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Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease

SIMPLE SUMMARY: Fatty liver disease is the most common liver abnormality, characterized by lipid accumulation within the hepatocytes. The disease results in a significant economic burden on the poultry industry. Microbial infection is recognized as a continuous challenge worldwide. The current study...

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Autores principales: Mei, Wenqing, Hao, Yarong, Xie, Huilin, Ni, Yingdong, Zhao, Ruqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143745/
https://www.ncbi.nlm.nih.gov/pubmed/32204385
http://dx.doi.org/10.3390/ani10030514
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author Mei, Wenqing
Hao, Yarong
Xie, Huilin
Ni, Yingdong
Zhao, Ruqian
author_facet Mei, Wenqing
Hao, Yarong
Xie, Huilin
Ni, Yingdong
Zhao, Ruqian
author_sort Mei, Wenqing
collection PubMed
description SIMPLE SUMMARY: Fatty liver disease is the most common liver abnormality, characterized by lipid accumulation within the hepatocytes. The disease results in a significant economic burden on the poultry industry. Microbial infection is recognized as a continuous challenge worldwide. The current study evaluated hepatic function and inflammatory response in broilers with fatty livers following acute lipopolysaccharide (LPS) challenge. Results indicate that broilers suffering from fatty liver disease are more susceptible to the negative effects of LPS, showing inflammatory response activation and more severe damage to the liver. ABSTRACT: This study aimed to examine hepatic function and inflammatory response in broilers with fatty livers, following acute lipopolysaccharide (LPS) challenge. One-day-old Lihua yellow broilers were fed a basal diet. Broilers were divided into four groups: control (CON), corticosterone treatment (CORT), LPS treatment (LPS), and LPS and CORT treatment (LPS&CORT). Results show that CORT induced an increase in plasma and liver triglycerides (TGs), which were accompanied by severe hepatic steatosis. The LPS group showed hepatocyte necrosis with inflammatory cell infiltration. Total liver damage score in the LPS&CORT group was significantly higher than that in the LPS group (p < 0.05). Activity levels of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were similar in the CON and CORT groups, but higher in the LPS group. Gene expression upregulation of the proinflammatory cytokines (NF-κB, IL-1β, IL-6, IFN-γ, and iNOS) was also noted in the LPS group (p < 0.05). In particular, LPS injection exacerbated the gene expression of these proinflammatory cytokines, even when accompanied by CORT injections (p < 0.05). In summary, our results indicate that broilers suffering from fatty liver disease are more susceptible to the negative effects of LPS, showing inflammatory response activation and more severe damages to the liver.
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spelling pubmed-71437452020-04-14 Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease Mei, Wenqing Hao, Yarong Xie, Huilin Ni, Yingdong Zhao, Ruqian Animals (Basel) Article SIMPLE SUMMARY: Fatty liver disease is the most common liver abnormality, characterized by lipid accumulation within the hepatocytes. The disease results in a significant economic burden on the poultry industry. Microbial infection is recognized as a continuous challenge worldwide. The current study evaluated hepatic function and inflammatory response in broilers with fatty livers following acute lipopolysaccharide (LPS) challenge. Results indicate that broilers suffering from fatty liver disease are more susceptible to the negative effects of LPS, showing inflammatory response activation and more severe damage to the liver. ABSTRACT: This study aimed to examine hepatic function and inflammatory response in broilers with fatty livers, following acute lipopolysaccharide (LPS) challenge. One-day-old Lihua yellow broilers were fed a basal diet. Broilers were divided into four groups: control (CON), corticosterone treatment (CORT), LPS treatment (LPS), and LPS and CORT treatment (LPS&CORT). Results show that CORT induced an increase in plasma and liver triglycerides (TGs), which were accompanied by severe hepatic steatosis. The LPS group showed hepatocyte necrosis with inflammatory cell infiltration. Total liver damage score in the LPS&CORT group was significantly higher than that in the LPS group (p < 0.05). Activity levels of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were similar in the CON and CORT groups, but higher in the LPS group. Gene expression upregulation of the proinflammatory cytokines (NF-κB, IL-1β, IL-6, IFN-γ, and iNOS) was also noted in the LPS group (p < 0.05). In particular, LPS injection exacerbated the gene expression of these proinflammatory cytokines, even when accompanied by CORT injections (p < 0.05). In summary, our results indicate that broilers suffering from fatty liver disease are more susceptible to the negative effects of LPS, showing inflammatory response activation and more severe damages to the liver. MDPI 2020-03-19 /pmc/articles/PMC7143745/ /pubmed/32204385 http://dx.doi.org/10.3390/ani10030514 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mei, Wenqing
Hao, Yarong
Xie, Huilin
Ni, Yingdong
Zhao, Ruqian
Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease
title Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease
title_full Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease
title_fullStr Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease
title_full_unstemmed Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease
title_short Hepatic Inflammatory Response to Exogenous LPS Challenge is Exacerbated in Broilers with Fatty Liver Disease
title_sort hepatic inflammatory response to exogenous lps challenge is exacerbated in broilers with fatty liver disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143745/
https://www.ncbi.nlm.nih.gov/pubmed/32204385
http://dx.doi.org/10.3390/ani10030514
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AT niyingdong hepaticinflammatoryresponsetoexogenouslpschallengeisexacerbatedinbroilerswithfattyliverdisease
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