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Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury

Immunization with neural derived peptides (INDP), as well as scar removal (SR) and the use of matrices with bone marrow-mesenchymal stem cells (MSCs), have been studied separately and proven to induce a functional and morphological improvement after spinal cord injury (SCI). Herein, we evaluated the...

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Autores principales: Rodríguez-Barrera, Roxana, Flores-Romero, Adrián, Buzoianu-Anguiano, Vinnitsa, Garcia, Elisa, Soria-Zavala, Karla, Incontri-Abraham, Diego, Garibay-López, Marcela, Juárez-Vignon Whaley, Juan José, Ibarra, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142263/
https://www.ncbi.nlm.nih.gov/pubmed/32300328
http://dx.doi.org/10.3389/fneur.2020.00189
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author Rodríguez-Barrera, Roxana
Flores-Romero, Adrián
Buzoianu-Anguiano, Vinnitsa
Garcia, Elisa
Soria-Zavala, Karla
Incontri-Abraham, Diego
Garibay-López, Marcela
Juárez-Vignon Whaley, Juan José
Ibarra, Antonio
author_facet Rodríguez-Barrera, Roxana
Flores-Romero, Adrián
Buzoianu-Anguiano, Vinnitsa
Garcia, Elisa
Soria-Zavala, Karla
Incontri-Abraham, Diego
Garibay-López, Marcela
Juárez-Vignon Whaley, Juan José
Ibarra, Antonio
author_sort Rodríguez-Barrera, Roxana
collection PubMed
description Immunization with neural derived peptides (INDP), as well as scar removal (SR) and the use of matrices with bone marrow-mesenchymal stem cells (MSCs), have been studied separately and proven to induce a functional and morphological improvement after spinal cord injury (SCI). Herein, we evaluated the therapeutic effects of INDP combined with SR and a fibrin glue matrix (FGM) with MSCs (FGM-MSCs), on motor recovery, axonal regeneration-associated molecules and cytokine expression, axonal regeneration (catecholaminergic and serotonergic fibers), and the induction of neurogenesis after a chronic SCI. For this purpose, female adult Sprague-Dawley rats were subjected to SCI, 60 days after lesion, rats were randomly distributed in four groups: (1) Rats immunized with complete Freund's adjuvant + PBS (vehicle; PBS-I); (2) Rats with SR+ FGM-MSCs; (3) Rats with SR+ INDP + FGM-MSCs; (4) Rats only with INDP. Afterwards, we evaluated motor recovery using the BBB locomotor test. Sixty days after the therapy, protein expression of TNFα, IL-4, IL-10, BDNF, and GAP-43 were evaluated using ELISA assay. The number of catecholaminergic and serotonergic fibers were also determined. Neurogenesis was evaluated through immunofluorescence. The results show that treatment with INDP alone significantly increased motor recovery, anti-inflammatory cytokines, regeneration-associated molecules, axonal regeneration, and neurogenesis when compared to the rest of the groups. Our findings suggest that the combination therapy (SR + INDP + FGM-MSCs) modifies the non-permissive microenvironment post SCI, but it is not capable of inducing an appropriate axonal regeneration or neurogenesis when compared to the treatment with INDP alone.
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spelling pubmed-71422632020-04-16 Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury Rodríguez-Barrera, Roxana Flores-Romero, Adrián Buzoianu-Anguiano, Vinnitsa Garcia, Elisa Soria-Zavala, Karla Incontri-Abraham, Diego Garibay-López, Marcela Juárez-Vignon Whaley, Juan José Ibarra, Antonio Front Neurol Neurology Immunization with neural derived peptides (INDP), as well as scar removal (SR) and the use of matrices with bone marrow-mesenchymal stem cells (MSCs), have been studied separately and proven to induce a functional and morphological improvement after spinal cord injury (SCI). Herein, we evaluated the therapeutic effects of INDP combined with SR and a fibrin glue matrix (FGM) with MSCs (FGM-MSCs), on motor recovery, axonal regeneration-associated molecules and cytokine expression, axonal regeneration (catecholaminergic and serotonergic fibers), and the induction of neurogenesis after a chronic SCI. For this purpose, female adult Sprague-Dawley rats were subjected to SCI, 60 days after lesion, rats were randomly distributed in four groups: (1) Rats immunized with complete Freund's adjuvant + PBS (vehicle; PBS-I); (2) Rats with SR+ FGM-MSCs; (3) Rats with SR+ INDP + FGM-MSCs; (4) Rats only with INDP. Afterwards, we evaluated motor recovery using the BBB locomotor test. Sixty days after the therapy, protein expression of TNFα, IL-4, IL-10, BDNF, and GAP-43 were evaluated using ELISA assay. The number of catecholaminergic and serotonergic fibers were also determined. Neurogenesis was evaluated through immunofluorescence. The results show that treatment with INDP alone significantly increased motor recovery, anti-inflammatory cytokines, regeneration-associated molecules, axonal regeneration, and neurogenesis when compared to the rest of the groups. Our findings suggest that the combination therapy (SR + INDP + FGM-MSCs) modifies the non-permissive microenvironment post SCI, but it is not capable of inducing an appropriate axonal regeneration or neurogenesis when compared to the treatment with INDP alone. Frontiers Media S.A. 2020-04-02 /pmc/articles/PMC7142263/ /pubmed/32300328 http://dx.doi.org/10.3389/fneur.2020.00189 Text en Copyright © 2020 Rodríguez-Barrera, Flores-Romero, Buzoianu-Anguiano, Garcia, Soria-Zavala, Incontri-Abraham, Garibay-López, Juárez-Vignon Whaley and Ibarra. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neurology
Rodríguez-Barrera, Roxana
Flores-Romero, Adrián
Buzoianu-Anguiano, Vinnitsa
Garcia, Elisa
Soria-Zavala, Karla
Incontri-Abraham, Diego
Garibay-López, Marcela
Juárez-Vignon Whaley, Juan José
Ibarra, Antonio
Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury
title Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury
title_full Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury
title_fullStr Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury
title_full_unstemmed Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury
title_short Use of a Combination Strategy to Improve Morphological and Functional Recovery in Rats With Chronic Spinal Cord Injury
title_sort use of a combination strategy to improve morphological and functional recovery in rats with chronic spinal cord injury
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142263/
https://www.ncbi.nlm.nih.gov/pubmed/32300328
http://dx.doi.org/10.3389/fneur.2020.00189
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