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Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats

Acetaminophen (APAP) is one of the most used analgesics and antipyretic agents in the world. Intoxication with APAP is the main cause of acute liver toxicity in both the US and Europe. Spore-forming probiotic bacteria have the ability to resist harsh gastric and intestinal conditions. The aim of thi...

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Autores principales: Neag, Maria Adriana, Catinean, Adrian, Muntean, Dana Maria, Pop, Maria Raluca, Bocsan, Corina Ioana, Botan, Emil Claudiu, Buzoianu, Anca Dana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146158/
https://www.ncbi.nlm.nih.gov/pubmed/32120994
http://dx.doi.org/10.3390/nu12030632
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author Neag, Maria Adriana
Catinean, Adrian
Muntean, Dana Maria
Pop, Maria Raluca
Bocsan, Corina Ioana
Botan, Emil Claudiu
Buzoianu, Anca Dana
author_facet Neag, Maria Adriana
Catinean, Adrian
Muntean, Dana Maria
Pop, Maria Raluca
Bocsan, Corina Ioana
Botan, Emil Claudiu
Buzoianu, Anca Dana
author_sort Neag, Maria Adriana
collection PubMed
description Acetaminophen (APAP) is one of the most used analgesics and antipyretic agents in the world. Intoxication with APAP is the main cause of acute liver toxicity in both the US and Europe. Spore-forming probiotic bacteria have the ability to resist harsh gastric and intestinal conditions. The aim of this study was to investigate the possible protective effect of Bacillus (B) species (sp) spores (B. licheniformis, B. indicus, B. subtilis, B. clausii, B. coagulans) against hepatotoxicity induced by APAP in rats. A total of 35 rats were randomly divided into seven groups: group I served as control; group II received silymarin; group III received MegaSporeBiotic(TM) (MSB); group IV received APAP and served as the model of hepatotoxicity; group V received APAP and silymarin; group VI received APAP and MSB; group VII received APAP, silymarin and MSB. The livers for histopathological examination and blood samples were collected on the last day of the experiment. We determined aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total antioxidant capacity (TAC) levels and zonula occludens (ZO-1), tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) expression. APAP overdose increased AST and ALT. It slowly decreased TAC compared to the control group, but pretreatment with silymarin and MSB increased TAC levels. Elevated plasma concentrations were identified for ZO-1 in groups treated with APAP overdose compared with those without APAP or receiving APAP in combination with silymarin, MSB or both. The changes were positively correlated with the levels of other proinflammatory cytokines (TNF-α, IL-1β). In addition, histopathological hepatic injury was improved by preadministration of MSB or silymarin versus the disease model group. Bacillus sp spores had a protective effect on acute hepatic injury induced by APAP. Pretreatment with MSB resulted in a significant reduction in serum AST, ALT, TNF-α, IL-1β, ZO-1, TAC and also hepatocyte necrosis, similar to the well-known hepatoprotective agent—silymarin.
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spelling pubmed-71461582020-04-15 Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats Neag, Maria Adriana Catinean, Adrian Muntean, Dana Maria Pop, Maria Raluca Bocsan, Corina Ioana Botan, Emil Claudiu Buzoianu, Anca Dana Nutrients Article Acetaminophen (APAP) is one of the most used analgesics and antipyretic agents in the world. Intoxication with APAP is the main cause of acute liver toxicity in both the US and Europe. Spore-forming probiotic bacteria have the ability to resist harsh gastric and intestinal conditions. The aim of this study was to investigate the possible protective effect of Bacillus (B) species (sp) spores (B. licheniformis, B. indicus, B. subtilis, B. clausii, B. coagulans) against hepatotoxicity induced by APAP in rats. A total of 35 rats were randomly divided into seven groups: group I served as control; group II received silymarin; group III received MegaSporeBiotic(TM) (MSB); group IV received APAP and served as the model of hepatotoxicity; group V received APAP and silymarin; group VI received APAP and MSB; group VII received APAP, silymarin and MSB. The livers for histopathological examination and blood samples were collected on the last day of the experiment. We determined aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total antioxidant capacity (TAC) levels and zonula occludens (ZO-1), tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) expression. APAP overdose increased AST and ALT. It slowly decreased TAC compared to the control group, but pretreatment with silymarin and MSB increased TAC levels. Elevated plasma concentrations were identified for ZO-1 in groups treated with APAP overdose compared with those without APAP or receiving APAP in combination with silymarin, MSB or both. The changes were positively correlated with the levels of other proinflammatory cytokines (TNF-α, IL-1β). In addition, histopathological hepatic injury was improved by preadministration of MSB or silymarin versus the disease model group. Bacillus sp spores had a protective effect on acute hepatic injury induced by APAP. Pretreatment with MSB resulted in a significant reduction in serum AST, ALT, TNF-α, IL-1β, ZO-1, TAC and also hepatocyte necrosis, similar to the well-known hepatoprotective agent—silymarin. MDPI 2020-02-27 /pmc/articles/PMC7146158/ /pubmed/32120994 http://dx.doi.org/10.3390/nu12030632 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neag, Maria Adriana
Catinean, Adrian
Muntean, Dana Maria
Pop, Maria Raluca
Bocsan, Corina Ioana
Botan, Emil Claudiu
Buzoianu, Anca Dana
Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats
title Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats
title_full Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats
title_fullStr Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats
title_full_unstemmed Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats
title_short Probiotic Bacillus Spores Protect Against Acetaminophen Induced Acute Liver Injury in Rats
title_sort probiotic bacillus spores protect against acetaminophen induced acute liver injury in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146158/
https://www.ncbi.nlm.nih.gov/pubmed/32120994
http://dx.doi.org/10.3390/nu12030632
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