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Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors
In analogy to split-protein systems, which rely on the appropriate fragmentation of protein domains, split aptamers made of two or more short nucleic acid strands have emerged as novel tools in biosensor set-ups. The concept relies on dissecting an aptamer into a series of two or more independent fr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144939/ https://www.ncbi.nlm.nih.gov/pubmed/32112111 http://dx.doi.org/10.1093/nar/gkaa132 |
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author | Debiais, Mégane Lelievre, Amandine Smietana, Michael Müller, Sabine |
author_facet | Debiais, Mégane Lelievre, Amandine Smietana, Michael Müller, Sabine |
author_sort | Debiais, Mégane |
collection | PubMed |
description | In analogy to split-protein systems, which rely on the appropriate fragmentation of protein domains, split aptamers made of two or more short nucleic acid strands have emerged as novel tools in biosensor set-ups. The concept relies on dissecting an aptamer into a series of two or more independent fragments, able to assemble in the presence of a specific target. The stability of the assembled structure can further be enhanced by functionalities that upon folding would lead to covalent end-joining of the fragments. To date, only a few aptamers have been split successfully, and application of split aptamers in biosensing approaches remains as promising as it is challenging. Further improving the stability of split aptamer target complexes and with that the sensitivity as well as efficient working modes are important tasks. Here we review functional nucleic acid assemblies that are derived from aptamers and ribozymes/DNAzymes. We focus on the thrombin, the adenosine/ATP and the cocaine split aptamers as the three most studied DNA split systems and on split DNAzyme assemblies. Furthermore, we extend the subject into split light up RNA aptamers used as mimics of the green fluorescent protein (GFP), and split ribozymes. |
format | Online Article Text |
id | pubmed-7144939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-71449392020-04-13 Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors Debiais, Mégane Lelievre, Amandine Smietana, Michael Müller, Sabine Nucleic Acids Res Survey and Summary In analogy to split-protein systems, which rely on the appropriate fragmentation of protein domains, split aptamers made of two or more short nucleic acid strands have emerged as novel tools in biosensor set-ups. The concept relies on dissecting an aptamer into a series of two or more independent fragments, able to assemble in the presence of a specific target. The stability of the assembled structure can further be enhanced by functionalities that upon folding would lead to covalent end-joining of the fragments. To date, only a few aptamers have been split successfully, and application of split aptamers in biosensing approaches remains as promising as it is challenging. Further improving the stability of split aptamer target complexes and with that the sensitivity as well as efficient working modes are important tasks. Here we review functional nucleic acid assemblies that are derived from aptamers and ribozymes/DNAzymes. We focus on the thrombin, the adenosine/ATP and the cocaine split aptamers as the three most studied DNA split systems and on split DNAzyme assemblies. Furthermore, we extend the subject into split light up RNA aptamers used as mimics of the green fluorescent protein (GFP), and split ribozymes. Oxford University Press 2020-04-17 2020-02-29 /pmc/articles/PMC7144939/ /pubmed/32112111 http://dx.doi.org/10.1093/nar/gkaa132 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Survey and Summary Debiais, Mégane Lelievre, Amandine Smietana, Michael Müller, Sabine Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
title | Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
title_full | Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
title_fullStr | Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
title_full_unstemmed | Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
title_short | Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
title_sort | splitting aptamers and nucleic acid enzymes for the development of advanced biosensors |
topic | Survey and Summary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144939/ https://www.ncbi.nlm.nih.gov/pubmed/32112111 http://dx.doi.org/10.1093/nar/gkaa132 |
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