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A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina

PURPOSE: To investigate the longitudinal findings of spectral-domain optical coherence tomography (SD-OCT) in relation to the morphologic features in Rdh5 knockout (Rdh5(-/-)) mice. MATERIALS AND METHODS: The mouse retina was segmented into four layers; the inner retinal (A), outer plexiform and out...

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Autores principales: Xie, Yuting, Gonome, Takayuki, Yamauchi, Kodai, Maeda-Monai, Natsuki, Tanabu, Reiko, Ishiguro, Sei-ichi, Nakazawa, Mitsuru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144952/
https://www.ncbi.nlm.nih.gov/pubmed/32271812
http://dx.doi.org/10.1371/journal.pone.0231220
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author Xie, Yuting
Gonome, Takayuki
Yamauchi, Kodai
Maeda-Monai, Natsuki
Tanabu, Reiko
Ishiguro, Sei-ichi
Nakazawa, Mitsuru
author_facet Xie, Yuting
Gonome, Takayuki
Yamauchi, Kodai
Maeda-Monai, Natsuki
Tanabu, Reiko
Ishiguro, Sei-ichi
Nakazawa, Mitsuru
author_sort Xie, Yuting
collection PubMed
description PURPOSE: To investigate the longitudinal findings of spectral-domain optical coherence tomography (SD-OCT) in relation to the morphologic features in Rdh5 knockout (Rdh5(-/-)) mice. MATERIALS AND METHODS: The mouse retina was segmented into four layers; the inner retinal (A), outer plexiform and outer nuclear (B), rod/cone (C), and retinal pigment epithelium (RPE)/choroid (D) layers. The thickness of each retinal layer of Rdh5(-/-) mice was longitudinally and quantitatively measured at six time points from postnatal months (PM) 1 to PM6 using SD-OCT. Age-matched C57BL/6J mice were employed as wild-type controls. The data were statistically compared using Student’s t-test. The fundus appearance was assessed, histologic and ultrastructural examinations were performed in both groups. RESULTS: Layers A and B were significantly thinner in the Rdh5(-/-) mice than in the wild-type C57BL/6J mice during the observation periods. Layers C and D became thinner in the Rdh5(-/-) mice than in the wild-type mice after PM6. Although no abnormalities corresponding to whitish fundus dots were detected by SD-OCT or histologic examinations, the intracellular accumulation of low-density vacuoles was noted in the RPE of the Rdh5(-/-) mice by electron microscopy. The photoreceptor nuclei appeared less dense in the Rdh5(-/-) mice than in the wild-type mice. DISCUSSION: The results from the present study suggest that although it is difficult to detect qualitative abnormalities, SD-OCT can detect quantitative changes in photoreceptors even in the early stage of retinal degeneration induced by the Rdh5 gene mutation in mice.
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spelling pubmed-71449522020-04-10 A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina Xie, Yuting Gonome, Takayuki Yamauchi, Kodai Maeda-Monai, Natsuki Tanabu, Reiko Ishiguro, Sei-ichi Nakazawa, Mitsuru PLoS One Research Article PURPOSE: To investigate the longitudinal findings of spectral-domain optical coherence tomography (SD-OCT) in relation to the morphologic features in Rdh5 knockout (Rdh5(-/-)) mice. MATERIALS AND METHODS: The mouse retina was segmented into four layers; the inner retinal (A), outer plexiform and outer nuclear (B), rod/cone (C), and retinal pigment epithelium (RPE)/choroid (D) layers. The thickness of each retinal layer of Rdh5(-/-) mice was longitudinally and quantitatively measured at six time points from postnatal months (PM) 1 to PM6 using SD-OCT. Age-matched C57BL/6J mice were employed as wild-type controls. The data were statistically compared using Student’s t-test. The fundus appearance was assessed, histologic and ultrastructural examinations were performed in both groups. RESULTS: Layers A and B were significantly thinner in the Rdh5(-/-) mice than in the wild-type C57BL/6J mice during the observation periods. Layers C and D became thinner in the Rdh5(-/-) mice than in the wild-type mice after PM6. Although no abnormalities corresponding to whitish fundus dots were detected by SD-OCT or histologic examinations, the intracellular accumulation of low-density vacuoles was noted in the RPE of the Rdh5(-/-) mice by electron microscopy. The photoreceptor nuclei appeared less dense in the Rdh5(-/-) mice than in the wild-type mice. DISCUSSION: The results from the present study suggest that although it is difficult to detect qualitative abnormalities, SD-OCT can detect quantitative changes in photoreceptors even in the early stage of retinal degeneration induced by the Rdh5 gene mutation in mice. Public Library of Science 2020-04-09 /pmc/articles/PMC7144952/ /pubmed/32271812 http://dx.doi.org/10.1371/journal.pone.0231220 Text en © 2020 Xie et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xie, Yuting
Gonome, Takayuki
Yamauchi, Kodai
Maeda-Monai, Natsuki
Tanabu, Reiko
Ishiguro, Sei-ichi
Nakazawa, Mitsuru
A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina
title A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina
title_full A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina
title_fullStr A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina
title_full_unstemmed A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina
title_short A spectral-domain optical coherence tomographic analysis of Rdh5(-/-) mice retina
title_sort spectral-domain optical coherence tomographic analysis of rdh5(-/-) mice retina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144952/
https://www.ncbi.nlm.nih.gov/pubmed/32271812
http://dx.doi.org/10.1371/journal.pone.0231220
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