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A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies

To determine the plasma metabolomic profile of exudative age-related macular degeneration (AMD), we performed a targeted metabolomics study on the plasma from patients (n = 40, mean age = 81.1) compared to an age- and sex-matched control group (n = 40, mean age = 81.8). All included patients had doc...

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Autores principales: Chao de la Barca, Juan M., Rondet-Courbis, Barnabé, Ferré, Marc, Muller, Jeanne, Buisset, Adrien, Leruez, Stéphanie, Plubeau, Guillaume, Macé, Thibaut, Moureauzeau, Laurie, Chupin, Stéphanie, Tessier, Lydie, Blanchet, Odile, Lenaers, Guy, Procaccio, Vincent, Mirebeau-Prunier, Delphine, Simard, Gilles, Gohier, Philippe, Miléa, Dan, Reynier, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141125/
https://www.ncbi.nlm.nih.gov/pubmed/32120889
http://dx.doi.org/10.3390/jcm9030631
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author Chao de la Barca, Juan M.
Rondet-Courbis, Barnabé
Ferré, Marc
Muller, Jeanne
Buisset, Adrien
Leruez, Stéphanie
Plubeau, Guillaume
Macé, Thibaut
Moureauzeau, Laurie
Chupin, Stéphanie
Tessier, Lydie
Blanchet, Odile
Lenaers, Guy
Procaccio, Vincent
Mirebeau-Prunier, Delphine
Simard, Gilles
Gohier, Philippe
Miléa, Dan
Reynier, Pascal
author_facet Chao de la Barca, Juan M.
Rondet-Courbis, Barnabé
Ferré, Marc
Muller, Jeanne
Buisset, Adrien
Leruez, Stéphanie
Plubeau, Guillaume
Macé, Thibaut
Moureauzeau, Laurie
Chupin, Stéphanie
Tessier, Lydie
Blanchet, Odile
Lenaers, Guy
Procaccio, Vincent
Mirebeau-Prunier, Delphine
Simard, Gilles
Gohier, Philippe
Miléa, Dan
Reynier, Pascal
author_sort Chao de la Barca, Juan M.
collection PubMed
description To determine the plasma metabolomic profile of exudative age-related macular degeneration (AMD), we performed a targeted metabolomics study on the plasma from patients (n = 40, mean age = 81.1) compared to an age- and sex-matched control group (n = 40, mean age = 81.8). All included patients had documented exudative AMD, causing significant visual loss (mean logMAR visual acuity = 0.63), compared to the control group. Patients and controls did not differ in terms of body mass index and co-morbidities. Among the 188 metabolites analyzed, 150 (79.8%) were accurately measured. The concentrations of 18 metabolites were significantly modified in the AMD group, but only six of them remained significantly different after Benjamini–Hochberg correction. Valine, lysine, carnitine, valerylcarnitine and proline were increased, while carnosine, a dipeptide disclosing anti-oxidant and anti-glycating properties, was, on average, reduced by 50% in AMD compared to controls. Moreover, carnosine was undetectable for 49% of AMD patients compared to 18% in the control group (p-value = 0.0035). Carnitine is involved in the transfer of fatty acids within the mitochondria; proline, lysine and valerylcarnitine are substrates for mitochondrial electrons transferring flavoproteins, and proline is one of the main metabolites supplying energy to the retina. Overall, our results reveal six new metabolites involved in the plasma metabolomic profile of exudative AMD, suggesting mitochondrial energetic impairments and carnosine deficiency.
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spelling pubmed-71411252020-04-10 A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies Chao de la Barca, Juan M. Rondet-Courbis, Barnabé Ferré, Marc Muller, Jeanne Buisset, Adrien Leruez, Stéphanie Plubeau, Guillaume Macé, Thibaut Moureauzeau, Laurie Chupin, Stéphanie Tessier, Lydie Blanchet, Odile Lenaers, Guy Procaccio, Vincent Mirebeau-Prunier, Delphine Simard, Gilles Gohier, Philippe Miléa, Dan Reynier, Pascal J Clin Med Article To determine the plasma metabolomic profile of exudative age-related macular degeneration (AMD), we performed a targeted metabolomics study on the plasma from patients (n = 40, mean age = 81.1) compared to an age- and sex-matched control group (n = 40, mean age = 81.8). All included patients had documented exudative AMD, causing significant visual loss (mean logMAR visual acuity = 0.63), compared to the control group. Patients and controls did not differ in terms of body mass index and co-morbidities. Among the 188 metabolites analyzed, 150 (79.8%) were accurately measured. The concentrations of 18 metabolites were significantly modified in the AMD group, but only six of them remained significantly different after Benjamini–Hochberg correction. Valine, lysine, carnitine, valerylcarnitine and proline were increased, while carnosine, a dipeptide disclosing anti-oxidant and anti-glycating properties, was, on average, reduced by 50% in AMD compared to controls. Moreover, carnosine was undetectable for 49% of AMD patients compared to 18% in the control group (p-value = 0.0035). Carnitine is involved in the transfer of fatty acids within the mitochondria; proline, lysine and valerylcarnitine are substrates for mitochondrial electrons transferring flavoproteins, and proline is one of the main metabolites supplying energy to the retina. Overall, our results reveal six new metabolites involved in the plasma metabolomic profile of exudative AMD, suggesting mitochondrial energetic impairments and carnosine deficiency. MDPI 2020-02-27 /pmc/articles/PMC7141125/ /pubmed/32120889 http://dx.doi.org/10.3390/jcm9030631 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chao de la Barca, Juan M.
Rondet-Courbis, Barnabé
Ferré, Marc
Muller, Jeanne
Buisset, Adrien
Leruez, Stéphanie
Plubeau, Guillaume
Macé, Thibaut
Moureauzeau, Laurie
Chupin, Stéphanie
Tessier, Lydie
Blanchet, Odile
Lenaers, Guy
Procaccio, Vincent
Mirebeau-Prunier, Delphine
Simard, Gilles
Gohier, Philippe
Miléa, Dan
Reynier, Pascal
A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies
title A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies
title_full A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies
title_fullStr A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies
title_full_unstemmed A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies
title_short A Plasma Metabolomic Profiling of Exudative Age-Related Macular Degeneration Showing Carnosine and Mitochondrial Deficiencies
title_sort plasma metabolomic profiling of exudative age-related macular degeneration showing carnosine and mitochondrial deficiencies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141125/
https://www.ncbi.nlm.nih.gov/pubmed/32120889
http://dx.doi.org/10.3390/jcm9030631
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