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Nanoerythrosome-functionalized biohybrid microswimmers

Biohybrid microswimmers, which are realized through the integration of motile microscopic organisms with artificial cargo carriers, have a significant potential to revolutionize autonomous targeted cargo delivery applications in medicine. Nonetheless, there are many open challenges, such as motility...

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Detalles Bibliográficos
Autores principales: Buss, Nicole, Yasa, Oncay, Alapan, Yunus, Akolpoglu, Mukrime Birgul, Sitti, Metin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141839/
https://www.ncbi.nlm.nih.gov/pubmed/32548539
http://dx.doi.org/10.1063/1.5130670
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author Buss, Nicole
Yasa, Oncay
Alapan, Yunus
Akolpoglu, Mukrime Birgul
Sitti, Metin
author_facet Buss, Nicole
Yasa, Oncay
Alapan, Yunus
Akolpoglu, Mukrime Birgul
Sitti, Metin
author_sort Buss, Nicole
collection PubMed
description Biohybrid microswimmers, which are realized through the integration of motile microscopic organisms with artificial cargo carriers, have a significant potential to revolutionize autonomous targeted cargo delivery applications in medicine. Nonetheless, there are many open challenges, such as motility performance and immunogenicity of the biological segment of the microswimmers, which should be overcome before their successful transition to the clinic. Here, we present the design and characterization of a biohybrid microswimmer, which is composed of a genetically engineered peritrichously flagellated Escherichia coli species integrated with red blood cell-derived nanoliposomes, also known as nanoerythrosomes. Initially, we demonstrated nanoerythrosome fabrication using the cell extrusion technique and characterization of their size and functional cell membrane proteins with dynamic light scattering and flow cytometry analyses, respectively. Then, we showed the construction of biohybrid microswimmers through the conjugation of streptavidin-modified bacteria with biotin-modified nanoerythrosomes by using non-covalent streptavidin interaction. Finally, we investigated the motility performance of the nanoerythrosome-functionalized biohybrid microswimmers and compared it with the free-swimming bacteria. The microswimmer design approach presented here could lead to the fabrication of personalized biohybrid microswimmers from patients' own cells with high fabrication efficiencies and motility performances.
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spelling pubmed-71418392020-06-15 Nanoerythrosome-functionalized biohybrid microswimmers Buss, Nicole Yasa, Oncay Alapan, Yunus Akolpoglu, Mukrime Birgul Sitti, Metin APL Bioeng Articles Biohybrid microswimmers, which are realized through the integration of motile microscopic organisms with artificial cargo carriers, have a significant potential to revolutionize autonomous targeted cargo delivery applications in medicine. Nonetheless, there are many open challenges, such as motility performance and immunogenicity of the biological segment of the microswimmers, which should be overcome before their successful transition to the clinic. Here, we present the design and characterization of a biohybrid microswimmer, which is composed of a genetically engineered peritrichously flagellated Escherichia coli species integrated with red blood cell-derived nanoliposomes, also known as nanoerythrosomes. Initially, we demonstrated nanoerythrosome fabrication using the cell extrusion technique and characterization of their size and functional cell membrane proteins with dynamic light scattering and flow cytometry analyses, respectively. Then, we showed the construction of biohybrid microswimmers through the conjugation of streptavidin-modified bacteria with biotin-modified nanoerythrosomes by using non-covalent streptavidin interaction. Finally, we investigated the motility performance of the nanoerythrosome-functionalized biohybrid microswimmers and compared it with the free-swimming bacteria. The microswimmer design approach presented here could lead to the fabrication of personalized biohybrid microswimmers from patients' own cells with high fabrication efficiencies and motility performances. AIP Publishing LLC 2020-04-07 /pmc/articles/PMC7141839/ /pubmed/32548539 http://dx.doi.org/10.1063/1.5130670 Text en © Author(s). 2473-2877/2020/4(2)/026103/9 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Buss, Nicole
Yasa, Oncay
Alapan, Yunus
Akolpoglu, Mukrime Birgul
Sitti, Metin
Nanoerythrosome-functionalized biohybrid microswimmers
title Nanoerythrosome-functionalized biohybrid microswimmers
title_full Nanoerythrosome-functionalized biohybrid microswimmers
title_fullStr Nanoerythrosome-functionalized biohybrid microswimmers
title_full_unstemmed Nanoerythrosome-functionalized biohybrid microswimmers
title_short Nanoerythrosome-functionalized biohybrid microswimmers
title_sort nanoerythrosome-functionalized biohybrid microswimmers
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141839/
https://www.ncbi.nlm.nih.gov/pubmed/32548539
http://dx.doi.org/10.1063/1.5130670
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