Cargando…

A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus

Previous studies have revealed that some proteins in Bombyx mori larvae digestive juice show antiviral activity. Here, based on the label-free proteomics data, BmLipase member H-A (BmLHA) was identified as being involved in the response to BmNPV infection in B. mori larvae digestive juice. In the pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Shang-Zhi, Zhu, Lin-Bao, You, Ling-Ling, Wang, Jie, Cao, Hui-Hua, Liu, Ying-Xue, Toufeeq, Shahzad, Wang, Yu-Ling, Kong, Xue, Xu, Jia-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143000/
https://www.ncbi.nlm.nih.gov/pubmed/32121517
http://dx.doi.org/10.3390/insects11030154
_version_ 1783519512026415104
author Zhang, Shang-Zhi
Zhu, Lin-Bao
You, Ling-Ling
Wang, Jie
Cao, Hui-Hua
Liu, Ying-Xue
Toufeeq, Shahzad
Wang, Yu-Ling
Kong, Xue
Xu, Jia-Ping
author_facet Zhang, Shang-Zhi
Zhu, Lin-Bao
You, Ling-Ling
Wang, Jie
Cao, Hui-Hua
Liu, Ying-Xue
Toufeeq, Shahzad
Wang, Yu-Ling
Kong, Xue
Xu, Jia-Ping
author_sort Zhang, Shang-Zhi
collection PubMed
description Previous studies have revealed that some proteins in Bombyx mori larvae digestive juice show antiviral activity. Here, based on the label-free proteomics data, BmLipase member H-A (BmLHA) was identified as being involved in the response to BmNPV infection in B. mori larvae digestive juice. In the present study, a gene encoding the BmLHA protein in B. mori was characterized. The protein has an open reading fragment of 999 bp, encoding a predicted 332 amino acid residue-protein with a molecular weight of approximately 35.9 kDa. The phylogenetic analysis revealed that BmLHA shares a close genetic distance with Papilio xuthus Lipase member H-A. BmLHA was highly expressed in the middle part of the B. mori gut, and the expression level increased with instar rising in larvae. There was higher expression of BmLHA in A35 than in P50 strains, and it was upregulated in both A35 and P50 strains, following BmNPV infection. The expression level of VP39 decreased significantly in appropriate recombinant-BmLHA-treated groups compared with the PBS-treated group in B. mori larvae and BmN cells. Meanwhile, overexpression of BmLHA significantly reduced the infectivity of BmNPV in BmN cells. These results indicated that BmLHA did not have digestive function but had anti-BmNPV activity. Taken together, our work provides valuable data for the clarification of the molecular characterization BmLHA and supplements research on proteins of anti-BmNPV activity in B. mori.
format Online
Article
Text
id pubmed-7143000
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71430002020-04-14 A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus Zhang, Shang-Zhi Zhu, Lin-Bao You, Ling-Ling Wang, Jie Cao, Hui-Hua Liu, Ying-Xue Toufeeq, Shahzad Wang, Yu-Ling Kong, Xue Xu, Jia-Ping Insects Article Previous studies have revealed that some proteins in Bombyx mori larvae digestive juice show antiviral activity. Here, based on the label-free proteomics data, BmLipase member H-A (BmLHA) was identified as being involved in the response to BmNPV infection in B. mori larvae digestive juice. In the present study, a gene encoding the BmLHA protein in B. mori was characterized. The protein has an open reading fragment of 999 bp, encoding a predicted 332 amino acid residue-protein with a molecular weight of approximately 35.9 kDa. The phylogenetic analysis revealed that BmLHA shares a close genetic distance with Papilio xuthus Lipase member H-A. BmLHA was highly expressed in the middle part of the B. mori gut, and the expression level increased with instar rising in larvae. There was higher expression of BmLHA in A35 than in P50 strains, and it was upregulated in both A35 and P50 strains, following BmNPV infection. The expression level of VP39 decreased significantly in appropriate recombinant-BmLHA-treated groups compared with the PBS-treated group in B. mori larvae and BmN cells. Meanwhile, overexpression of BmLHA significantly reduced the infectivity of BmNPV in BmN cells. These results indicated that BmLHA did not have digestive function but had anti-BmNPV activity. Taken together, our work provides valuable data for the clarification of the molecular characterization BmLHA and supplements research on proteins of anti-BmNPV activity in B. mori. MDPI 2020-03-01 /pmc/articles/PMC7143000/ /pubmed/32121517 http://dx.doi.org/10.3390/insects11030154 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Shang-Zhi
Zhu, Lin-Bao
You, Ling-Ling
Wang, Jie
Cao, Hui-Hua
Liu, Ying-Xue
Toufeeq, Shahzad
Wang, Yu-Ling
Kong, Xue
Xu, Jia-Ping
A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
title A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
title_full A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
title_fullStr A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
title_full_unstemmed A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
title_short A Novel Digestive Proteinase Lipase Member H-A in Bombyx mori Contributes to Digestive Juice Antiviral Activity against B. mori Nucleopolyhedrovirus
title_sort novel digestive proteinase lipase member h-a in bombyx mori contributes to digestive juice antiviral activity against b. mori nucleopolyhedrovirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143000/
https://www.ncbi.nlm.nih.gov/pubmed/32121517
http://dx.doi.org/10.3390/insects11030154
work_keys_str_mv AT zhangshangzhi anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT zhulinbao anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT youlingling anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT wangjie anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT caohuihua anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT liuyingxue anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT toufeeqshahzad anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT wangyuling anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT kongxue anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT xujiaping anoveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT zhangshangzhi noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT zhulinbao noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT youlingling noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT wangjie noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT caohuihua noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT liuyingxue noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT toufeeqshahzad noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT wangyuling noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT kongxue noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus
AT xujiaping noveldigestiveproteinaselipasememberhainbombyxmoricontributestodigestivejuiceantiviralactivityagainstbmorinucleopolyhedrovirus