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Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy

Helicobacter pylori (Hp) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious Hp strains. Focusing on a high GC ri...

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Autores principales: Toracchio, Sonia, Caruso, Rosario Alberto, Perconti, Silvia, Rigoli, Luciana, Betri, Enrico, Neri, Matteo, Verginelli, Fabio, Mariani-Costantini, Renato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142731/
https://www.ncbi.nlm.nih.gov/pubmed/32110918
http://dx.doi.org/10.3390/microorganisms8030324
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author Toracchio, Sonia
Caruso, Rosario Alberto
Perconti, Silvia
Rigoli, Luciana
Betri, Enrico
Neri, Matteo
Verginelli, Fabio
Mariani-Costantini, Renato
author_facet Toracchio, Sonia
Caruso, Rosario Alberto
Perconti, Silvia
Rigoli, Luciana
Betri, Enrico
Neri, Matteo
Verginelli, Fabio
Mariani-Costantini, Renato
author_sort Toracchio, Sonia
collection PubMed
description Helicobacter pylori (Hp) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious Hp strains. Focusing on a high GC risk area of Northern Italy (Cremona, Lombardy) and using archived paraffin-embedded biopsies, we investigated the associations between the Hp vacA and cagA genotype variants and gastric intraepithelial neoplasia (GIN, 33 cases) versus non-neoplastic gastroduodenal lesions (NNGDLs, 37 cases). The glmM gene and the cagA and vacA (s and m) genotypes were determined by polymerase chain reaction (PCR) and sequencing. Hp was confirmed in 37/37 (100%) NNGDLs and detected in 9/33 GINs (27%), consistently with the well-known Hp loss in GC. CagA was detected in 4/9 Hp-positive GINs and in 29/37 NNGDLs. The vacA s1a and m1 subtypes were more common in GINs than in NNGDLs (6/7 vs. 12/34, p=0.014, for s1a; 7/7 vs. 18/34, p=0.020 for m1), with significant vacA s genotype-specific variance. The GIN-associated vacA s1a sequences clustered together, suggesting that aggressive Hp strains from a unique founder contribute to GC in the high-risk area studied.
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spelling pubmed-71427312020-04-15 Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy Toracchio, Sonia Caruso, Rosario Alberto Perconti, Silvia Rigoli, Luciana Betri, Enrico Neri, Matteo Verginelli, Fabio Mariani-Costantini, Renato Microorganisms Article Helicobacter pylori (Hp) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious Hp strains. Focusing on a high GC risk area of Northern Italy (Cremona, Lombardy) and using archived paraffin-embedded biopsies, we investigated the associations between the Hp vacA and cagA genotype variants and gastric intraepithelial neoplasia (GIN, 33 cases) versus non-neoplastic gastroduodenal lesions (NNGDLs, 37 cases). The glmM gene and the cagA and vacA (s and m) genotypes were determined by polymerase chain reaction (PCR) and sequencing. Hp was confirmed in 37/37 (100%) NNGDLs and detected in 9/33 GINs (27%), consistently with the well-known Hp loss in GC. CagA was detected in 4/9 Hp-positive GINs and in 29/37 NNGDLs. The vacA s1a and m1 subtypes were more common in GINs than in NNGDLs (6/7 vs. 12/34, p=0.014, for s1a; 7/7 vs. 18/34, p=0.020 for m1), with significant vacA s genotype-specific variance. The GIN-associated vacA s1a sequences clustered together, suggesting that aggressive Hp strains from a unique founder contribute to GC in the high-risk area studied. MDPI 2020-02-26 /pmc/articles/PMC7142731/ /pubmed/32110918 http://dx.doi.org/10.3390/microorganisms8030324 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Toracchio, Sonia
Caruso, Rosario Alberto
Perconti, Silvia
Rigoli, Luciana
Betri, Enrico
Neri, Matteo
Verginelli, Fabio
Mariani-Costantini, Renato
Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
title Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
title_full Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
title_fullStr Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
title_full_unstemmed Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
title_short Evolutionarily-Related Helicobacter pylori Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
title_sort evolutionarily-related helicobacter pylori genotypes and gastric intraepithelial neoplasia in a high-risk area of northern italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142731/
https://www.ncbi.nlm.nih.gov/pubmed/32110918
http://dx.doi.org/10.3390/microorganisms8030324
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