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Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis
TAC1 and LAZY1 are members of a gene family that regulates lateral shoot orientation in plants. TAC1 promotes outward orientations in response to light, while LAZY1 promotes upward shoot orientations in response to gravity via altered auxin transport. We performed genetic, molecular, and biochemical...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142156/ https://www.ncbi.nlm.nih.gov/pubmed/32269265 http://dx.doi.org/10.1038/s41598-020-62962-4 |
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author | Hollender, Courtney A. Hill, Joseph L. Waite, Jessica Dardick, Chris |
author_facet | Hollender, Courtney A. Hill, Joseph L. Waite, Jessica Dardick, Chris |
author_sort | Hollender, Courtney A. |
collection | PubMed |
description | TAC1 and LAZY1 are members of a gene family that regulates lateral shoot orientation in plants. TAC1 promotes outward orientations in response to light, while LAZY1 promotes upward shoot orientations in response to gravity via altered auxin transport. We performed genetic, molecular, and biochemical assays to investigate possible interactions between these genes. In Arabidopsis they were expressed in similar tissues and double mutants revealed the wide-angled lazy1 branch phenotype, indicating it is epistatic to the tac1 shoot phenotype. Surprisingly, the lack of TAC1 did not influence gravitropic shoot curvature responses. Combined, these results suggest TAC1 might negatively regulate LAZY1 to promote outward shoot orientations. However, additional results revealed that TAC1- and LAZY1 influence on shoot orientation is more complex than a simple direct negative regulatory pathway. Transcriptomes of Arabidopsis tac1 and lazy1 mutants compared to wild type under normal and gravistimulated conditions revealed few overlapping differentially expressed genes. Overexpression of each gene did not result in major branch angle differences. Shoot tip hormone levels were similar between tac1, lazy1, and Col, apart from exceptionally elevated levels of salicylic acid in lazy1. The data presented here provide a foundation for future study of TAC1 and LAZY1 regulation of shoot architecture. |
format | Online Article Text |
id | pubmed-7142156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71421562020-04-15 Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis Hollender, Courtney A. Hill, Joseph L. Waite, Jessica Dardick, Chris Sci Rep Article TAC1 and LAZY1 are members of a gene family that regulates lateral shoot orientation in plants. TAC1 promotes outward orientations in response to light, while LAZY1 promotes upward shoot orientations in response to gravity via altered auxin transport. We performed genetic, molecular, and biochemical assays to investigate possible interactions between these genes. In Arabidopsis they were expressed in similar tissues and double mutants revealed the wide-angled lazy1 branch phenotype, indicating it is epistatic to the tac1 shoot phenotype. Surprisingly, the lack of TAC1 did not influence gravitropic shoot curvature responses. Combined, these results suggest TAC1 might negatively regulate LAZY1 to promote outward shoot orientations. However, additional results revealed that TAC1- and LAZY1 influence on shoot orientation is more complex than a simple direct negative regulatory pathway. Transcriptomes of Arabidopsis tac1 and lazy1 mutants compared to wild type under normal and gravistimulated conditions revealed few overlapping differentially expressed genes. Overexpression of each gene did not result in major branch angle differences. Shoot tip hormone levels were similar between tac1, lazy1, and Col, apart from exceptionally elevated levels of salicylic acid in lazy1. The data presented here provide a foundation for future study of TAC1 and LAZY1 regulation of shoot architecture. Nature Publishing Group UK 2020-04-08 /pmc/articles/PMC7142156/ /pubmed/32269265 http://dx.doi.org/10.1038/s41598-020-62962-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hollender, Courtney A. Hill, Joseph L. Waite, Jessica Dardick, Chris Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis |
title | Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis |
title_full | Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis |
title_fullStr | Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis |
title_full_unstemmed | Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis |
title_short | Opposing influences of TAC1 and LAZY1 on Lateral Shoot Orientation in Arabidopsis |
title_sort | opposing influences of tac1 and lazy1 on lateral shoot orientation in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142156/ https://www.ncbi.nlm.nih.gov/pubmed/32269265 http://dx.doi.org/10.1038/s41598-020-62962-4 |
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