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Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model

Trans-resveratrol, a well-known plant phenolic compound, has been intensively investigated due to its association with the so-called French paradox. However, despite its high pharmacological potential, trans-resveratrol has shown relatively low bioavailability. Trans-resveratrol is intensively metab...

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Autores principales: Jarosova, Veronika, Vesely, Ondrej, Doskocil, Ivo, Tomisova, Katerina, Marsik, Petr, Jaimes, Jose D., Smejkal, Karel, Kloucek, Pavel, Havlik, Jaroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146108/
https://www.ncbi.nlm.nih.gov/pubmed/32106482
http://dx.doi.org/10.3390/nu12030595
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author Jarosova, Veronika
Vesely, Ondrej
Doskocil, Ivo
Tomisova, Katerina
Marsik, Petr
Jaimes, Jose D.
Smejkal, Karel
Kloucek, Pavel
Havlik, Jaroslav
author_facet Jarosova, Veronika
Vesely, Ondrej
Doskocil, Ivo
Tomisova, Katerina
Marsik, Petr
Jaimes, Jose D.
Smejkal, Karel
Kloucek, Pavel
Havlik, Jaroslav
author_sort Jarosova, Veronika
collection PubMed
description Trans-resveratrol, a well-known plant phenolic compound, has been intensively investigated due to its association with the so-called French paradox. However, despite its high pharmacological potential, trans-resveratrol has shown relatively low bioavailability. Trans-resveratrol is intensively metabolized in the intestine and liver, yielding metabolites that may be responsible for its high bioactivity. The aim of this study was to investigate and compare the metabolism of trans-resveratrol (tRes), cis-resveratrol (cRes) and dihydroresveratrol (dhRes) in an in vitro epithelial model using Caco-2 cell lines. Obtained metabolites of tRes, cRes and dhRes were analyzed by LC/MS Q-TOF, and significant differences in the metabolism of each compound were observed. The majority of tRes was transported unchanged through the Caco-2 cells, while cRes was mostly metabolized. The main metabolite of both cis- and trans-resveratrol observed as a result of colon microbial metabolism, dhRes, was metabolized almost completely, with only traces of the unchanged molecule being found. A sulphate conjugate was identified as the main metabolite of tRes in our model, while a glucuronide conjugate was the major metabolite of cRes and dhRes. Since metabolism of simple phenolics and polyphenols plays a crucial role in their bioavailability, detailed knowledge of their transformation is of high scientific value.
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spelling pubmed-71461082020-04-15 Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model Jarosova, Veronika Vesely, Ondrej Doskocil, Ivo Tomisova, Katerina Marsik, Petr Jaimes, Jose D. Smejkal, Karel Kloucek, Pavel Havlik, Jaroslav Nutrients Article Trans-resveratrol, a well-known plant phenolic compound, has been intensively investigated due to its association with the so-called French paradox. However, despite its high pharmacological potential, trans-resveratrol has shown relatively low bioavailability. Trans-resveratrol is intensively metabolized in the intestine and liver, yielding metabolites that may be responsible for its high bioactivity. The aim of this study was to investigate and compare the metabolism of trans-resveratrol (tRes), cis-resveratrol (cRes) and dihydroresveratrol (dhRes) in an in vitro epithelial model using Caco-2 cell lines. Obtained metabolites of tRes, cRes and dhRes were analyzed by LC/MS Q-TOF, and significant differences in the metabolism of each compound were observed. The majority of tRes was transported unchanged through the Caco-2 cells, while cRes was mostly metabolized. The main metabolite of both cis- and trans-resveratrol observed as a result of colon microbial metabolism, dhRes, was metabolized almost completely, with only traces of the unchanged molecule being found. A sulphate conjugate was identified as the main metabolite of tRes in our model, while a glucuronide conjugate was the major metabolite of cRes and dhRes. Since metabolism of simple phenolics and polyphenols plays a crucial role in their bioavailability, detailed knowledge of their transformation is of high scientific value. MDPI 2020-02-25 /pmc/articles/PMC7146108/ /pubmed/32106482 http://dx.doi.org/10.3390/nu12030595 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jarosova, Veronika
Vesely, Ondrej
Doskocil, Ivo
Tomisova, Katerina
Marsik, Petr
Jaimes, Jose D.
Smejkal, Karel
Kloucek, Pavel
Havlik, Jaroslav
Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model
title Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model
title_full Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model
title_fullStr Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model
title_full_unstemmed Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model
title_short Metabolism of cis- and trans-Resveratrol and Dihydroresveratrol in an Intestinal Epithelial Model
title_sort metabolism of cis- and trans-resveratrol and dihydroresveratrol in an intestinal epithelial model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146108/
https://www.ncbi.nlm.nih.gov/pubmed/32106482
http://dx.doi.org/10.3390/nu12030595
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