Cargando…

Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens

GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low...

Descripción completa

Detalles Bibliográficos
Autores principales: Ali, Niaz, Chen, Hua, Zhang, Chong, Khan, Shahid Ali, Gandeka, Mamadou, Xie, Dongyang, Zhuang, Weijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141132/
https://www.ncbi.nlm.nih.gov/pubmed/32213970
http://dx.doi.org/10.3390/genes11030343
_version_ 1783519131131183104
author Ali, Niaz
Chen, Hua
Zhang, Chong
Khan, Shahid Ali
Gandeka, Mamadou
Xie, Dongyang
Zhuang, Weijian
author_facet Ali, Niaz
Chen, Hua
Zhang, Chong
Khan, Shahid Ali
Gandeka, Mamadou
Xie, Dongyang
Zhuang, Weijian
author_sort Ali, Niaz
collection PubMed
description GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses.
format Online
Article
Text
id pubmed-7141132
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71411322020-04-10 Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens Ali, Niaz Chen, Hua Zhang, Chong Khan, Shahid Ali Gandeka, Mamadou Xie, Dongyang Zhuang, Weijian Genes (Basel) Article GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses. MDPI 2020-03-24 /pmc/articles/PMC7141132/ /pubmed/32213970 http://dx.doi.org/10.3390/genes11030343 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ali, Niaz
Chen, Hua
Zhang, Chong
Khan, Shahid Ali
Gandeka, Mamadou
Xie, Dongyang
Zhuang, Weijian
Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
title Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
title_full Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
title_fullStr Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
title_full_unstemmed Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
title_short Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
title_sort ectopic expression of ahglk1b (golden2-like transcription factor) in arabidopsis confers dual resistance to fungal and bacterial pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141132/
https://www.ncbi.nlm.nih.gov/pubmed/32213970
http://dx.doi.org/10.3390/genes11030343
work_keys_str_mv AT aliniaz ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens
AT chenhua ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens
AT zhangchong ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens
AT khanshahidali ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens
AT gandekamamadou ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens
AT xiedongyang ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens
AT zhuangweijian ectopicexpressionofahglk1bgolden2liketranscriptionfactorinarabidopsisconfersdualresistancetofungalandbacterialpathogens