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Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T)
Despite significant interest and past work to elucidate the phylogeny and photochemistry of species of the Heliobacteriaceae, genomic analyses of heliobacteria to date have been limited to just one published genome, that of the thermophilic species Heliobacterium (Hbt.) modesticaldum str. Ice1(T). H...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143216/ https://www.ncbi.nlm.nih.gov/pubmed/32106460 http://dx.doi.org/10.3390/microorganisms8030313 |
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author | Dewey, Emma D. Stokes, Lynn M. Burchell, Brad M. Shaffer, Kathryn N. Huntington, Austin M. Baker, Jennifer M. Nadendla, Suvarna Giglio, Michelle G. Bender, Kelly S. Touchman, Jeffrey W. Blankenship, Robert E. Madigan, Michael T. Sattley, W. Matthew |
author_facet | Dewey, Emma D. Stokes, Lynn M. Burchell, Brad M. Shaffer, Kathryn N. Huntington, Austin M. Baker, Jennifer M. Nadendla, Suvarna Giglio, Michelle G. Bender, Kelly S. Touchman, Jeffrey W. Blankenship, Robert E. Madigan, Michael T. Sattley, W. Matthew |
author_sort | Dewey, Emma D. |
collection | PubMed |
description | Despite significant interest and past work to elucidate the phylogeny and photochemistry of species of the Heliobacteriaceae, genomic analyses of heliobacteria to date have been limited to just one published genome, that of the thermophilic species Heliobacterium (Hbt.) modesticaldum str. Ice1(T). Here we present an analysis of the complete genome of a second heliobacterium, Heliorestis (Hrs.) convoluta str. HH(T), an alkaliphilic, mesophilic, and morphologically distinct heliobacterium isolated from an Egyptian soda lake. The genome of Hrs. convoluta is a single circular chromosome of 3.22 Mb with a GC content of 43.1% and 3263 protein-encoding genes. In addition to culture-based observations and insights gleaned from the Hbt. modesticaldum genome, an analysis of enzyme-encoding genes from key metabolic pathways supports an obligately photoheterotrophic lifestyle for Hrs. convoluta. A complete set of genes encoding enzymes for propionate and butyrate catabolism and the absence of a gene encoding lactate dehydrogenase distinguishes the carbon metabolism of Hrs. convoluta from its close relatives. Comparative analyses of key proteins in Hrs. convoluta, including cytochrome c(553) and the F(o) alpha subunit of ATP synthase, with those of related species reveal variations in specific amino acid residues that likely contribute to the success of Hrs. convoluta in its highly alkaline environment. |
format | Online Article Text |
id | pubmed-7143216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71432162020-04-14 Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) Dewey, Emma D. Stokes, Lynn M. Burchell, Brad M. Shaffer, Kathryn N. Huntington, Austin M. Baker, Jennifer M. Nadendla, Suvarna Giglio, Michelle G. Bender, Kelly S. Touchman, Jeffrey W. Blankenship, Robert E. Madigan, Michael T. Sattley, W. Matthew Microorganisms Article Despite significant interest and past work to elucidate the phylogeny and photochemistry of species of the Heliobacteriaceae, genomic analyses of heliobacteria to date have been limited to just one published genome, that of the thermophilic species Heliobacterium (Hbt.) modesticaldum str. Ice1(T). Here we present an analysis of the complete genome of a second heliobacterium, Heliorestis (Hrs.) convoluta str. HH(T), an alkaliphilic, mesophilic, and morphologically distinct heliobacterium isolated from an Egyptian soda lake. The genome of Hrs. convoluta is a single circular chromosome of 3.22 Mb with a GC content of 43.1% and 3263 protein-encoding genes. In addition to culture-based observations and insights gleaned from the Hbt. modesticaldum genome, an analysis of enzyme-encoding genes from key metabolic pathways supports an obligately photoheterotrophic lifestyle for Hrs. convoluta. A complete set of genes encoding enzymes for propionate and butyrate catabolism and the absence of a gene encoding lactate dehydrogenase distinguishes the carbon metabolism of Hrs. convoluta from its close relatives. Comparative analyses of key proteins in Hrs. convoluta, including cytochrome c(553) and the F(o) alpha subunit of ATP synthase, with those of related species reveal variations in specific amino acid residues that likely contribute to the success of Hrs. convoluta in its highly alkaline environment. MDPI 2020-02-25 /pmc/articles/PMC7143216/ /pubmed/32106460 http://dx.doi.org/10.3390/microorganisms8030313 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dewey, Emma D. Stokes, Lynn M. Burchell, Brad M. Shaffer, Kathryn N. Huntington, Austin M. Baker, Jennifer M. Nadendla, Suvarna Giglio, Michelle G. Bender, Kelly S. Touchman, Jeffrey W. Blankenship, Robert E. Madigan, Michael T. Sattley, W. Matthew Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) |
title | Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) |
title_full | Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) |
title_fullStr | Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) |
title_full_unstemmed | Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) |
title_short | Analysis of the Complete Genome of the Alkaliphilic and Phototrophic Firmicute Heliorestis convoluta Strain HH(T) |
title_sort | analysis of the complete genome of the alkaliphilic and phototrophic firmicute heliorestis convoluta strain hh(t) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143216/ https://www.ncbi.nlm.nih.gov/pubmed/32106460 http://dx.doi.org/10.3390/microorganisms8030313 |
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