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Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations
BACKGROUND: Evidence suggests that pathophysiological conditions such as obesity and type 2 diabetes (T2D) are associated with morphologic and metabolic alterations in the small intestinal mucosa. Exploring these alterations generally requires invasive methods, limiting data acquisition to subjects...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144049/ https://www.ncbi.nlm.nih.gov/pubmed/32292494 http://dx.doi.org/10.1186/s13098-020-00530-6 |
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author | Lalande, Catherine Drouin-Chartier, Jean-Philippe Tremblay, André J. Couture, Patrick Veilleux, Alain |
author_facet | Lalande, Catherine Drouin-Chartier, Jean-Philippe Tremblay, André J. Couture, Patrick Veilleux, Alain |
author_sort | Lalande, Catherine |
collection | PubMed |
description | BACKGROUND: Evidence suggests that pathophysiological conditions such as obesity and type 2 diabetes (T2D) are associated with morphologic and metabolic alterations in the small intestinal mucosa. Exploring these alterations generally requires invasive methods, limiting data acquisition to subjects with enteropathies or undergoing bariatric surgery. We aimed to evaluate small intestine epithelial cell homeostasis in a cohort of men covering a wide range of adiposity and glucose homoeostasis statuses. METHODS: Plasma levels of citrulline, a biomarker of enterocyte mass, and I-FABP, a biomarker of enterocyte death, were measured by UHPLC‑MS and ELISA in 154 nondiabetic men and 67 men with a T2D diagnosis. RESULTS: Plasma citrulline was significantly reduced in men with insulin resistance and T2D compared to insulin sensitive men. Decreased citrulline levels were, however, not observed in men with uncontrolled metabolic parameters during T2D. Plasma I-FABP was significantly higher in men with T2D, especially in presence of uncontrolled glycemic and lipid profile parameters. Integration of both parameters, which estimate enterocyte turnover, was associated with glucose homeostasis as well as with T2D diagnosis. Differences in biomarkers levels were independent of age and BMI and glucose filtration rates. CONCLUSIONS: Our study supports a decreased functional enterocyte mass and an increased enterocyte death rate in presence of metabolic alterations but emphasizes that epithelial cell homeostasis is especially altered in presence of severe insulin resistance and T2D. The marked changes in small intestine cellularity observed in obesity and diabetes are thus suggested to be part of gut dysfunctions, mainly at an advanced stage of the disease. |
format | Online Article Text |
id | pubmed-7144049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-71440492020-04-14 Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations Lalande, Catherine Drouin-Chartier, Jean-Philippe Tremblay, André J. Couture, Patrick Veilleux, Alain Diabetol Metab Syndr Research BACKGROUND: Evidence suggests that pathophysiological conditions such as obesity and type 2 diabetes (T2D) are associated with morphologic and metabolic alterations in the small intestinal mucosa. Exploring these alterations generally requires invasive methods, limiting data acquisition to subjects with enteropathies or undergoing bariatric surgery. We aimed to evaluate small intestine epithelial cell homeostasis in a cohort of men covering a wide range of adiposity and glucose homoeostasis statuses. METHODS: Plasma levels of citrulline, a biomarker of enterocyte mass, and I-FABP, a biomarker of enterocyte death, were measured by UHPLC‑MS and ELISA in 154 nondiabetic men and 67 men with a T2D diagnosis. RESULTS: Plasma citrulline was significantly reduced in men with insulin resistance and T2D compared to insulin sensitive men. Decreased citrulline levels were, however, not observed in men with uncontrolled metabolic parameters during T2D. Plasma I-FABP was significantly higher in men with T2D, especially in presence of uncontrolled glycemic and lipid profile parameters. Integration of both parameters, which estimate enterocyte turnover, was associated with glucose homeostasis as well as with T2D diagnosis. Differences in biomarkers levels were independent of age and BMI and glucose filtration rates. CONCLUSIONS: Our study supports a decreased functional enterocyte mass and an increased enterocyte death rate in presence of metabolic alterations but emphasizes that epithelial cell homeostasis is especially altered in presence of severe insulin resistance and T2D. The marked changes in small intestine cellularity observed in obesity and diabetes are thus suggested to be part of gut dysfunctions, mainly at an advanced stage of the disease. BioMed Central 2020-04-09 /pmc/articles/PMC7144049/ /pubmed/32292494 http://dx.doi.org/10.1186/s13098-020-00530-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Lalande, Catherine Drouin-Chartier, Jean-Philippe Tremblay, André J. Couture, Patrick Veilleux, Alain Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
title | Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
title_full | Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
title_fullStr | Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
title_full_unstemmed | Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
title_short | Plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
title_sort | plasma biomarkers of small intestine adaptations in obesity-related metabolic alterations |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144049/ https://www.ncbi.nlm.nih.gov/pubmed/32292494 http://dx.doi.org/10.1186/s13098-020-00530-6 |
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