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Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models

The present study assesses the in vitro antibiofilm potential activity of extracts of wild Allium ursinum and Allium oschaninii. The active ingredients of the extracts were obtained with a technique named Naviglio (rapid solid–liquid dynamic extraction, RSLDE) which is based on an innovative and gre...

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Autores principales: Galdiero, Emilia, Di Onofrio, Valeria, Maione, Angela, Gambino, Edvige, Gesuele, Renato, Menale, Bruno, Ciaravolo, Martina, Carraturo, Federica, Guida, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143215/
https://www.ncbi.nlm.nih.gov/pubmed/32120894
http://dx.doi.org/10.3390/microorganisms8030336
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author Galdiero, Emilia
Di Onofrio, Valeria
Maione, Angela
Gambino, Edvige
Gesuele, Renato
Menale, Bruno
Ciaravolo, Martina
Carraturo, Federica
Guida, Marco
author_facet Galdiero, Emilia
Di Onofrio, Valeria
Maione, Angela
Gambino, Edvige
Gesuele, Renato
Menale, Bruno
Ciaravolo, Martina
Carraturo, Federica
Guida, Marco
author_sort Galdiero, Emilia
collection PubMed
description The present study assesses the in vitro antibiofilm potential activity of extracts of wild Allium ursinum and Allium oschaninii. The active ingredients of the extracts were obtained with a technique named Naviglio (rapid solid–liquid dynamic extraction, RSLDE) which is based on an innovative and green solid–liquid extraction methodology. The extracts were tested against models of mono- and polymicrobial biofilm structures of clinically antibiotic-resistant pathogens, Klebsiella pneumoniae ATCC 10031 and Candida albicans ATCC 90028. Biofilms were studied using a static and a dynamic model (microtiter plates and a CDC reactor) on three different surfaces reproducing what happens on implantable medical devices. Antimicrobic activities were determined through minimum inhibitory concentration (MIC), while antibiofilm activity was assessed by minimum biofilm eradication concentration (MBEC) using a crystal violet (CV) biofilm assay and colony forming unit (CFU) counts. Results showed that both Allium extracts eradicated biofilms of the tested microorganisms well; biofilms on Teflon were more susceptible to extracts than those on polypropylene and polycarbonate, suggesting that when grown on a complex substrate, biofilms may be more tolerant to antibiotics. Our data provide significant advances on antibiotic susceptibility testing of biofilms grown on biologically relevant materials for future in vitro and in vivo applications.
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spelling pubmed-71432152020-04-14 Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models Galdiero, Emilia Di Onofrio, Valeria Maione, Angela Gambino, Edvige Gesuele, Renato Menale, Bruno Ciaravolo, Martina Carraturo, Federica Guida, Marco Microorganisms Article The present study assesses the in vitro antibiofilm potential activity of extracts of wild Allium ursinum and Allium oschaninii. The active ingredients of the extracts were obtained with a technique named Naviglio (rapid solid–liquid dynamic extraction, RSLDE) which is based on an innovative and green solid–liquid extraction methodology. The extracts were tested against models of mono- and polymicrobial biofilm structures of clinically antibiotic-resistant pathogens, Klebsiella pneumoniae ATCC 10031 and Candida albicans ATCC 90028. Biofilms were studied using a static and a dynamic model (microtiter plates and a CDC reactor) on three different surfaces reproducing what happens on implantable medical devices. Antimicrobic activities were determined through minimum inhibitory concentration (MIC), while antibiofilm activity was assessed by minimum biofilm eradication concentration (MBEC) using a crystal violet (CV) biofilm assay and colony forming unit (CFU) counts. Results showed that both Allium extracts eradicated biofilms of the tested microorganisms well; biofilms on Teflon were more susceptible to extracts than those on polypropylene and polycarbonate, suggesting that when grown on a complex substrate, biofilms may be more tolerant to antibiotics. Our data provide significant advances on antibiotic susceptibility testing of biofilms grown on biologically relevant materials for future in vitro and in vivo applications. MDPI 2020-02-27 /pmc/articles/PMC7143215/ /pubmed/32120894 http://dx.doi.org/10.3390/microorganisms8030336 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Galdiero, Emilia
Di Onofrio, Valeria
Maione, Angela
Gambino, Edvige
Gesuele, Renato
Menale, Bruno
Ciaravolo, Martina
Carraturo, Federica
Guida, Marco
Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models
title Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models
title_full Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models
title_fullStr Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models
title_full_unstemmed Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models
title_short Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models
title_sort allium ursinum and allium oschaninii against klebsiella pneumoniae and candida albicans mono- and polymicrobic biofilms in in vitro static and dynamic models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143215/
https://www.ncbi.nlm.nih.gov/pubmed/32120894
http://dx.doi.org/10.3390/microorganisms8030336
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