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Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase

A flavone luteolin has various health-promoting activities. Several studies reported that high dose of luteolin activates the Nrf2/ARE pathway in the liver. However, the effect of the low dose of luteolin that can be taken from a dietary meal on the Nrf2 activation remain unclear. It is expected tha...

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Autores principales: Kitakaze, Tomoya, Makiyama, Atsushi, Yamashita, Yoko, Ashida, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144991/
https://www.ncbi.nlm.nih.gov/pubmed/32271832
http://dx.doi.org/10.1371/journal.pone.0231403
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author Kitakaze, Tomoya
Makiyama, Atsushi
Yamashita, Yoko
Ashida, Hitoshi
author_facet Kitakaze, Tomoya
Makiyama, Atsushi
Yamashita, Yoko
Ashida, Hitoshi
author_sort Kitakaze, Tomoya
collection PubMed
description A flavone luteolin has various health-promoting activities. Several studies reported that high dose of luteolin activates the Nrf2/ARE pathway in the liver. However, the effect of the low dose of luteolin that can be taken from a dietary meal on the Nrf2 activation remain unclear. It is expected that the flavonoid metabolism possesses a circadian rhythm, since nutritional metabolism processes daily cycle. In this study we investigated whether an administration affects the Nrf2 activation. ICR mice were orally administered 0.01–10 mg/kg body weight of luteolin once a day for 7 days at two time-points: at the start of active phase (ZT12) or at that of inactive phase (ZT0). Luteolin increased the nuclear translocation of Nrf2, resulting in the increases in its target gene products HO-1 and NQO1 at ZT12 but not at ZT0. The expression level of Nrf2 was lower at ZT12 than at ZT0 in the liver. We also found that the level of luteolin aglycon in the plasma is higher at ZT12 than at ZT0. These results suggest that the low dose of luteolin can activate Nrf2 pathway and the aglycon form of luteolin may mainly contribute to activate the Nrf2 pathway at ZT12 in the liver.
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spelling pubmed-71449912020-04-14 Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase Kitakaze, Tomoya Makiyama, Atsushi Yamashita, Yoko Ashida, Hitoshi PLoS One Research Article A flavone luteolin has various health-promoting activities. Several studies reported that high dose of luteolin activates the Nrf2/ARE pathway in the liver. However, the effect of the low dose of luteolin that can be taken from a dietary meal on the Nrf2 activation remain unclear. It is expected that the flavonoid metabolism possesses a circadian rhythm, since nutritional metabolism processes daily cycle. In this study we investigated whether an administration affects the Nrf2 activation. ICR mice were orally administered 0.01–10 mg/kg body weight of luteolin once a day for 7 days at two time-points: at the start of active phase (ZT12) or at that of inactive phase (ZT0). Luteolin increased the nuclear translocation of Nrf2, resulting in the increases in its target gene products HO-1 and NQO1 at ZT12 but not at ZT0. The expression level of Nrf2 was lower at ZT12 than at ZT0 in the liver. We also found that the level of luteolin aglycon in the plasma is higher at ZT12 than at ZT0. These results suggest that the low dose of luteolin can activate Nrf2 pathway and the aglycon form of luteolin may mainly contribute to activate the Nrf2 pathway at ZT12 in the liver. Public Library of Science 2020-04-09 /pmc/articles/PMC7144991/ /pubmed/32271832 http://dx.doi.org/10.1371/journal.pone.0231403 Text en © 2020 Kitakaze et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kitakaze, Tomoya
Makiyama, Atsushi
Yamashita, Yoko
Ashida, Hitoshi
Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
title Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
title_full Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
title_fullStr Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
title_full_unstemmed Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
title_short Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
title_sort low dose of luteolin activates nrf2 in the liver of mice at start of the active phase but not that of the inactive phase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144991/
https://www.ncbi.nlm.nih.gov/pubmed/32271832
http://dx.doi.org/10.1371/journal.pone.0231403
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