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Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis

Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in...

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Autores principales: Lin, Tzu-Kai, Lin, Ya-Ping, Lin, Shun-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144089/
https://www.ncbi.nlm.nih.gov/pubmed/32046970
http://dx.doi.org/10.1534/g3.120.401091
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author Lin, Tzu-Kai
Lin, Ya-Ping
Lin, Shun-Fu
author_facet Lin, Tzu-Kai
Lin, Ya-Ping
Lin, Shun-Fu
author_sort Lin, Tzu-Kai
collection PubMed
description Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in B. rapa ssp. chinensis follows a three-allele model with BrMs(a), BrMs(b), and BrMs(c). The male sterility locus has been mapped to chromosome A07 in BC(1) and F(2) populations through genotyping by sequencing. Fine mapping in a total of 1,590 F(2) plants narrowed the male sterility gene BrMs to a 400 kb region, with two SNP markers only 0.3 cM from the gene. Comparative gene mapping shows that the Ms gene in B. rapa ssp. pekinensis is different from the BrMs gene of B. rapa ssp. chinensis, despite that both genes are located on chromosome A07. Interestingly, the DNA sequence orthologous to a male sterile gene in Brassica napus, BnRf, is within 400 kb of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one KASP marker (BrMs_indel) was developed for genotyping based on a 14 bp indel at intron 4. Cosegregation of male sterility and BrMs_indel genotypes in the F(2) population indicated that BnRf from B. napus and BrMs from B. rapa are likely to be orthologs. The BrMs_indel marker developed in this study will be useful in marker-assisted selection for the male sterility trait.
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spelling pubmed-71440892020-04-14 Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis Lin, Tzu-Kai Lin, Ya-Ping Lin, Shun-Fu G3 (Bethesda) Investigations Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in B. rapa ssp. chinensis follows a three-allele model with BrMs(a), BrMs(b), and BrMs(c). The male sterility locus has been mapped to chromosome A07 in BC(1) and F(2) populations through genotyping by sequencing. Fine mapping in a total of 1,590 F(2) plants narrowed the male sterility gene BrMs to a 400 kb region, with two SNP markers only 0.3 cM from the gene. Comparative gene mapping shows that the Ms gene in B. rapa ssp. pekinensis is different from the BrMs gene of B. rapa ssp. chinensis, despite that both genes are located on chromosome A07. Interestingly, the DNA sequence orthologous to a male sterile gene in Brassica napus, BnRf, is within 400 kb of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one KASP marker (BrMs_indel) was developed for genotyping based on a 14 bp indel at intron 4. Cosegregation of male sterility and BrMs_indel genotypes in the F(2) population indicated that BnRf from B. napus and BrMs from B. rapa are likely to be orthologs. The BrMs_indel marker developed in this study will be useful in marker-assisted selection for the male sterility trait. Genetics Society of America 2020-02-11 /pmc/articles/PMC7144089/ /pubmed/32046970 http://dx.doi.org/10.1534/g3.120.401091 Text en Copyright © 2020 Lin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Lin, Tzu-Kai
Lin, Ya-Ping
Lin, Shun-Fu
Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis
title Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis
title_full Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis
title_fullStr Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis
title_full_unstemmed Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis
title_short Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene in Brassica rapa ssp. chinensis
title_sort genetic analysis and fine mapping of a spontaneously mutated male sterility gene in brassica rapa ssp. chinensis
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144089/
https://www.ncbi.nlm.nih.gov/pubmed/32046970
http://dx.doi.org/10.1534/g3.120.401091
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