Free Flow Ion Concentration Polarization Focusing (FF-ICPF)

[Image: see text] Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentrat...

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Autores principales: Papadimitriou, Vasileios A., Segerink, Loes I., Eijkel, Jan C. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143306/
https://www.ncbi.nlm.nih.gov/pubmed/32195578
http://dx.doi.org/10.1021/acs.analchem.9b04526
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author Papadimitriou, Vasileios A.
Segerink, Loes I.
Eijkel, Jan C. T.
author_facet Papadimitriou, Vasileios A.
Segerink, Loes I.
Eijkel, Jan C. T.
author_sort Papadimitriou, Vasileios A.
collection PubMed
description [Image: see text] Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min(–1).
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spelling pubmed-71433062020-04-10 Free Flow Ion Concentration Polarization Focusing (FF-ICPF) Papadimitriou, Vasileios A. Segerink, Loes I. Eijkel, Jan C. T. Anal Chem [Image: see text] Electrokinetic separation techniques in microfluidics are a powerful analytical chemistry tool, although an inherent limitation of microfluidics is their low sample throughput. In this article we report a free-flow variant of an electrokinetic focusing method, namely ion concentration polarization focusing (ICPF). The analytes flow continuously through the system via pressure driven flow while they separate and concentrate perpendicularly to the flow by ICPF. We demonstrate free flow ion concentration polarization focusing (FF-ICPF) in two operating modes, namely peak and plateau modes. Additionally, we showed the separation resolution could be improved by the use of an electrophoretic spacer. We report a concentration factor of 10 in human blood plasma in continuous flow at a flow rate of 15 μL min(–1). American Chemical Society 2020-03-20 2020-04-07 /pmc/articles/PMC7143306/ /pubmed/32195578 http://dx.doi.org/10.1021/acs.analchem.9b04526 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Papadimitriou, Vasileios A.
Segerink, Loes I.
Eijkel, Jan C. T.
Free Flow Ion Concentration Polarization Focusing (FF-ICPF)
title Free Flow Ion Concentration Polarization Focusing (FF-ICPF)
title_full Free Flow Ion Concentration Polarization Focusing (FF-ICPF)
title_fullStr Free Flow Ion Concentration Polarization Focusing (FF-ICPF)
title_full_unstemmed Free Flow Ion Concentration Polarization Focusing (FF-ICPF)
title_short Free Flow Ion Concentration Polarization Focusing (FF-ICPF)
title_sort free flow ion concentration polarization focusing (ff-icpf)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143306/
https://www.ncbi.nlm.nih.gov/pubmed/32195578
http://dx.doi.org/10.1021/acs.analchem.9b04526
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