Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles

[Image: see text] In this work, the preparation of novel calcium citrate (CaCit) nanoparticles (NPs) has been disclosed and the use of these NPs as “Trojan” carriers has been demonstrated. The concentration ratio between calcium ions and citrate ions was optimized, yielding spherical NPs with size i...

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Autores principales: Rimsueb, Natchanon, Cherdchom, Sarocha, Aksornkitti, Vitavat, Khotavivattana, Tanatorn, Sereemaspun, Amornpun, Rojanathanes, Rojrit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144169/
https://www.ncbi.nlm.nih.gov/pubmed/32280883
http://dx.doi.org/10.1021/acsomega.0c00032
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author Rimsueb, Natchanon
Cherdchom, Sarocha
Aksornkitti, Vitavat
Khotavivattana, Tanatorn
Sereemaspun, Amornpun
Rojanathanes, Rojrit
author_facet Rimsueb, Natchanon
Cherdchom, Sarocha
Aksornkitti, Vitavat
Khotavivattana, Tanatorn
Sereemaspun, Amornpun
Rojanathanes, Rojrit
author_sort Rimsueb, Natchanon
collection PubMed
description [Image: see text] In this work, the preparation of novel calcium citrate (CaCit) nanoparticles (NPs) has been disclosed and the use of these NPs as “Trojan” carriers has been demonstrated. The concentration ratio between calcium ions and citrate ions was optimized, yielding spherical NPs with size in the range of 100–200 nm. Additionally, a fluorescent dye, fluorescein isothiocyanate (FITC), was successfully encapsulated by the coprecipitation method. The products were characterized by thermogravimetric analysis and scanning electron microscopy. The cellular uptake was investigated by incubating the synthesized fluorescent-tagged NPs with human keratinocytes using a confocal microscope. The accumulation of the FITC in the cells suggested that the CaCit NPs can potentially be used as novel drug carriers.
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spelling pubmed-71441692020-04-10 Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles Rimsueb, Natchanon Cherdchom, Sarocha Aksornkitti, Vitavat Khotavivattana, Tanatorn Sereemaspun, Amornpun Rojanathanes, Rojrit ACS Omega [Image: see text] In this work, the preparation of novel calcium citrate (CaCit) nanoparticles (NPs) has been disclosed and the use of these NPs as “Trojan” carriers has been demonstrated. The concentration ratio between calcium ions and citrate ions was optimized, yielding spherical NPs with size in the range of 100–200 nm. Additionally, a fluorescent dye, fluorescein isothiocyanate (FITC), was successfully encapsulated by the coprecipitation method. The products were characterized by thermogravimetric analysis and scanning electron microscopy. The cellular uptake was investigated by incubating the synthesized fluorescent-tagged NPs with human keratinocytes using a confocal microscope. The accumulation of the FITC in the cells suggested that the CaCit NPs can potentially be used as novel drug carriers. American Chemical Society 2020-03-27 /pmc/articles/PMC7144169/ /pubmed/32280883 http://dx.doi.org/10.1021/acsomega.0c00032 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Rimsueb, Natchanon
Cherdchom, Sarocha
Aksornkitti, Vitavat
Khotavivattana, Tanatorn
Sereemaspun, Amornpun
Rojanathanes, Rojrit
Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles
title Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles
title_full Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles
title_fullStr Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles
title_full_unstemmed Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles
title_short Feeding Cells with a Novel “Trojan” Carrier: Citrate Nanoparticles
title_sort feeding cells with a novel “trojan” carrier: citrate nanoparticles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144169/
https://www.ncbi.nlm.nih.gov/pubmed/32280883
http://dx.doi.org/10.1021/acsomega.0c00032
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