Cargando…
Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells
The availability of autologous adult stem cells is one of the essential prerequisites for human stem cell therapy. Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific USCs are easily and non-invasively obtained from urine. Efficient isolat...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141314/ https://www.ncbi.nlm.nih.gov/pubmed/32197458 http://dx.doi.org/10.3390/jcm9030827 |
_version_ | 1783519170976022528 |
---|---|
author | Kim, Kyeongseok Gil, Minchan Dayem, Ahmed Abdal Choi, Sangbaek Kang, Geun-Ho Yang, Gwang-Mo Cho, Sungha Jeong, Yeojin Kim, Se Jong Seok, Jaekwon Kwak, Hee Jeong Kumar Saha, Subbroto Kim, Aram Cho, Ssang-Goo |
author_facet | Kim, Kyeongseok Gil, Minchan Dayem, Ahmed Abdal Choi, Sangbaek Kang, Geun-Ho Yang, Gwang-Mo Cho, Sungha Jeong, Yeojin Kim, Se Jong Seok, Jaekwon Kwak, Hee Jeong Kumar Saha, Subbroto Kim, Aram Cho, Ssang-Goo |
author_sort | Kim, Kyeongseok |
collection | PubMed |
description | The availability of autologous adult stem cells is one of the essential prerequisites for human stem cell therapy. Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific USCs are easily and non-invasively obtained from urine. Efficient isolation, expansion, and differentiation methods of USCs are necessary to increase their availability. Here, we developed a method for efficient isolation and expansion of USCs using Matrigel, and the rho-associated protein kinase (ROCK) inhibitor, Y-27632. The prepared USCs showed significantly enhanced migration, colony forming capacity, and differentiation into osteogenic or chondrogenic lineage. The USCs were successfully reprogramed into induced pluripotent stem cells (USC-iPSCs) and further differentiated into kidney organoid and hematopoietic progenitor cells (HPCs). Using flavonoid molecules, the isolation efficiency of USCs and the production of HPCs from the USC-iPSCs was increased. Taken together, we present an improved isolation method of USCs utilizing Matrigel, a ROCK inhibitor and flavonoids, and enhanced differentiation of USC-iPSC to HPC by flavonoids. These novel findings could significantly enhance the use of USCs and USC-iPSCs for stem cell research and further application in regenerative stem cell-based therapies. |
format | Online Article Text |
id | pubmed-7141314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71413142020-04-10 Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells Kim, Kyeongseok Gil, Minchan Dayem, Ahmed Abdal Choi, Sangbaek Kang, Geun-Ho Yang, Gwang-Mo Cho, Sungha Jeong, Yeojin Kim, Se Jong Seok, Jaekwon Kwak, Hee Jeong Kumar Saha, Subbroto Kim, Aram Cho, Ssang-Goo J Clin Med Article The availability of autologous adult stem cells is one of the essential prerequisites for human stem cell therapy. Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific USCs are easily and non-invasively obtained from urine. Efficient isolation, expansion, and differentiation methods of USCs are necessary to increase their availability. Here, we developed a method for efficient isolation and expansion of USCs using Matrigel, and the rho-associated protein kinase (ROCK) inhibitor, Y-27632. The prepared USCs showed significantly enhanced migration, colony forming capacity, and differentiation into osteogenic or chondrogenic lineage. The USCs were successfully reprogramed into induced pluripotent stem cells (USC-iPSCs) and further differentiated into kidney organoid and hematopoietic progenitor cells (HPCs). Using flavonoid molecules, the isolation efficiency of USCs and the production of HPCs from the USC-iPSCs was increased. Taken together, we present an improved isolation method of USCs utilizing Matrigel, a ROCK inhibitor and flavonoids, and enhanced differentiation of USC-iPSC to HPC by flavonoids. These novel findings could significantly enhance the use of USCs and USC-iPSCs for stem cell research and further application in regenerative stem cell-based therapies. MDPI 2020-03-18 /pmc/articles/PMC7141314/ /pubmed/32197458 http://dx.doi.org/10.3390/jcm9030827 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Kyeongseok Gil, Minchan Dayem, Ahmed Abdal Choi, Sangbaek Kang, Geun-Ho Yang, Gwang-Mo Cho, Sungha Jeong, Yeojin Kim, Se Jong Seok, Jaekwon Kwak, Hee Jeong Kumar Saha, Subbroto Kim, Aram Cho, Ssang-Goo Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells |
title | Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells |
title_full | Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells |
title_fullStr | Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells |
title_full_unstemmed | Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells |
title_short | Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells |
title_sort | improved isolation and culture of urine-derived stem cells (uscs) and enhanced production of immune cells from the usc-derived induced pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141314/ https://www.ncbi.nlm.nih.gov/pubmed/32197458 http://dx.doi.org/10.3390/jcm9030827 |
work_keys_str_mv | AT kimkyeongseok improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT gilminchan improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT dayemahmedabdal improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT choisangbaek improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT kanggeunho improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT yanggwangmo improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT chosungha improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT jeongyeojin improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT kimsejong improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT seokjaekwon improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT kwakheejeong improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT kumarsahasubbroto improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT kimaram improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells AT chossanggoo improvedisolationandcultureofurinederivedstemcellsuscsandenhancedproductionofimmunecellsfromtheuscderivedinducedpluripotentstemcells |