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Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of h...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146121/ https://www.ncbi.nlm.nih.gov/pubmed/32187981 http://dx.doi.org/10.3390/nu12030781 |
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author | Wallis, Katherine F. Melnyk, Stepan B. Miousse, Isabelle R. |
author_facet | Wallis, Katherine F. Melnyk, Stepan B. Miousse, Isabelle R. |
author_sort | Wallis, Katherine F. |
collection | PubMed |
description | Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families Bacteroidaceae and Verrucoccaceae (mostly A. mucinophila) and a decrease in Rumminococcaceae in animals fed a methionine-restricted diet compared to controls. In females, Bacteroidales family S24-7 was increased two-fold, while families Bacteroidaceae, Verrucoccaceae, Rumminococcaceae, and Rikenellaceae were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome. |
format | Online Article Text |
id | pubmed-7146121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71461212020-04-15 Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome Wallis, Katherine F. Melnyk, Stepan B. Miousse, Isabelle R. Nutrients Article Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families Bacteroidaceae and Verrucoccaceae (mostly A. mucinophila) and a decrease in Rumminococcaceae in animals fed a methionine-restricted diet compared to controls. In females, Bacteroidales family S24-7 was increased two-fold, while families Bacteroidaceae, Verrucoccaceae, Rumminococcaceae, and Rikenellaceae were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome. MDPI 2020-03-16 /pmc/articles/PMC7146121/ /pubmed/32187981 http://dx.doi.org/10.3390/nu12030781 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wallis, Katherine F. Melnyk, Stepan B. Miousse, Isabelle R. Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome |
title | Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome |
title_full | Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome |
title_fullStr | Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome |
title_full_unstemmed | Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome |
title_short | Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome |
title_sort | sex-specific effects of dietary methionine restriction on the intestinal microbiome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146121/ https://www.ncbi.nlm.nih.gov/pubmed/32187981 http://dx.doi.org/10.3390/nu12030781 |
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