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Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146171/ https://www.ncbi.nlm.nih.gov/pubmed/32138172 http://dx.doi.org/10.3390/bios10030020 |
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author | Spagnolo, Sandro De La Franier, Brian Hianik, Tibor Thompson, Michael |
author_facet | Spagnolo, Sandro De La Franier, Brian Hianik, Tibor Thompson, Michael |
author_sort | Spagnolo, Sandro |
collection | PubMed |
description | This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties. |
format | Online Article Text |
id | pubmed-7146171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71461712020-04-15 Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology Spagnolo, Sandro De La Franier, Brian Hianik, Tibor Thompson, Michael Biosensors (Basel) Article This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties. MDPI 2020-03-03 /pmc/articles/PMC7146171/ /pubmed/32138172 http://dx.doi.org/10.3390/bios10030020 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Spagnolo, Sandro De La Franier, Brian Hianik, Tibor Thompson, Michael Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology |
title | Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology |
title_full | Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology |
title_fullStr | Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology |
title_full_unstemmed | Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology |
title_short | Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology |
title_sort | surface probe linker with tandem anti-fouling properties for application in biosensor technology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146171/ https://www.ncbi.nlm.nih.gov/pubmed/32138172 http://dx.doi.org/10.3390/bios10030020 |
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