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Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity
PURPOSE: Simple methodology for preparation of metal nanoparticles such as AgNPs uses an methanolic aqueous medium at room temperature or a solvent-free procedure under microwave irradiation. The prepared AgNPs showed a significant antimicrobial effect against Gram-positive bacteria, Gram-negative b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142329/ https://www.ncbi.nlm.nih.gov/pubmed/32308387 http://dx.doi.org/10.2147/IJN.S244678 |
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author | El-Faham, Ayman Al-Rasheed, Hessa H Sholkamy, Essam N Osman, Sameh M ALOthman, Zeid A |
author_facet | El-Faham, Ayman Al-Rasheed, Hessa H Sholkamy, Essam N Osman, Sameh M ALOthman, Zeid A |
author_sort | El-Faham, Ayman |
collection | PubMed |
description | PURPOSE: Simple methodology for preparation of metal nanoparticles such as AgNPs uses an methanolic aqueous medium at room temperature or a solvent-free procedure under microwave irradiation. The prepared AgNPs showed a significant antimicrobial effect against Gram-positive bacteria, Gram-negative bacteria, and fungi. METHODS: The modified methoxypolyethylene glycol bishydrazino-s-triazine (mPEGTH2) showed remarkable activity for reducing Ag(+) to Ag(0) in an aqueous methanolic solution and using a solvent-free method (solid phase) under microwave irradiation. In the solid phase synthesis, the size and shape of the AgNPs can be controlled by varying the weight ratio of mPEGTH2 to AgNO(3) used. In addition, the antimicrobial activity depends on the ratio of mPEGTH2 to AgNO(3). The mPEGTH2-AgNPs (2:1) demonstrated higher antimicrobial activity compared to mPEGTH2-AgNPs (1:1) against Gram-positive bacteria, Gram-negative bacteria, and C.albicans. RESULTS: This work presents simple methods for the synthesis of AgNPs using modified methoxypolyethylene glycol with bishydrazino-s-triazine (mPEGTH2); a solution method, using methanol-water medium at room temperature, and a solvent-free (solid phase) method, employing microwave irradiation or direct heating which could be used for the preparation of AgNPs on large scale. In the solid phase, ratios of mPEGTH2 to AgNO(3) (1:1 or 2:1, respectively) are very important to control the size and shape of AgNPs. While in solution phase is not necessary where the molar ratio used is 10:1. Most of the experimental methods resulted in AgNPs ranging in size from 7 to 10 nm as observed from XRD and TEM characterization. The antimicrobial activity of the AgNPs was also dependent on the weight ratio of mPEGTH2 to AgNO(3), with a large effect as observed when using the solvent-free method. The mPEGTH2-AgNPs (2:1) demonstrated higher antimicrobial activities compared to mPEGTH2-AgNPs (1:1) against S. aureus, S. epidermidis, E. faecalis, E. coli, P. aeruginosa, S. typhimurium, and C. albicans. In all cases, the MICs and MBCs of mPEGTH2-AgNPs (1:1) were lower than those of mPEGTH2-AgNPs (2:1). CONCLUSION: In summary, mPEGTH2-AgNPs (2:1) is a promising candidate to kill pathogenic microbes. In particular, the method used for the preparation of AgNPs by using polyethylene glycol polymer modified with bishydrazino-s-triazine has the most potential and would be the most cost-effective method. This method of the synthesis of nanoparticles may be suitable for the preparation of other metal nanoparticles, which would allow for numerous applications in medicinal and industrial. |
format | Online Article Text |
id | pubmed-7142329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-71423292020-04-17 Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity El-Faham, Ayman Al-Rasheed, Hessa H Sholkamy, Essam N Osman, Sameh M ALOthman, Zeid A Int J Nanomedicine Original Research PURPOSE: Simple methodology for preparation of metal nanoparticles such as AgNPs uses an methanolic aqueous medium at room temperature or a solvent-free procedure under microwave irradiation. The prepared AgNPs showed a significant antimicrobial effect against Gram-positive bacteria, Gram-negative bacteria, and fungi. METHODS: The modified methoxypolyethylene glycol bishydrazino-s-triazine (mPEGTH2) showed remarkable activity for reducing Ag(+) to Ag(0) in an aqueous methanolic solution and using a solvent-free method (solid phase) under microwave irradiation. In the solid phase synthesis, the size and shape of the AgNPs can be controlled by varying the weight ratio of mPEGTH2 to AgNO(3) used. In addition, the antimicrobial activity depends on the ratio of mPEGTH2 to AgNO(3). The mPEGTH2-AgNPs (2:1) demonstrated higher antimicrobial activity compared to mPEGTH2-AgNPs (1:1) against Gram-positive bacteria, Gram-negative bacteria, and C.albicans. RESULTS: This work presents simple methods for the synthesis of AgNPs using modified methoxypolyethylene glycol with bishydrazino-s-triazine (mPEGTH2); a solution method, using methanol-water medium at room temperature, and a solvent-free (solid phase) method, employing microwave irradiation or direct heating which could be used for the preparation of AgNPs on large scale. In the solid phase, ratios of mPEGTH2 to AgNO(3) (1:1 or 2:1, respectively) are very important to control the size and shape of AgNPs. While in solution phase is not necessary where the molar ratio used is 10:1. Most of the experimental methods resulted in AgNPs ranging in size from 7 to 10 nm as observed from XRD and TEM characterization. The antimicrobial activity of the AgNPs was also dependent on the weight ratio of mPEGTH2 to AgNO(3), with a large effect as observed when using the solvent-free method. The mPEGTH2-AgNPs (2:1) demonstrated higher antimicrobial activities compared to mPEGTH2-AgNPs (1:1) against S. aureus, S. epidermidis, E. faecalis, E. coli, P. aeruginosa, S. typhimurium, and C. albicans. In all cases, the MICs and MBCs of mPEGTH2-AgNPs (1:1) were lower than those of mPEGTH2-AgNPs (2:1). CONCLUSION: In summary, mPEGTH2-AgNPs (2:1) is a promising candidate to kill pathogenic microbes. In particular, the method used for the preparation of AgNPs by using polyethylene glycol polymer modified with bishydrazino-s-triazine has the most potential and would be the most cost-effective method. This method of the synthesis of nanoparticles may be suitable for the preparation of other metal nanoparticles, which would allow for numerous applications in medicinal and industrial. Dove 2020-04-03 /pmc/articles/PMC7142329/ /pubmed/32308387 http://dx.doi.org/10.2147/IJN.S244678 Text en © 2020 El-Faham et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research El-Faham, Ayman Al-Rasheed, Hessa H Sholkamy, Essam N Osman, Sameh M ALOthman, Zeid A Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity |
title | Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity |
title_full | Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity |
title_fullStr | Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity |
title_full_unstemmed | Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity |
title_short | Simple Approaches for the Synthesis of AgNPs in Solution and Solid Phase Using Modified Methoxypolyethylene Glycol and Evaluation of Their Antimicrobial Activity |
title_sort | simple approaches for the synthesis of agnps in solution and solid phase using modified methoxypolyethylene glycol and evaluation of their antimicrobial activity |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142329/ https://www.ncbi.nlm.nih.gov/pubmed/32308387 http://dx.doi.org/10.2147/IJN.S244678 |
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