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Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of cells as well as an investigation of on-chip a...

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Autores principales: Thiriet, Pierre-Emmanuel, Pezoldt, Joern, Gambardella, Gabriele, Keim, Kevin, Deplancke, Bart, Guiducci, Carlotta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143322/
https://www.ncbi.nlm.nih.gov/pubmed/32244902
http://dx.doi.org/10.3390/mi11030322
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author Thiriet, Pierre-Emmanuel
Pezoldt, Joern
Gambardella, Gabriele
Keim, Kevin
Deplancke, Bart
Guiducci, Carlotta
author_facet Thiriet, Pierre-Emmanuel
Pezoldt, Joern
Gambardella, Gabriele
Keim, Kevin
Deplancke, Bart
Guiducci, Carlotta
author_sort Thiriet, Pierre-Emmanuel
collection PubMed
description Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of cells as well as an investigation of on-chip analysis on cell phenotype, requirements still largely unmet. Here, we introduce a device for single-cell isolation, selective retrieval and off-chip recovery. To this end, singularly addressable three-dimensional electrodes are embedded within a microfluidic channel, allowing the selective release of single cells from their trapping site through application of a negative dielectrophoretic (DEP) force. Selective capture and release are carried out in standard culture medium and cells can be subsequently mitigated towards a recovery well using micro-engineered hybrid SU-8/PDMS pneumatic valves. Importantly, transcriptional analysis of recovered cells revealed only marginal alteration of their molecular profile upon DEP application, underscored by minor transcriptional changes induced upon injection into the microfluidic device. Therefore, the established microfluidic system combining targeted DEP manipulation with downstream hydrodynamic coordination of single cells provides a powerful means to handle and manipulate individual cells within one device.
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spelling pubmed-71433222020-04-14 Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow Thiriet, Pierre-Emmanuel Pezoldt, Joern Gambardella, Gabriele Keim, Kevin Deplancke, Bart Guiducci, Carlotta Micromachines (Basel) Article Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of cells as well as an investigation of on-chip analysis on cell phenotype, requirements still largely unmet. Here, we introduce a device for single-cell isolation, selective retrieval and off-chip recovery. To this end, singularly addressable three-dimensional electrodes are embedded within a microfluidic channel, allowing the selective release of single cells from their trapping site through application of a negative dielectrophoretic (DEP) force. Selective capture and release are carried out in standard culture medium and cells can be subsequently mitigated towards a recovery well using micro-engineered hybrid SU-8/PDMS pneumatic valves. Importantly, transcriptional analysis of recovered cells revealed only marginal alteration of their molecular profile upon DEP application, underscored by minor transcriptional changes induced upon injection into the microfluidic device. Therefore, the established microfluidic system combining targeted DEP manipulation with downstream hydrodynamic coordination of single cells provides a powerful means to handle and manipulate individual cells within one device. MDPI 2020-03-20 /pmc/articles/PMC7143322/ /pubmed/32244902 http://dx.doi.org/10.3390/mi11030322 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thiriet, Pierre-Emmanuel
Pezoldt, Joern
Gambardella, Gabriele
Keim, Kevin
Deplancke, Bart
Guiducci, Carlotta
Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow
title Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow
title_full Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow
title_fullStr Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow
title_full_unstemmed Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow
title_short Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow
title_sort selective retrieval of individual cells from microfluidic arrays combining dielectrophoretic force and directed hydrodynamic flow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143322/
https://www.ncbi.nlm.nih.gov/pubmed/32244902
http://dx.doi.org/10.3390/mi11030322
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