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Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans
The homeobox domain-containing transcription factors play an important role in the growth, development, and secondary metabolism in fungi and other eukaryotes. In this study, we characterized the roles of the genes coding for homeobox-type proteins in the model organism Aspergillus nidulans. To exam...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142095/ https://www.ncbi.nlm.nih.gov/pubmed/32269291 http://dx.doi.org/10.1038/s41598-020-63300-4 |
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author | Son, Sung-Hun Son, Ye-Eun Cho, He-Jin Chen, Wanping Lee, Mi-Kyung Kim, Lee-Han Han, Dong-Min Park, Hee-Soo |
author_facet | Son, Sung-Hun Son, Ye-Eun Cho, He-Jin Chen, Wanping Lee, Mi-Kyung Kim, Lee-Han Han, Dong-Min Park, Hee-Soo |
author_sort | Son, Sung-Hun |
collection | PubMed |
description | The homeobox domain-containing transcription factors play an important role in the growth, development, and secondary metabolism in fungi and other eukaryotes. In this study, we characterized the roles of the genes coding for homeobox-type proteins in the model organism Aspergillus nidulans. To examine their roles in A. nidulans, the deletion mutant strains for each gene coding for homeobox-type protein were generated, and their phenotypes were examined. Phenotypic analyses revealed that two homeobox proteins, HbxA and HbxB, were required for conidia production. Deletion of hbxA caused abnormal conidiophore production, decreased the number of conidia in both light and dark conditions, and decreased the size of cleistothecia structures. Overexpressing hbxA enhanced the production of asexual spores and formation of conidiophore under the liquid submerged conditions. The hbxB deletion mutant strains exhibited decreased asexual spore production but increased cleistothecia production. The absence of hbxB decreased the trehalose content in asexual spores and increased their sensitivity against thermal and oxidative stresses. The ΔhbxA strains produced more sterigmatocystin, which was decreased in the ΔhbxB strain. Overall, our results show that HbxA and HbxB play crucial roles in the differentiation and secondary metabolism of the fungus A. nidulans. |
format | Online Article Text |
id | pubmed-7142095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71420952020-04-11 Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans Son, Sung-Hun Son, Ye-Eun Cho, He-Jin Chen, Wanping Lee, Mi-Kyung Kim, Lee-Han Han, Dong-Min Park, Hee-Soo Sci Rep Article The homeobox domain-containing transcription factors play an important role in the growth, development, and secondary metabolism in fungi and other eukaryotes. In this study, we characterized the roles of the genes coding for homeobox-type proteins in the model organism Aspergillus nidulans. To examine their roles in A. nidulans, the deletion mutant strains for each gene coding for homeobox-type protein were generated, and their phenotypes were examined. Phenotypic analyses revealed that two homeobox proteins, HbxA and HbxB, were required for conidia production. Deletion of hbxA caused abnormal conidiophore production, decreased the number of conidia in both light and dark conditions, and decreased the size of cleistothecia structures. Overexpressing hbxA enhanced the production of asexual spores and formation of conidiophore under the liquid submerged conditions. The hbxB deletion mutant strains exhibited decreased asexual spore production but increased cleistothecia production. The absence of hbxB decreased the trehalose content in asexual spores and increased their sensitivity against thermal and oxidative stresses. The ΔhbxA strains produced more sterigmatocystin, which was decreased in the ΔhbxB strain. Overall, our results show that HbxA and HbxB play crucial roles in the differentiation and secondary metabolism of the fungus A. nidulans. Nature Publishing Group UK 2020-04-08 /pmc/articles/PMC7142095/ /pubmed/32269291 http://dx.doi.org/10.1038/s41598-020-63300-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Son, Sung-Hun Son, Ye-Eun Cho, He-Jin Chen, Wanping Lee, Mi-Kyung Kim, Lee-Han Han, Dong-Min Park, Hee-Soo Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans |
title | Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans |
title_full | Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans |
title_fullStr | Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans |
title_full_unstemmed | Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans |
title_short | Homeobox proteins are essential for fungal differentiation and secondary metabolism in Aspergillus nidulans |
title_sort | homeobox proteins are essential for fungal differentiation and secondary metabolism in aspergillus nidulans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142095/ https://www.ncbi.nlm.nih.gov/pubmed/32269291 http://dx.doi.org/10.1038/s41598-020-63300-4 |
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