loci (QTL) analysis was performed based on the regeneration rate. From the results of the culture of microspores in the F1 population, more than half of the group did not regenerate, and only a few showed a high regeneration rate. A total of five significant QTLs were detected in the F1 population, and five candidate genes were found based on the results. These candidate genes are divided into two classes, and appear to be related to either PRC2 subunits or auxin synthesis. MDPI 2020-03-21 /pmc/articles/PMC7141118/ /pubmed/32245207 http://dx.doi.org/10.3390/genes11030337 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Kyeongmin
Kang, Yuna
Lee, Sol-Ji
Choi, Se-Hyun
Jeon, Dong-Hyun
Park, Min-Young
Park, Suhyoung
Lim, Yong Pyo
Kim, Changsoo
Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)
title Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)
title_full Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)
title_fullStr Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)
title_full_unstemmed Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)
title_short Quantitative Trait Loci (QTLs) Associated with Microspore Culture in Raphanus sativus L. (Radish)
title_sort quantitative trait loci (qtls) associated with microspore culture in raphanus sativus l. (radish)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141118/
https://www.ncbi.nlm.nih.gov/pubmed/32245207
http://dx.doi.org/10.3390/genes11030337
work_keys_str_mv AT kimkyeongmin quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT kangyuna quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT leesolji quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT choisehyun quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT jeondonghyun quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT parkminyoung quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT parksuhyoung quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT limyongpyo quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish
AT kimchangsoo quantitativetraitlociqtlsassociatedwithmicrosporecultureinraphanussativuslradish