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Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device

Microfluidics has gained a lot of attention for biological sample separation and purification methods over recent years. From many active and passive microfluidic techniques, inertial microfluidics offers a simple and efficient method to demonstrate various biological applications. One prevalent lim...

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Detalles Bibliográficos
Autores principales: Bogseth, Amanda, Zhou, Jian, Papautsky, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142704/
https://www.ncbi.nlm.nih.gov/pubmed/32164264
http://dx.doi.org/10.3390/mi11030287
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author Bogseth, Amanda
Zhou, Jian
Papautsky, Ian
author_facet Bogseth, Amanda
Zhou, Jian
Papautsky, Ian
author_sort Bogseth, Amanda
collection PubMed
description Microfluidics has gained a lot of attention for biological sample separation and purification methods over recent years. From many active and passive microfluidic techniques, inertial microfluidics offers a simple and efficient method to demonstrate various biological applications. One prevalent limitation of this method is its lack of tunability for different applications once the microfluidic devices are fabricated. In this work, we develop and characterize a co-flow inertial microfluidic device that is tunable in multiple ways for adaptation to different application requirements. In particular, flow rate, flow rate ratio and output resistance ratio are systematically evaluated for flexibility of the cutoff size of the device and modification of the separation performance post-fabrication. Typically, a mixture of single size particles is used to determine cutoff sizes for the outlets, yet this fails to provide accurate prediction for efficiency and purity for a more complex biological sample. Thus, we use particles with continuous size distribution (2–32 μm) for separation demonstration under conditions of various flow rates, flow rate ratios and resistance ratios. We also use A549 cancer cell line with continuous size distribution (12–27 μm) as an added demonstration. Our results indicate inertial microfluidic devices possess the tunability that offers multiple ways to improve device performance for adaptation to different applications even after the devices are prototyped.
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spelling pubmed-71427042020-04-15 Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device Bogseth, Amanda Zhou, Jian Papautsky, Ian Micromachines (Basel) Article Microfluidics has gained a lot of attention for biological sample separation and purification methods over recent years. From many active and passive microfluidic techniques, inertial microfluidics offers a simple and efficient method to demonstrate various biological applications. One prevalent limitation of this method is its lack of tunability for different applications once the microfluidic devices are fabricated. In this work, we develop and characterize a co-flow inertial microfluidic device that is tunable in multiple ways for adaptation to different application requirements. In particular, flow rate, flow rate ratio and output resistance ratio are systematically evaluated for flexibility of the cutoff size of the device and modification of the separation performance post-fabrication. Typically, a mixture of single size particles is used to determine cutoff sizes for the outlets, yet this fails to provide accurate prediction for efficiency and purity for a more complex biological sample. Thus, we use particles with continuous size distribution (2–32 μm) for separation demonstration under conditions of various flow rates, flow rate ratios and resistance ratios. We also use A549 cancer cell line with continuous size distribution (12–27 μm) as an added demonstration. Our results indicate inertial microfluidic devices possess the tunability that offers multiple ways to improve device performance for adaptation to different applications even after the devices are prototyped. MDPI 2020-03-10 /pmc/articles/PMC7142704/ /pubmed/32164264 http://dx.doi.org/10.3390/mi11030287 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bogseth, Amanda
Zhou, Jian
Papautsky, Ian
Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device
title Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device
title_full Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device
title_fullStr Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device
title_full_unstemmed Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device
title_short Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device
title_sort evaluation of performance and tunability of a co-flow inertial microfluidic device
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142704/
https://www.ncbi.nlm.nih.gov/pubmed/32164264
http://dx.doi.org/10.3390/mi11030287
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