Cargando…
Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma
Semaphorins were discovered as guidance signals that mediate neural development. Recent studies suggest that semaphorin 3A (Sema3A), a member of the semaphorin family, is involved in the development of several cancers. This study aimed to analyze the association of Sema3A with the clinical features...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143379/ https://www.ncbi.nlm.nih.gov/pubmed/32192122 http://dx.doi.org/10.3390/microorganisms8030423 |
_version_ | 1783519598444806144 |
---|---|
author | Imoto, Tomoko Kondo, Satoru Wakisaka, Naohiro Hai, Pham Tahnh Seishima, Noriko Kano, Makoto Ueno, Takayoshi Mizokami, Harue Nakanishi, Yosuke Hatano, Miyako Endo, Kazuhira Sugimoto, Hisashi Moriyama-Kita, Makiko Yoshizaki, Tomokazu |
author_facet | Imoto, Tomoko Kondo, Satoru Wakisaka, Naohiro Hai, Pham Tahnh Seishima, Noriko Kano, Makoto Ueno, Takayoshi Mizokami, Harue Nakanishi, Yosuke Hatano, Miyako Endo, Kazuhira Sugimoto, Hisashi Moriyama-Kita, Makiko Yoshizaki, Tomokazu |
author_sort | Imoto, Tomoko |
collection | PubMed |
description | Semaphorins were discovered as guidance signals that mediate neural development. Recent studies suggest that semaphorin 3A (Sema3A), a member of the semaphorin family, is involved in the development of several cancers. This study aimed to analyze the association of Sema3A with the clinical features of nasopharyngeal carcinoma (NPC), an Epstein–Barr virus-associated carcinoma, and the Epstein–Barr virus primary oncogene latent membrane protein 1 (LMP1). The expression of Sema3A and LMP1 was immunohistochemically examined in the 35 NPC specimens. The mean expression scores for Sema3A and LMP1 were 20.8% ± 14.5% and 13.9% ± 14.8%, respectively. The expression of Sema3A significantly correlated with that of LMP1 (r = 0.41, p = 0.014). In addition, the Sema3A high cohort showed significantly poorer prognosis than the Sema3A low cohort. Sema3A expression was higher in the LMP1-positive KH-1 and KR-4 cell lines compared to the LMP1-negative HeLa cells. Overexpression of LMP1 in the LMP1-negative AdAH cell line upregulated Sema3A expression, both at the transcriptional and translational level. Finally, Sema3A expression was associated with poor prognosis in patients with NPC. Our data suggest that LMP1 induces the expression of Sema3A, which may promote tumor progression in NPC. |
format | Online Article Text |
id | pubmed-7143379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71433792020-04-14 Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma Imoto, Tomoko Kondo, Satoru Wakisaka, Naohiro Hai, Pham Tahnh Seishima, Noriko Kano, Makoto Ueno, Takayoshi Mizokami, Harue Nakanishi, Yosuke Hatano, Miyako Endo, Kazuhira Sugimoto, Hisashi Moriyama-Kita, Makiko Yoshizaki, Tomokazu Microorganisms Brief Report Semaphorins were discovered as guidance signals that mediate neural development. Recent studies suggest that semaphorin 3A (Sema3A), a member of the semaphorin family, is involved in the development of several cancers. This study aimed to analyze the association of Sema3A with the clinical features of nasopharyngeal carcinoma (NPC), an Epstein–Barr virus-associated carcinoma, and the Epstein–Barr virus primary oncogene latent membrane protein 1 (LMP1). The expression of Sema3A and LMP1 was immunohistochemically examined in the 35 NPC specimens. The mean expression scores for Sema3A and LMP1 were 20.8% ± 14.5% and 13.9% ± 14.8%, respectively. The expression of Sema3A significantly correlated with that of LMP1 (r = 0.41, p = 0.014). In addition, the Sema3A high cohort showed significantly poorer prognosis than the Sema3A low cohort. Sema3A expression was higher in the LMP1-positive KH-1 and KR-4 cell lines compared to the LMP1-negative HeLa cells. Overexpression of LMP1 in the LMP1-negative AdAH cell line upregulated Sema3A expression, both at the transcriptional and translational level. Finally, Sema3A expression was associated with poor prognosis in patients with NPC. Our data suggest that LMP1 induces the expression of Sema3A, which may promote tumor progression in NPC. MDPI 2020-03-17 /pmc/articles/PMC7143379/ /pubmed/32192122 http://dx.doi.org/10.3390/microorganisms8030423 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Imoto, Tomoko Kondo, Satoru Wakisaka, Naohiro Hai, Pham Tahnh Seishima, Noriko Kano, Makoto Ueno, Takayoshi Mizokami, Harue Nakanishi, Yosuke Hatano, Miyako Endo, Kazuhira Sugimoto, Hisashi Moriyama-Kita, Makiko Yoshizaki, Tomokazu Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
title | Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
title_full | Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
title_fullStr | Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
title_full_unstemmed | Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
title_short | Overexpression of Semaphorin 3A is a Marker Associated with Poor Prognosis in Patients with Nasopharyngeal Carcinoma |
title_sort | overexpression of semaphorin 3a is a marker associated with poor prognosis in patients with nasopharyngeal carcinoma |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143379/ https://www.ncbi.nlm.nih.gov/pubmed/32192122 http://dx.doi.org/10.3390/microorganisms8030423 |
work_keys_str_mv | AT imototomoko overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT kondosatoru overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT wakisakanaohiro overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT haiphamtahnh overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT seishimanoriko overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT kanomakoto overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT uenotakayoshi overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT mizokamiharue overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT nakanishiyosuke overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT hatanomiyako overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT endokazuhira overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT sugimotohisashi overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT moriyamakitamakiko overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma AT yoshizakitomokazu overexpressionofsemaphorin3aisamarkerassociatedwithpoorprognosisinpatientswithnasopharyngealcarcinoma |