Cargando…

Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)

In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many c...

Descripción completa

Detalles Bibliográficos
Autores principales: Navarro, Alejandro, Bárcena, Carmen, Pozo, Pilar, Díez-Guerrier, Alberto, Martínez, Irene, Polo, Coral, Duque, Clara, Rodríguez-Lázaro, David, Goyache, Joaquín, García, Nerea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144013/
https://www.ncbi.nlm.nih.gov/pubmed/32210090
http://dx.doi.org/10.3390/microorganisms8030450
_version_ 1783519749328601088
author Navarro, Alejandro
Bárcena, Carmen
Pozo, Pilar
Díez-Guerrier, Alberto
Martínez, Irene
Polo, Coral
Duque, Clara
Rodríguez-Lázaro, David
Goyache, Joaquín
García, Nerea
author_facet Navarro, Alejandro
Bárcena, Carmen
Pozo, Pilar
Díez-Guerrier, Alberto
Martínez, Irene
Polo, Coral
Duque, Clara
Rodríguez-Lázaro, David
Goyache, Joaquín
García, Nerea
author_sort Navarro, Alejandro
collection PubMed
description In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by ELISA in 68.0% of the serum samples, and in 61.6% of the undiluted transudate, and in 70.4%, 56.8%, and 44.8% of 1:2, 1:10, or 1:20 diluted transudate, respectively. The best results were obtained for the liver transudate at 1:10 dilution, based on the Kappa statistic (0.630) and intraclass correlation coefficient (0.841). HEV RNA was detected by RT-qPCR in 22.4% of the serum samples and 6.9% of the transudate samples, all samples used for RT-qPCR were positive by ELISA. Our results indicate that liver transudate may be an alternative matrix to serum for the detection of anti-HEV antibodies.
format Online
Article
Text
id pubmed-7144013
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71440132020-04-13 Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa) Navarro, Alejandro Bárcena, Carmen Pozo, Pilar Díez-Guerrier, Alberto Martínez, Irene Polo, Coral Duque, Clara Rodríguez-Lázaro, David Goyache, Joaquín García, Nerea Microorganisms Article In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by ELISA in 68.0% of the serum samples, and in 61.6% of the undiluted transudate, and in 70.4%, 56.8%, and 44.8% of 1:2, 1:10, or 1:20 diluted transudate, respectively. The best results were obtained for the liver transudate at 1:10 dilution, based on the Kappa statistic (0.630) and intraclass correlation coefficient (0.841). HEV RNA was detected by RT-qPCR in 22.4% of the serum samples and 6.9% of the transudate samples, all samples used for RT-qPCR were positive by ELISA. Our results indicate that liver transudate may be an alternative matrix to serum for the detection of anti-HEV antibodies. MDPI 2020-03-23 /pmc/articles/PMC7144013/ /pubmed/32210090 http://dx.doi.org/10.3390/microorganisms8030450 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Navarro, Alejandro
Bárcena, Carmen
Pozo, Pilar
Díez-Guerrier, Alberto
Martínez, Irene
Polo, Coral
Duque, Clara
Rodríguez-Lázaro, David
Goyache, Joaquín
García, Nerea
Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
title Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
title_full Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
title_fullStr Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
title_full_unstemmed Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
title_short Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
title_sort liver transudate, a potential alternative to detect anti-hepatitis e virus antibodies in pigs and wild boars (sus scrofa)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144013/
https://www.ncbi.nlm.nih.gov/pubmed/32210090
http://dx.doi.org/10.3390/microorganisms8030450
work_keys_str_mv AT navarroalejandro livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT barcenacarmen livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT pozopilar livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT diezguerrieralberto livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT martinezirene livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT polocoral livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT duqueclara livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT rodriguezlazarodavid livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT goyachejoaquin livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa
AT garcianerea livertransudateapotentialalternativetodetectantihepatitisevirusantibodiesinpigsandwildboarssusscrofa