Cargando…

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of Zn...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahmad, Muhammad Arslan, Javed, Rabia, Adeel, Muhammad, Rizwan, Muhammad, Ao, Qiang, Yang, Yuesuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144565/
https://www.ncbi.nlm.nih.gov/pubmed/32192031
http://dx.doi.org/10.3390/molecules25061356
_version_ 1783519859640893440
author Ahmad, Muhammad Arslan
Javed, Rabia
Adeel, Muhammad
Rizwan, Muhammad
Ao, Qiang
Yang, Yuesuo
author_facet Ahmad, Muhammad Arslan
Javed, Rabia
Adeel, Muhammad
Rizwan, Muhammad
Ao, Qiang
Yang, Yuesuo
author_sort Ahmad, Muhammad Arslan
collection PubMed
description Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, Stevia rebaudiana. ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact.
format Online
Article
Text
id pubmed-7144565
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71445652020-04-15 Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana) Ahmad, Muhammad Arslan Javed, Rabia Adeel, Muhammad Rizwan, Muhammad Ao, Qiang Yang, Yuesuo Molecules Article Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, Stevia rebaudiana. ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact. MDPI 2020-03-17 /pmc/articles/PMC7144565/ /pubmed/32192031 http://dx.doi.org/10.3390/molecules25061356 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmad, Muhammad Arslan
Javed, Rabia
Adeel, Muhammad
Rizwan, Muhammad
Ao, Qiang
Yang, Yuesuo
Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)
title Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)
title_full Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)
title_fullStr Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)
title_full_unstemmed Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)
title_short Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)
title_sort engineered zno and cuo nanoparticles ameliorate morphological and biochemical response in tissue culture regenerants of candyleaf (stevia rebaudiana)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144565/
https://www.ncbi.nlm.nih.gov/pubmed/32192031
http://dx.doi.org/10.3390/molecules25061356
work_keys_str_mv AT ahmadmuhammadarslan engineeredznoandcuonanoparticlesamelioratemorphologicalandbiochemicalresponseintissuecultureregenerantsofcandyleafsteviarebaudiana
AT javedrabia engineeredznoandcuonanoparticlesamelioratemorphologicalandbiochemicalresponseintissuecultureregenerantsofcandyleafsteviarebaudiana
AT adeelmuhammad engineeredznoandcuonanoparticlesamelioratemorphologicalandbiochemicalresponseintissuecultureregenerantsofcandyleafsteviarebaudiana
AT rizwanmuhammad engineeredznoandcuonanoparticlesamelioratemorphologicalandbiochemicalresponseintissuecultureregenerantsofcandyleafsteviarebaudiana
AT aoqiang engineeredznoandcuonanoparticlesamelioratemorphologicalandbiochemicalresponseintissuecultureregenerantsofcandyleafsteviarebaudiana
AT yangyuesuo engineeredznoandcuonanoparticlesamelioratemorphologicalandbiochemicalresponseintissuecultureregenerantsofcandyleafsteviarebaudiana