Effects of Different Stroking Styles on Behaviour and Cardiac Parameters in Heifers

SIMPLE SUMMARY: Positive emotions can improve the welfare of animals. Humans can induce positive emotions in cattle via gentle interactions, such as stroking. While previous studies showed that stroking at the lower side of the neck elicited the most positive reactions in cows, cattle groom each other on different body regions and probably react to each other’s signals. We compared the reactions of dairy heifers to two different stroking styles: stroking exclusively on the lower neck or stroking the whole head/neck region and reactively following the signals of the animal. For both styles, we observed longer durations of behaviours indicating positive emotions and relaxation during stroking, suggesting that the animals enjoyed the treatment. The different stroking styles led to differences in the positions of the heifers’ ears: during “reactive” stroking, the animals held their ears longer in low positions, whereas during stroking of the lower neck, the ears spent longer pointing backwards-upwards. However, we did not observe significant differences in other behaviours, indicating that the manner of stroking of the head/neck region seemed to be not very important for the positive perception of stroking. We conclude that both ways of stroking can elicit positive emotions in cattle and increase the animals’ well-being. ABSTRACT: Gentle animal–human interactions, such as stroking, can promote positive emotions and thus welfare in cattle. While previous studies showed that stroking at the ventral neck elicited the most positive reactions in cows, intra-specific allogrooming in cattle includes different body regions and is probably guided partly by the receiver. Thus, we compared heifers’ (n = 28) reactions to stroking with the experimenter either reactively responding to perceived momentary preferences of the heifers or exclusively stroking the ventral neck. Independently of the stroking style, longer durations of neck stretching and contact occurred during stroking, supporting our hypothesis of a positive perception of stroking. We did not confirm the predicted decrease in heart rate and increase in heart rate variability, but instead found a slightly increased mean heart rate during stroking. The different stroking styles elicited differences in the heifers’ ear positions: “reactive” stroking led to longer durations of low ear positions during stroking, while during “ventral neck” stroking, the duration of back up increased. However, no other behaviours differed significantly between different stroking styles, indicating that the exact manner of stroking applied in our treatments seemed to be less important in the promotion of positive affective states in cattle through gentle human–animal interactions.