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Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice

A chronic high-fat diet (HFD) produces obesity, leading to pathological consequences in the liver and skeletal muscle. The fat in the liver leads to accumulation of a large number of intrahepatic lipid droplets (LD), which are susceptible to oxidation. Obesity also affects skeletal muscle, increasin...

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Autores principales: Pino-de la Fuente, Francisco, Nocetti, Diego, Sacristán, Camila, Ruiz, Paulina, Guerrero, Julia, Jorquera, Gonzalo, Uribe, Ernesto, Bucarey, José Luis, Espinosa, Alejandra, Puente, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146126/
https://www.ncbi.nlm.nih.gov/pubmed/32151028
http://dx.doi.org/10.3390/nu12030700
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author Pino-de la Fuente, Francisco
Nocetti, Diego
Sacristán, Camila
Ruiz, Paulina
Guerrero, Julia
Jorquera, Gonzalo
Uribe, Ernesto
Bucarey, José Luis
Espinosa, Alejandra
Puente, Luis
author_facet Pino-de la Fuente, Francisco
Nocetti, Diego
Sacristán, Camila
Ruiz, Paulina
Guerrero, Julia
Jorquera, Gonzalo
Uribe, Ernesto
Bucarey, José Luis
Espinosa, Alejandra
Puente, Luis
author_sort Pino-de la Fuente, Francisco
collection PubMed
description A chronic high-fat diet (HFD) produces obesity, leading to pathological consequences in the liver and skeletal muscle. The fat in the liver leads to accumulation of a large number of intrahepatic lipid droplets (LD), which are susceptible to oxidation. Obesity also affects skeletal muscle, increasing LD and producing insulin signaling impairment. Physalis peruviana L. (PP) (Solanaceae) is rich in peruvioses and has high antioxidant activity. We assessed the ability of PP to enhance insulin-dependent glucose uptake in skeletal muscle and the capacity to prevent both inflammation and lipoperoxidation in the liver of diet-induced obese mice. Male C57BL/6J mice were divided into groups and fed for eight weeks: control diet (C; 10% fat, 20% protein, 70% carbohydrates); C + PP (300 mg/kg/day); HFD (60% fat, 20% protein, 20% carbohydrates); and HFD + PP. Results suggest that PP reduces the intracellular lipoperoxidation level and the size of LD in both isolated hepatocytes and skeletal muscle fibers. PP also promotes insulin-dependent skeletal muscle glucose uptake. In conclusion, daily consumption of 300 mg/kg of fresh pulp of PP could be a novel strategy to prevent the hepatic lipoperoxidation and insulin resistance induced by obesity.
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spelling pubmed-71461262020-04-15 Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice Pino-de la Fuente, Francisco Nocetti, Diego Sacristán, Camila Ruiz, Paulina Guerrero, Julia Jorquera, Gonzalo Uribe, Ernesto Bucarey, José Luis Espinosa, Alejandra Puente, Luis Nutrients Article A chronic high-fat diet (HFD) produces obesity, leading to pathological consequences in the liver and skeletal muscle. The fat in the liver leads to accumulation of a large number of intrahepatic lipid droplets (LD), which are susceptible to oxidation. Obesity also affects skeletal muscle, increasing LD and producing insulin signaling impairment. Physalis peruviana L. (PP) (Solanaceae) is rich in peruvioses and has high antioxidant activity. We assessed the ability of PP to enhance insulin-dependent glucose uptake in skeletal muscle and the capacity to prevent both inflammation and lipoperoxidation in the liver of diet-induced obese mice. Male C57BL/6J mice were divided into groups and fed for eight weeks: control diet (C; 10% fat, 20% protein, 70% carbohydrates); C + PP (300 mg/kg/day); HFD (60% fat, 20% protein, 20% carbohydrates); and HFD + PP. Results suggest that PP reduces the intracellular lipoperoxidation level and the size of LD in both isolated hepatocytes and skeletal muscle fibers. PP also promotes insulin-dependent skeletal muscle glucose uptake. In conclusion, daily consumption of 300 mg/kg of fresh pulp of PP could be a novel strategy to prevent the hepatic lipoperoxidation and insulin resistance induced by obesity. MDPI 2020-03-05 /pmc/articles/PMC7146126/ /pubmed/32151028 http://dx.doi.org/10.3390/nu12030700 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pino-de la Fuente, Francisco
Nocetti, Diego
Sacristán, Camila
Ruiz, Paulina
Guerrero, Julia
Jorquera, Gonzalo
Uribe, Ernesto
Bucarey, José Luis
Espinosa, Alejandra
Puente, Luis
Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice
title Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice
title_full Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice
title_fullStr Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice
title_full_unstemmed Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice
title_short Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice
title_sort physalis peruviana l. pulp prevents liver inflammation and insulin resistance in skeletal muscles of diet-induced obese mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146126/
https://www.ncbi.nlm.nih.gov/pubmed/32151028
http://dx.doi.org/10.3390/nu12030700
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