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Cell Lysis Based on an Oscillating Microbubble Array
Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143388/ https://www.ncbi.nlm.nih.gov/pubmed/32164279 http://dx.doi.org/10.3390/mi11030288 |
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author | Liu, Xiufang Li, Jinyuan Zhang, Liangyu Huang, Xiaowei Farooq, Umar Pang, Na Zhou, Wei Qi, Lin Xu, Lisheng Niu, Lili Meng, Long |
author_facet | Liu, Xiufang Li, Jinyuan Zhang, Liangyu Huang, Xiaowei Farooq, Umar Pang, Na Zhou, Wei Qi, Lin Xu, Lisheng Niu, Lili Meng, Long |
author_sort | Liu, Xiufang |
collection | PubMed |
description | Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy. |
format | Online Article Text |
id | pubmed-7143388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71433882020-04-14 Cell Lysis Based on an Oscillating Microbubble Array Liu, Xiufang Li, Jinyuan Zhang, Liangyu Huang, Xiaowei Farooq, Umar Pang, Na Zhou, Wei Qi, Lin Xu, Lisheng Niu, Lili Meng, Long Micromachines (Basel) Article Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy. MDPI 2020-03-10 /pmc/articles/PMC7143388/ /pubmed/32164279 http://dx.doi.org/10.3390/mi11030288 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Xiufang Li, Jinyuan Zhang, Liangyu Huang, Xiaowei Farooq, Umar Pang, Na Zhou, Wei Qi, Lin Xu, Lisheng Niu, Lili Meng, Long Cell Lysis Based on an Oscillating Microbubble Array |
title | Cell Lysis Based on an Oscillating Microbubble Array |
title_full | Cell Lysis Based on an Oscillating Microbubble Array |
title_fullStr | Cell Lysis Based on an Oscillating Microbubble Array |
title_full_unstemmed | Cell Lysis Based on an Oscillating Microbubble Array |
title_short | Cell Lysis Based on an Oscillating Microbubble Array |
title_sort | cell lysis based on an oscillating microbubble array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143388/ https://www.ncbi.nlm.nih.gov/pubmed/32164279 http://dx.doi.org/10.3390/mi11030288 |
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