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Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection

Powdery mildew infection of wheat (Triticum aestivum L.), caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease that threatens yield and quality worldwide. The most effective and preferred means for the control of the disease is to identify broad-spectrum resistance genes for br...

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Autores principales: Jia, Mengshu, Xu, Hongxing, Liu, Cheng, Mao, Ruixi, Li, Haosheng, Liu, Jianjun, Du, Wenxiao, Wang, Wenrui, Zhang, Xu, Han, Ran, Wang, Xiaolu, Wu, Liru, Liang, Xiao, Song, Jiancheng, He, Huagang, Ma, Pengtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142250/
https://www.ncbi.nlm.nih.gov/pubmed/32300355
http://dx.doi.org/10.3389/fgene.2020.00241
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author Jia, Mengshu
Xu, Hongxing
Liu, Cheng
Mao, Ruixi
Li, Haosheng
Liu, Jianjun
Du, Wenxiao
Wang, Wenrui
Zhang, Xu
Han, Ran
Wang, Xiaolu
Wu, Liru
Liang, Xiao
Song, Jiancheng
He, Huagang
Ma, Pengtao
author_facet Jia, Mengshu
Xu, Hongxing
Liu, Cheng
Mao, Ruixi
Li, Haosheng
Liu, Jianjun
Du, Wenxiao
Wang, Wenrui
Zhang, Xu
Han, Ran
Wang, Xiaolu
Wu, Liru
Liang, Xiao
Song, Jiancheng
He, Huagang
Ma, Pengtao
author_sort Jia, Mengshu
collection PubMed
description Powdery mildew infection of wheat (Triticum aestivum L.), caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease that threatens yield and quality worldwide. The most effective and preferred means for the control of the disease is to identify broad-spectrum resistance genes for breeding, especially the genes derived from elite cultivars that exhibit desirable agronomic traits. Jimai 23 is a Chinese wheat cultivar with superior agronomic performance, high-quality characteristics, and effective resistance to powdery mildew at all growth stages. Genetic analysis indicated that powdery mildew resistance in Jimai 23 was mediated by a single dominant gene, tentatively designated PmJM23. Using bulked segregant RNA-Seq (BSR-Seq), a series of markers was developed and used to map PmJM23. PmJM23 was then located at the Pm2 locus on the short arm of chromosome 5D (5DS). Resistance spectrum analysis demonstrated that PmJM23 provided a broad resistance spectrum different from that of the documented Pm2 alleles, indicating that PmJM23 is most likely a new allele of Pm2. In view of these combined agronomic, quality, and resistance findings, PmJM23 is expected to be a valuable resistance gene in wheat breeding. To efficiently use PmJM23 in breeding, the closely linked markers of PmJM23 were evaluated and confirmed to be applicable for marker-assisted selection (MAS). Using these markers, a series of resistant breeding lines with high resistance and desirable agronomic performance was selected from the crosses involving PmJM23, resulting in improved powdery mildew resistance of these lines.
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spelling pubmed-71422502020-04-16 Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection Jia, Mengshu Xu, Hongxing Liu, Cheng Mao, Ruixi Li, Haosheng Liu, Jianjun Du, Wenxiao Wang, Wenrui Zhang, Xu Han, Ran Wang, Xiaolu Wu, Liru Liang, Xiao Song, Jiancheng He, Huagang Ma, Pengtao Front Genet Genetics Powdery mildew infection of wheat (Triticum aestivum L.), caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease that threatens yield and quality worldwide. The most effective and preferred means for the control of the disease is to identify broad-spectrum resistance genes for breeding, especially the genes derived from elite cultivars that exhibit desirable agronomic traits. Jimai 23 is a Chinese wheat cultivar with superior agronomic performance, high-quality characteristics, and effective resistance to powdery mildew at all growth stages. Genetic analysis indicated that powdery mildew resistance in Jimai 23 was mediated by a single dominant gene, tentatively designated PmJM23. Using bulked segregant RNA-Seq (BSR-Seq), a series of markers was developed and used to map PmJM23. PmJM23 was then located at the Pm2 locus on the short arm of chromosome 5D (5DS). Resistance spectrum analysis demonstrated that PmJM23 provided a broad resistance spectrum different from that of the documented Pm2 alleles, indicating that PmJM23 is most likely a new allele of Pm2. In view of these combined agronomic, quality, and resistance findings, PmJM23 is expected to be a valuable resistance gene in wheat breeding. To efficiently use PmJM23 in breeding, the closely linked markers of PmJM23 were evaluated and confirmed to be applicable for marker-assisted selection (MAS). Using these markers, a series of resistant breeding lines with high resistance and desirable agronomic performance was selected from the crosses involving PmJM23, resulting in improved powdery mildew resistance of these lines. Frontiers Media S.A. 2020-04-02 /pmc/articles/PMC7142250/ /pubmed/32300355 http://dx.doi.org/10.3389/fgene.2020.00241 Text en Copyright © 2020 Jia, Xu, Liu, Mao, Li, Liu, Du, Wang, Zhang, Han, Wang, Wu, Liang, Song, He and Ma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Jia, Mengshu
Xu, Hongxing
Liu, Cheng
Mao, Ruixi
Li, Haosheng
Liu, Jianjun
Du, Wenxiao
Wang, Wenrui
Zhang, Xu
Han, Ran
Wang, Xiaolu
Wu, Liru
Liang, Xiao
Song, Jiancheng
He, Huagang
Ma, Pengtao
Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection
title Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection
title_full Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection
title_fullStr Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection
title_full_unstemmed Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection
title_short Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection
title_sort characterization of the powdery mildew resistance gene in the elite wheat cultivar jimai 23 and its application in marker-assisted selection
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142250/
https://www.ncbi.nlm.nih.gov/pubmed/32300355
http://dx.doi.org/10.3389/fgene.2020.00241
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