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Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study

The objective of this pilot study was to assess the fecal microbiome and different immunological parameters in infant feces and maternal milk from mother–infant pairs in which the infants were suffering from different gastrointestinal disorders (colic, non-IgE-mediated cow milk protein allergy (CMPA...

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Autores principales: Aparicio, Marina, Alba, Claudio, Rodríguez, Juan Miguel, Fernández, Leonides
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146151/
https://www.ncbi.nlm.nih.gov/pubmed/32121004
http://dx.doi.org/10.3390/nu12030634
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author Aparicio, Marina
Alba, Claudio
Rodríguez, Juan Miguel
Fernández, Leonides
author_facet Aparicio, Marina
Alba, Claudio
Rodríguez, Juan Miguel
Fernández, Leonides
author_sort Aparicio, Marina
collection PubMed
description The objective of this pilot study was to assess the fecal microbiome and different immunological parameters in infant feces and maternal milk from mother–infant pairs in which the infants were suffering from different gastrointestinal disorders (colic, non-IgE-mediated cow milk protein allergy (CMPA), and proctocolitis). A cohort of 30 mother–infant pairs, in which the infants were diagnosed with these gastrointestinal disorders or included as healthy controls, were recruited. Bacterial composition of infant feces and breast milk was determined by metataxonomic sequencing. Immunological compounds were quantified using multiplexed immunoassays. A higher abundance of Eggerthellaceae, Lachnospiraceae and Peptostreptococcaceae, and lower abundance of Bifidobacterium and higher abundance of Rothia were registered in fecal samples from the CMPA group. Eggerthellaceae was also significantly more abundant in milk samples of the CMPA group. There were no differences in the concentration of immunological compounds in infant fecal samples between the four groups. In contrast, differences were found in the concentration and/or frequency of compounds related to acquired immunity and granulocyte colony stimulating factor (GCSF) in breast milk samples. In conclusion, a few microbial signatures in feces may explain part of the difference between CMPA and other infants. In addition, some milk immunological signatures have been uncovered among the different conditions addressed in this pilot study.
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spelling pubmed-71461512020-04-15 Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study Aparicio, Marina Alba, Claudio Rodríguez, Juan Miguel Fernández, Leonides Nutrients Article The objective of this pilot study was to assess the fecal microbiome and different immunological parameters in infant feces and maternal milk from mother–infant pairs in which the infants were suffering from different gastrointestinal disorders (colic, non-IgE-mediated cow milk protein allergy (CMPA), and proctocolitis). A cohort of 30 mother–infant pairs, in which the infants were diagnosed with these gastrointestinal disorders or included as healthy controls, were recruited. Bacterial composition of infant feces and breast milk was determined by metataxonomic sequencing. Immunological compounds were quantified using multiplexed immunoassays. A higher abundance of Eggerthellaceae, Lachnospiraceae and Peptostreptococcaceae, and lower abundance of Bifidobacterium and higher abundance of Rothia were registered in fecal samples from the CMPA group. Eggerthellaceae was also significantly more abundant in milk samples of the CMPA group. There were no differences in the concentration of immunological compounds in infant fecal samples between the four groups. In contrast, differences were found in the concentration and/or frequency of compounds related to acquired immunity and granulocyte colony stimulating factor (GCSF) in breast milk samples. In conclusion, a few microbial signatures in feces may explain part of the difference between CMPA and other infants. In addition, some milk immunological signatures have been uncovered among the different conditions addressed in this pilot study. MDPI 2020-02-27 /pmc/articles/PMC7146151/ /pubmed/32121004 http://dx.doi.org/10.3390/nu12030634 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aparicio, Marina
Alba, Claudio
Rodríguez, Juan Miguel
Fernández, Leonides
Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study
title Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study
title_full Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study
title_fullStr Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study
title_full_unstemmed Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study
title_short Microbiological and Immunological Markers in Milk and Infant Feces for Common Gastrointestinal Disorders: A Pilot Study
title_sort microbiological and immunological markers in milk and infant feces for common gastrointestinal disorders: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146151/
https://www.ncbi.nlm.nih.gov/pubmed/32121004
http://dx.doi.org/10.3390/nu12030634
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