Cargando…

Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome

Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of h...

Descripción completa

Detalles Bibliográficos
Autores principales: Wallis, Katherine F., Melnyk, Stepan B., Miousse, Isabelle R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146121/
https://www.ncbi.nlm.nih.gov/pubmed/32187981
http://dx.doi.org/10.3390/nu12030781
_version_ 1783520126592614400
author Wallis, Katherine F.
Melnyk, Stepan B.
Miousse, Isabelle R.
author_facet Wallis, Katherine F.
Melnyk, Stepan B.
Miousse, Isabelle R.
author_sort Wallis, Katherine F.
collection PubMed
description Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families Bacteroidaceae and Verrucoccaceae (mostly A. mucinophila) and a decrease in Rumminococcaceae in animals fed a methionine-restricted diet compared to controls. In females, Bacteroidales family S24-7 was increased two-fold, while families Bacteroidaceae, Verrucoccaceae, Rumminococcaceae, and Rikenellaceae were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome.
format Online
Article
Text
id pubmed-7146121
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71461212020-04-15 Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome Wallis, Katherine F. Melnyk, Stepan B. Miousse, Isabelle R. Nutrients Article Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families Bacteroidaceae and Verrucoccaceae (mostly A. mucinophila) and a decrease in Rumminococcaceae in animals fed a methionine-restricted diet compared to controls. In females, Bacteroidales family S24-7 was increased two-fold, while families Bacteroidaceae, Verrucoccaceae, Rumminococcaceae, and Rikenellaceae were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome. MDPI 2020-03-16 /pmc/articles/PMC7146121/ /pubmed/32187981 http://dx.doi.org/10.3390/nu12030781 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wallis, Katherine F.
Melnyk, Stepan B.
Miousse, Isabelle R.
Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
title Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
title_full Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
title_fullStr Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
title_full_unstemmed Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
title_short Sex-Specific Effects of Dietary Methionine Restriction on the Intestinal Microbiome
title_sort sex-specific effects of dietary methionine restriction on the intestinal microbiome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146121/
https://www.ncbi.nlm.nih.gov/pubmed/32187981
http://dx.doi.org/10.3390/nu12030781
work_keys_str_mv AT walliskatherinef sexspecificeffectsofdietarymethioninerestrictionontheintestinalmicrobiome
AT melnykstepanb sexspecificeffectsofdietarymethioninerestrictionontheintestinalmicrobiome
AT miousseisabeller sexspecificeffectsofdietarymethioninerestrictionontheintestinalmicrobiome