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Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction

Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds f...

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Autores principales: Nuapia, Yannick, Tutu, Hlanganani, Chimuka, Luke, Cukrowska, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144126/
https://www.ncbi.nlm.nih.gov/pubmed/32183432
http://dx.doi.org/10.3390/molecules25061335
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author Nuapia, Yannick
Tutu, Hlanganani
Chimuka, Luke
Cukrowska, Ewa
author_facet Nuapia, Yannick
Tutu, Hlanganani
Chimuka, Luke
Cukrowska, Ewa
author_sort Nuapia, Yannick
collection PubMed
description Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5–60 min), extraction temperature (50–200 °C) and collector vessel temperature (25–200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector′s vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly.
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spelling pubmed-71441262020-04-13 Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction Nuapia, Yannick Tutu, Hlanganani Chimuka, Luke Cukrowska, Ewa Molecules Article Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5–60 min), extraction temperature (50–200 °C) and collector vessel temperature (25–200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector′s vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly. MDPI 2020-03-15 /pmc/articles/PMC7144126/ /pubmed/32183432 http://dx.doi.org/10.3390/molecules25061335 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nuapia, Yannick
Tutu, Hlanganani
Chimuka, Luke
Cukrowska, Ewa
Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction
title Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction
title_full Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction
title_fullStr Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction
title_full_unstemmed Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction
title_short Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction
title_sort selective extraction of cannabinoid compounds from cannabis seed using pressurized hot water extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144126/
https://www.ncbi.nlm.nih.gov/pubmed/32183432
http://dx.doi.org/10.3390/molecules25061335
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