Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P–S Bonded Organophosphorus as Monitored by Spectrofluorimetry

Enzyme-catalyzed hydrolysis of echothiophate, a P–S bonded organophosphorus (OP) model, was spectrofluorimetrically monitored, using Calbiochem Probe IV as the thiol reagent. OP hydrolases were: the G117H mutant of human butyrylcholinesterase capable of hydrolyzing OPs, and a multiple mutant of Brev...

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Detalles Bibliográficos
Autores principales: Zueva, Irina V., Lushchekina, Sofya V., Daudé, David, Chabrière, Eric, Masson, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144395/
https://www.ncbi.nlm.nih.gov/pubmed/32192230
http://dx.doi.org/10.3390/molecules25061371

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144395/
https://www.ncbi.nlm.nih.gov/pubmed/32192230
http://dx.doi.org/10.3390/molecules25061371

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