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Cell Lysis Based on an Oscillating Microbubble Array

Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble...

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Detalles Bibliográficos
Autores principales: Liu, Xiufang, Li, Jinyuan, Zhang, Liangyu, Huang, Xiaowei, Farooq, Umar, Pang, Na, Zhou, Wei, Qi, Lin, Xu, Lisheng, Niu, Lili, Meng, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143388/
https://www.ncbi.nlm.nih.gov/pubmed/32164279
http://dx.doi.org/10.3390/mi11030288
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author Liu, Xiufang
Li, Jinyuan
Zhang, Liangyu
Huang, Xiaowei
Farooq, Umar
Pang, Na
Zhou, Wei
Qi, Lin
Xu, Lisheng
Niu, Lili
Meng, Long
author_facet Liu, Xiufang
Li, Jinyuan
Zhang, Liangyu
Huang, Xiaowei
Farooq, Umar
Pang, Na
Zhou, Wei
Qi, Lin
Xu, Lisheng
Niu, Lili
Meng, Long
author_sort Liu, Xiufang
collection PubMed
description Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy.
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spelling pubmed-71433882020-04-14 Cell Lysis Based on an Oscillating Microbubble Array Liu, Xiufang Li, Jinyuan Zhang, Liangyu Huang, Xiaowei Farooq, Umar Pang, Na Zhou, Wei Qi, Lin Xu, Lisheng Niu, Lili Meng, Long Micromachines (Basel) Article Cell lysis is a process of breaking cell membranes to release intracellular substances such as DNA, RNA, protein, or organelles from a cell. The detection of DNA, RNA, or protein from the lysed cells is of importance for cancer diagnostics and drug screening. In this study, we develop a microbubble array that enables the realization of multiple cell lysis induced by the shear stress resulting from the individual oscillating microbubbles. The oscillating microbubbles in the channel have similar vibration amplitudes, and the intracellular substances can be released from the individual cells efficiently. Moreover, the efficiency of cell lysis increases with increments of input voltage and sonication time. By means of DNA agarose-gel electrophoresis, a sufficient extraction amount of DNA released from the lysed cells can be detected, and there is no significant difference in lysis efficiency when compared to cell lysis achieved using commercial kits. With the advantages of the simple manufacturing process, low cost, high efficiency, and high speed, this device can serve as an efficient and versatile tool for the single-cell sequencing of cell biology research, disease diagnosis, and stem cell therapy. MDPI 2020-03-10 /pmc/articles/PMC7143388/ /pubmed/32164279 http://dx.doi.org/10.3390/mi11030288 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xiufang
Li, Jinyuan
Zhang, Liangyu
Huang, Xiaowei
Farooq, Umar
Pang, Na
Zhou, Wei
Qi, Lin
Xu, Lisheng
Niu, Lili
Meng, Long
Cell Lysis Based on an Oscillating Microbubble Array
title Cell Lysis Based on an Oscillating Microbubble Array
title_full Cell Lysis Based on an Oscillating Microbubble Array
title_fullStr Cell Lysis Based on an Oscillating Microbubble Array
title_full_unstemmed Cell Lysis Based on an Oscillating Microbubble Array
title_short Cell Lysis Based on an Oscillating Microbubble Array
title_sort cell lysis based on an oscillating microbubble array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143388/
https://www.ncbi.nlm.nih.gov/pubmed/32164279
http://dx.doi.org/10.3390/mi11030288
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