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Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua
Foodborne infection with Listeria causes potentially life-threatening disease listeriosis. Listeria monocytogenes is widely recognized as the only species of public health concern, and the closely related species Listeria innocua is commonly used by the food industry as an indicator to identify envi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143663/ https://www.ncbi.nlm.nih.gov/pubmed/32183339 http://dx.doi.org/10.3390/microorganisms8030414 |
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author | Gwida, Mayada Lüth, Stefanie El-Ashker, Maged Zakaria, Amira El-Gohary, Fatma Elsayed, Mona Kleta, Sylvia Al Dahouk, Sascha |
author_facet | Gwida, Mayada Lüth, Stefanie El-Ashker, Maged Zakaria, Amira El-Gohary, Fatma Elsayed, Mona Kleta, Sylvia Al Dahouk, Sascha |
author_sort | Gwida, Mayada |
collection | PubMed |
description | Foodborne infection with Listeria causes potentially life-threatening disease listeriosis. Listeria monocytogenes is widely recognized as the only species of public health concern, and the closely related species Listeria innocua is commonly used by the food industry as an indicator to identify environmental conditions that allow for presence, growth, and persistence of Listeria spp. in general. In our study, we analyze the occurrence of Listeria spp. in a farm-to-fork approach in a poultry production chain in Egypt and identify bacterial entry gates and transmission systems. Prevalence of Listeria innocua at the three production stages (farm, slaughterhouse, food products) ranged from 11% to 28%. The pathogenic species Listeria monocytogenes was not detected, and Listeria innocua strains under study did not show genetic virulence determinants. However, the close genetic relatedness of Listeria innocua isolates (maximum 63 SNP differences) indicated cross-contamination between all stages from farm to final food product. Based on these results, chicken can be seen as a natural source of Listeria. Last but not least, sanitary measures during production should be reassessed to prevent bacterial contamination from entering the food chain and to consequently prevent human listeriosis infections. For this purpose, surveillance must not be restricted to pathogenic species. |
format | Online Article Text |
id | pubmed-7143663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71436632020-04-14 Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua Gwida, Mayada Lüth, Stefanie El-Ashker, Maged Zakaria, Amira El-Gohary, Fatma Elsayed, Mona Kleta, Sylvia Al Dahouk, Sascha Microorganisms Article Foodborne infection with Listeria causes potentially life-threatening disease listeriosis. Listeria monocytogenes is widely recognized as the only species of public health concern, and the closely related species Listeria innocua is commonly used by the food industry as an indicator to identify environmental conditions that allow for presence, growth, and persistence of Listeria spp. in general. In our study, we analyze the occurrence of Listeria spp. in a farm-to-fork approach in a poultry production chain in Egypt and identify bacterial entry gates and transmission systems. Prevalence of Listeria innocua at the three production stages (farm, slaughterhouse, food products) ranged from 11% to 28%. The pathogenic species Listeria monocytogenes was not detected, and Listeria innocua strains under study did not show genetic virulence determinants. However, the close genetic relatedness of Listeria innocua isolates (maximum 63 SNP differences) indicated cross-contamination between all stages from farm to final food product. Based on these results, chicken can be seen as a natural source of Listeria. Last but not least, sanitary measures during production should be reassessed to prevent bacterial contamination from entering the food chain and to consequently prevent human listeriosis infections. For this purpose, surveillance must not be restricted to pathogenic species. MDPI 2020-03-14 /pmc/articles/PMC7143663/ /pubmed/32183339 http://dx.doi.org/10.3390/microorganisms8030414 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gwida, Mayada Lüth, Stefanie El-Ashker, Maged Zakaria, Amira El-Gohary, Fatma Elsayed, Mona Kleta, Sylvia Al Dahouk, Sascha Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua |
title | Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua |
title_full | Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua |
title_fullStr | Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua |
title_full_unstemmed | Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua |
title_short | Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing of Listeria innocua |
title_sort | contamination pathways can be traced along the poultry processing chain by whole genome sequencing of listeria innocua |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143663/ https://www.ncbi.nlm.nih.gov/pubmed/32183339 http://dx.doi.org/10.3390/microorganisms8030414 |
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