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Resveratrol inhibits ACHN cells via regulation of histone acetylation

CONTEXT: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. OBJECTIVE: To explore the functional role of resveratrol in RCC. MATERIALS AND METHODS: Functional experiments were performed to determine proliferatio n of ACHN cells with...

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Autores principales: Dai, Lili, Chen, Lingyan, Wang, Wenjing, Lin, Peizheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144206/
https://www.ncbi.nlm.nih.gov/pubmed/32202448
http://dx.doi.org/10.1080/13880209.2020.1738503
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author Dai, Lili
Chen, Lingyan
Wang, Wenjing
Lin, Peizheng
author_facet Dai, Lili
Chen, Lingyan
Wang, Wenjing
Lin, Peizheng
author_sort Dai, Lili
collection PubMed
description CONTEXT: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. OBJECTIVE: To explore the functional role of resveratrol in RCC. MATERIALS AND METHODS: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. RESULTS: When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation (p < 0.01) was increased by resveratrol. DISCUSSION AND CONCLUSIONS: Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC.
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spelling pubmed-71442062020-04-13 Resveratrol inhibits ACHN cells via regulation of histone acetylation Dai, Lili Chen, Lingyan Wang, Wenjing Lin, Peizheng Pharm Biol Research Article CONTEXT: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. OBJECTIVE: To explore the functional role of resveratrol in RCC. MATERIALS AND METHODS: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. RESULTS: When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation (p < 0.01) was increased by resveratrol. DISCUSSION AND CONCLUSIONS: Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC. Taylor & Francis 2020-03-23 /pmc/articles/PMC7144206/ /pubmed/32202448 http://dx.doi.org/10.1080/13880209.2020.1738503 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dai, Lili
Chen, Lingyan
Wang, Wenjing
Lin, Peizheng
Resveratrol inhibits ACHN cells via regulation of histone acetylation
title Resveratrol inhibits ACHN cells via regulation of histone acetylation
title_full Resveratrol inhibits ACHN cells via regulation of histone acetylation
title_fullStr Resveratrol inhibits ACHN cells via regulation of histone acetylation
title_full_unstemmed Resveratrol inhibits ACHN cells via regulation of histone acetylation
title_short Resveratrol inhibits ACHN cells via regulation of histone acetylation
title_sort resveratrol inhibits achn cells via regulation of histone acetylation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144206/
https://www.ncbi.nlm.nih.gov/pubmed/32202448
http://dx.doi.org/10.1080/13880209.2020.1738503
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