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Associations of dicarbonyl stress with complement activation: the CODAM study

AIMS/HYPOTHESIS: Reactive α-dicarbonyl compounds are major precursors of AGEs and may lead to glycation of circulating and/or cell-associated complement regulators. Glycation of complement regulatory proteins can influence their capacity to inhibit complement activation. We investigated, in a human...

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Autores principales: Xin, Ying, Hertle, Elisabeth, van der Kallen, Carla J. H., Schalkwijk, Casper G., Stehouwer, Coen D. A., van Greevenbroek, Marleen M. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145776/
https://www.ncbi.nlm.nih.gov/pubmed/31993713
http://dx.doi.org/10.1007/s00125-020-05098-4
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author Xin, Ying
Hertle, Elisabeth
van der Kallen, Carla J. H.
Schalkwijk, Casper G.
Stehouwer, Coen D. A.
van Greevenbroek, Marleen M. J.
author_facet Xin, Ying
Hertle, Elisabeth
van der Kallen, Carla J. H.
Schalkwijk, Casper G.
Stehouwer, Coen D. A.
van Greevenbroek, Marleen M. J.
author_sort Xin, Ying
collection PubMed
description AIMS/HYPOTHESIS: Reactive α-dicarbonyl compounds are major precursors of AGEs and may lead to glycation of circulating and/or cell-associated complement regulators. Glycation of complement regulatory proteins can influence their capacity to inhibit complement activation. We investigated, in a human cohort, whether greater dicarbonyl stress was associated with more complement activation. METHODS: Circulating concentrations of dicarbonyl stress markers, i.e. α-dicarbonyls (methylglyoxal [MGO], glyoxal [GO] and 3-deoxyglucosone [3-DG]), and free AGEs (N(ε)-(carboxymethyl)lysine [CML], N(ε)-(carboxyethyl)lysine [CEL] and N(δ)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine [MG-H1]), and protein-bound AGEs (CML, CEL, pentosidine), as well as the complement activation products C3a and soluble C5b-9 (sC5b-9), were measured in 530 participants (59.5 ± 7.0 years [mean ± SD], 61% men) of the Cohort on Diabetes and Atherosclerosis Maastricht (CODAM) study. Multiple linear regression analyses were used to investigate the associations between dicarbonyl stress (standardised) and complement activation (standardised) with adjustment of potential confounders, including age, sex, lifestyle, use of medication and markers of obesity. In addition, the associations of two potentially functional polymorphisms (rs1049346, rs2736654) in the gene encoding glyoxalase 1 (GLO1), the rate-limiting detoxifying enzyme for MGO, with C3a and sC5b-9 (all standardized) were evaluated. RESULTS: After adjustment for potential confounders, plasma concentration of the dicarbonyl GO was inversely associated with sC5b-9 (β −0.12 [95% CI –0.21, −0.02]) and the protein-bound AGE CEL was inversely associated with C3a (−0.17 [−0.25, −0.08]). In contrast, the protein-bound AGE pentosidine was positively associated with sC5b-9 (0.15 [0.05, 0.24]). No associations were observed for other α-dicarbonyls and other free or protein-bound AGEs with C3a or sC5b-9. Individuals with the AG and AA genotype of rs1049346 had, on average, 0.32 and 0.40 SD lower plasma concentrations of sC5b-9 than those with the GG genotype, while concentrations of C3a did not differ significantly between rs1049346 genotypes. GLO1 rs2736654 was not associated with either C3a or sC5b-9. CONCLUSIONS/INTERPRETATION: Plasma concentrations of dicarbonyl stress markers showed distinct associations with complement activation products: some of them were inversely associated with either C3a or sC5b-9, while protein-bound pentosidine was consistently and positively associated with sC5b-9. This suggests different biological relationships of individual dicarbonyl stress markers with complement activation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00125-020-05098-4) contains peer-reviewed but unedited supplementary material, which is available to authorised users.
