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B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus

Cells expressing the spike (S) glycoprotein of the neurotropic JHM strain (JHMV) of mouse hepatitis virus (MHV) are susceptible to lysis by B cells derived from naïve mice, including B cells from perforin-deficient mice. Cytolysis requires interaction of the virus receptor and the viral S glycoprote...

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Autores principales: Morales, Shawn, Parra, Beatriz, Ramakrishna, Chandran, Blau, Dianna M., Stohlman, Stephen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press. 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142306/
https://www.ncbi.nlm.nih.gov/pubmed/11448169
http://dx.doi.org/10.1006/viro.2001.0991
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author Morales, Shawn
Parra, Beatriz
Ramakrishna, Chandran
Blau, Dianna M.
Stohlman, Stephen A.
author_facet Morales, Shawn
Parra, Beatriz
Ramakrishna, Chandran
Blau, Dianna M.
Stohlman, Stephen A.
author_sort Morales, Shawn
collection PubMed
description Cells expressing the spike (S) glycoprotein of the neurotropic JHM strain (JHMV) of mouse hepatitis virus (MHV) are susceptible to lysis by B cells derived from naïve mice, including B cells from perforin-deficient mice. Cytolysis requires interaction of the virus receptor and the viral S glycoprotein, is independent of other viral-induced components, and is not a unique property of B cells. Neutralizing anti-S-protein monoclonal antibodies (mAb) and a mAb specific for the viral receptor inhibit lysis. However, cells infected with an MHV strain unable to induce cell–cell fusion are resistant to lysis and lysis of JHMV-infected cells is inhibited by an anti-S-protein nonneutralizing mAb which prevents S-protein-mediated cell fusion. These data suggest that B cells may function as antibody-independent innate immune response during JHMV infection in vivo.
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spelling pubmed-71423062020-04-09 B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus Morales, Shawn Parra, Beatriz Ramakrishna, Chandran Blau, Dianna M. Stohlman, Stephen A. Virology Regular Article Cells expressing the spike (S) glycoprotein of the neurotropic JHM strain (JHMV) of mouse hepatitis virus (MHV) are susceptible to lysis by B cells derived from naïve mice, including B cells from perforin-deficient mice. Cytolysis requires interaction of the virus receptor and the viral S glycoprotein, is independent of other viral-induced components, and is not a unique property of B cells. Neutralizing anti-S-protein monoclonal antibodies (mAb) and a mAb specific for the viral receptor inhibit lysis. However, cells infected with an MHV strain unable to induce cell–cell fusion are resistant to lysis and lysis of JHMV-infected cells is inhibited by an anti-S-protein nonneutralizing mAb which prevents S-protein-mediated cell fusion. These data suggest that B cells may function as antibody-independent innate immune response during JHMV infection in vivo. Academic Press. 2001-07-20 2002-05-25 /pmc/articles/PMC7142306/ /pubmed/11448169 http://dx.doi.org/10.1006/viro.2001.0991 Text en Copyright © 2001 Academic Press. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Regular Article
Morales, Shawn
Parra, Beatriz
Ramakrishna, Chandran
Blau, Dianna M.
Stohlman, Stephen A.
B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus
title B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus
title_full B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus
title_fullStr B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus
title_full_unstemmed B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus
title_short B-Cell-Mediated Lysis of Cells Infected with the Neurotropic JHM Strain of Mouse Hepatitis Virus
title_sort b-cell-mediated lysis of cells infected with the neurotropic jhm strain of mouse hepatitis virus
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142306/
https://www.ncbi.nlm.nih.gov/pubmed/11448169
http://dx.doi.org/10.1006/viro.2001.0991
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