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Resveratrol inhibits ACHN cells via regulation of histone acetylation
CONTEXT: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. OBJECTIVE: To explore the functional role of resveratrol in RCC. MATERIALS AND METHODS: Functional experiments were performed to determine proliferatio n of ACHN cells with...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144206/ https://www.ncbi.nlm.nih.gov/pubmed/32202448 http://dx.doi.org/10.1080/13880209.2020.1738503 |
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author | Dai, Lili Chen, Lingyan Wang, Wenjing Lin, Peizheng |
author_facet | Dai, Lili Chen, Lingyan Wang, Wenjing Lin, Peizheng |
author_sort | Dai, Lili |
collection | PubMed |
description | CONTEXT: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. OBJECTIVE: To explore the functional role of resveratrol in RCC. MATERIALS AND METHODS: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. RESULTS: When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation (p < 0.01) was increased by resveratrol. DISCUSSION AND CONCLUSIONS: Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC. |
format | Online Article Text |
id | pubmed-7144206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-71442062020-04-13 Resveratrol inhibits ACHN cells via regulation of histone acetylation Dai, Lili Chen, Lingyan Wang, Wenjing Lin, Peizheng Pharm Biol Research Article CONTEXT: The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. OBJECTIVE: To explore the functional role of resveratrol in RCC. MATERIALS AND METHODS: Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. RESULTS: When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation (p < 0.01) was increased by resveratrol. DISCUSSION AND CONCLUSIONS: Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC. Taylor & Francis 2020-03-23 /pmc/articles/PMC7144206/ /pubmed/32202448 http://dx.doi.org/10.1080/13880209.2020.1738503 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dai, Lili Chen, Lingyan Wang, Wenjing Lin, Peizheng Resveratrol inhibits ACHN cells via regulation of histone acetylation |
title | Resveratrol inhibits ACHN cells via regulation of histone acetylation |
title_full | Resveratrol inhibits ACHN cells via regulation of histone acetylation |
title_fullStr | Resveratrol inhibits ACHN cells via regulation of histone acetylation |
title_full_unstemmed | Resveratrol inhibits ACHN cells via regulation of histone acetylation |
title_short | Resveratrol inhibits ACHN cells via regulation of histone acetylation |
title_sort | resveratrol inhibits achn cells via regulation of histone acetylation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144206/ https://www.ncbi.nlm.nih.gov/pubmed/32202448 http://dx.doi.org/10.1080/13880209.2020.1738503 |
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