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spelling pubmed-71457762020-04-15 Associations of dicarbonyl stress with complement activation: the CODAM study Xin, Ying Hertle, Elisabeth van der Kallen, Carla J. H. Schalkwijk, Casper G. Stehouwer, Coen D. A. van Greevenbroek, Marleen M. J. Diabetologia Article AIMS/HYPOTHESIS: Reactive α-dicarbonyl compounds are major precursors of AGEs and may lead to glycation of circulating and/or cell-associated complement regulators. Glycation of complement regulatory proteins can influence their capacity to inhibit complement activation. We investigated, in a human cohort, whether greater dicarbonyl stress was associated with more complement activation. METHODS: Circulating concentrations of dicarbonyl stress markers, i.e. α-dicarbonyls (methylglyoxal [MGO], glyoxal [GO] and 3-deoxyglucosone [3-DG]), and free AGEs (N(ε)-(carboxymethyl)lysine [CML], N(ε)-(carboxyethyl)lysine [CEL] and N(δ)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine [MG-H1]), and protein-bound AGEs (CML, CEL, pentosidine), as well as the complement activation products C3a and soluble C5b-9 (sC5b-9), were measured in 530 participants (59.5 ± 7.0 years [mean ± SD], 61% men) of the Cohort on Diabetes and Atherosclerosis Maastricht (CODAM) study. Multiple linear regression analyses were used to investigate the associations between dicarbonyl stress (standardised) and complement activation (standardised) with adjustment of potential confounders, including age, sex, lifestyle, use of medication and markers of obesity. In addition, the associations of two potentially functional polymorphisms (rs1049346, rs2736654) in the gene encoding glyoxalase 1 (GLO1), the rate-limiting detoxifying enzyme for MGO, with C3a and sC5b-9 (all standardized) were evaluated. RESULTS: After adjustment for potential confounders, plasma concentration of the dicarbonyl GO was inversely associated with sC5b-9 (β −0.12 [95% CI –0.21, −0.02]) and the protein-bound AGE CEL was inversely associated with C3a (−0.17 [−0.25, −0.08]). In contrast, the protein-bound AGE pentosidine was positively associated with sC5b-9 (0.15 [0.05, 0.24]). No associations were observed for other α-dicarbonyls and other free or protein-bound AGEs with C3a or sC5b-9. Individuals with the AG and AA genotype of rs1049346 had, on average, 0.32 and 0.40 SD lower plasma concentrations of sC5b-9 than those with the GG genotype, while concentrations of C3a did not differ significantly between rs1049346 genotypes. GLO1 rs2736654 was not associated with either C3a or sC5b-9. CONCLUSIONS/INTERPRETATION: Plasma concentrations of dicarbonyl stress markers showed distinct associations with complement activation products: some of them were inversely associated with either C3a or sC5b-9, while protein-bound pentosidine was consistently and positively associated with sC5b-9. This suggests different biological relationships of individual dicarbonyl stress markers with complement activation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00125-020-05098-4) contains peer-reviewed but unedited supplementary material, which is available to authorised users. Springer Berlin Heidelberg 2020-01-28 2020 /pmc/articles/PMC7145776/ /pubmed/31993713 http://dx.doi.org/10.1007/s00125-020-05098-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xin, Ying
Hertle, Elisabeth
van der Kallen, Carla J. H.
Schalkwijk, Casper G.
Stehouwer, Coen D. A.
van Greevenbroek, Marleen M. J.
Associations of dicarbonyl stress with complement activation: the CODAM study
title Associations of dicarbonyl stress with complement activation: the CODAM study
title_full Associations of dicarbonyl stress with complement activation: the CODAM study
title_fullStr Associations of dicarbonyl stress with complement activation: the CODAM study
title_full_unstemmed Associations of dicarbonyl stress with complement activation: the CODAM study
title_short Associations of dicarbonyl stress with complement activation: the CODAM study
title_sort associations of dicarbonyl stress with complement activation: the codam study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145776/
https://www.ncbi.nlm.nih.gov/pubmed/31993713
http://dx.doi.org/10.1007/s00125-020-05098-4
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