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Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol

The association between household air pollution and lower respiratory tract infections (LRTI) among children under five years of age has been well documented; however, the extent to which the microbiome within the indoor environment contributes to this association is uncertain. The home assessment o...

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Autores principales: Fakunle, Adekunle G., Olusola, Babatunde, Jafta, Nkosana, Faneye, Adedayo, Heederik, Dick, Smit, Lidwien A.M., Naidoo, Rajen N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143126/
https://www.ncbi.nlm.nih.gov/pubmed/32183028
http://dx.doi.org/10.3390/ijerph17061857
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author Fakunle, Adekunle G.
Olusola, Babatunde
Jafta, Nkosana
Faneye, Adedayo
Heederik, Dick
Smit, Lidwien A.M.
Naidoo, Rajen N.
author_facet Fakunle, Adekunle G.
Olusola, Babatunde
Jafta, Nkosana
Faneye, Adedayo
Heederik, Dick
Smit, Lidwien A.M.
Naidoo, Rajen N.
author_sort Fakunle, Adekunle G.
collection PubMed
description The association between household air pollution and lower respiratory tract infections (LRTI) among children under five years of age has been well documented; however, the extent to which the microbiome within the indoor environment contributes to this association is uncertain. The home assessment of indoor microbiome (HAIM) study seeks to assess the abundance of indoor microbiota (IM) in the homes of under-five children (U-5Cs) with and without LRTI. HAIM is a hospital- and community-based study involving 200 cases and 200 controls recruited from three children’s hospitals in Ibadan, Nigeria. Cases will be hospital-based patients with LRTI confirmed by a pediatrician, while controls will be community-based participants, matched to cases on the basis of sex, geographical location, and age (±3 months) without LRTI. The abundance of IM in houses of cases and controls will be investigated using active and passive air sampling techniques and analyzed by qualitative detection of bacterial 16SrRNA gene (V3–V4), fungal ITS1 region, and viral RNA sequencing. HAIM is expected to elucidate the relationship between exposure to IM and incidence of LRTI among U-5Cs and ultimately provide evidence base for strategic interventions to curtail the burgeoning burden of LRTI on the subcontinent.
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spelling pubmed-71431262020-04-14 Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol Fakunle, Adekunle G. Olusola, Babatunde Jafta, Nkosana Faneye, Adedayo Heederik, Dick Smit, Lidwien A.M. Naidoo, Rajen N. Int J Environ Res Public Health Protocol The association between household air pollution and lower respiratory tract infections (LRTI) among children under five years of age has been well documented; however, the extent to which the microbiome within the indoor environment contributes to this association is uncertain. The home assessment of indoor microbiome (HAIM) study seeks to assess the abundance of indoor microbiota (IM) in the homes of under-five children (U-5Cs) with and without LRTI. HAIM is a hospital- and community-based study involving 200 cases and 200 controls recruited from three children’s hospitals in Ibadan, Nigeria. Cases will be hospital-based patients with LRTI confirmed by a pediatrician, while controls will be community-based participants, matched to cases on the basis of sex, geographical location, and age (±3 months) without LRTI. The abundance of IM in houses of cases and controls will be investigated using active and passive air sampling techniques and analyzed by qualitative detection of bacterial 16SrRNA gene (V3–V4), fungal ITS1 region, and viral RNA sequencing. HAIM is expected to elucidate the relationship between exposure to IM and incidence of LRTI among U-5Cs and ultimately provide evidence base for strategic interventions to curtail the burgeoning burden of LRTI on the subcontinent. MDPI 2020-03-13 2020-03 /pmc/articles/PMC7143126/ /pubmed/32183028 http://dx.doi.org/10.3390/ijerph17061857 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Fakunle, Adekunle G.
Olusola, Babatunde
Jafta, Nkosana
Faneye, Adedayo
Heederik, Dick
Smit, Lidwien A.M.
Naidoo, Rajen N.
Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
title Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
title_full Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
title_fullStr Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
title_full_unstemmed Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
title_short Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
title_sort home assessment of indoor microbiome (haim) in relation to lower respiratory tract infections among under-five children in ibadan, nigeria: the study protocol
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143126/
https://www.ncbi.nlm.nih.gov/pubmed/32183028
http://dx.doi.org/10.3390/ijerph17061857
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