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Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance

Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of the bacterial pathogen Acinetobacter baumannii on dry surfaces. Here we explore several factors influencing desiccation survival of A. baumannii. At the macroscale level, we find that desiccation...

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Detalles Bibliográficos
Autores principales: Wang, Xun, Cole, Cody G., DuPai, Cory D., Davies, Bryan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142981/
https://www.ncbi.nlm.nih.gov/pubmed/32121206
http://dx.doi.org/10.3390/microorganisms8030343
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author Wang, Xun
Cole, Cody G.
DuPai, Cory D.
Davies, Bryan W.
author_facet Wang, Xun
Cole, Cody G.
DuPai, Cory D.
Davies, Bryan W.
author_sort Wang, Xun
collection PubMed
description Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of the bacterial pathogen Acinetobacter baumannii on dry surfaces. Here we explore several factors influencing desiccation survival of A. baumannii. At the macroscale level, we find that desiccation tolerance is influenced by cell density and growth phase. A transcriptome analysis indicates that desiccation represents a unique state for A. baumannii compared to commonly studied growth phases and strongly influences pathways responsible for proteostasis. Remarkably, we find that an increase in total cellular protein aggregates, which is often considered deleterious, correlates positively with the ability of A. baumannii to survive desiccation. We show that inducing protein aggregate formation prior to desiccation increases survival and, importantly, that proteins incorporated into cellular aggregates can retain activity. Our results suggest that protein aggregates may promote desiccation tolerance in A. baumannii through preserving and protecting proteins from damage during desiccation until rehydration occurs.
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spelling pubmed-71429812020-04-14 Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance Wang, Xun Cole, Cody G. DuPai, Cory D. Davies, Bryan W. Microorganisms Article Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of the bacterial pathogen Acinetobacter baumannii on dry surfaces. Here we explore several factors influencing desiccation survival of A. baumannii. At the macroscale level, we find that desiccation tolerance is influenced by cell density and growth phase. A transcriptome analysis indicates that desiccation represents a unique state for A. baumannii compared to commonly studied growth phases and strongly influences pathways responsible for proteostasis. Remarkably, we find that an increase in total cellular protein aggregates, which is often considered deleterious, correlates positively with the ability of A. baumannii to survive desiccation. We show that inducing protein aggregate formation prior to desiccation increases survival and, importantly, that proteins incorporated into cellular aggregates can retain activity. Our results suggest that protein aggregates may promote desiccation tolerance in A. baumannii through preserving and protecting proteins from damage during desiccation until rehydration occurs. MDPI 2020-02-28 /pmc/articles/PMC7142981/ /pubmed/32121206 http://dx.doi.org/10.3390/microorganisms8030343 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xun
Cole, Cody G.
DuPai, Cory D.
Davies, Bryan W.
Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance
title Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance
title_full Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance
title_fullStr Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance
title_full_unstemmed Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance
title_short Protein Aggregation is Associated with Acinetobacter baumannii Desiccation Tolerance
title_sort protein aggregation is associated with acinetobacter baumannii desiccation tolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142981/
https://www.ncbi.nlm.nih.gov/pubmed/32121206
http://dx.doi.org/10.3390/microorganisms8030343
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