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Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology

This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS)...

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Detalles Bibliográficos
Autores principales: Spagnolo, Sandro, De La Franier, Brian, Hianik, Tibor, Thompson, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146171/
https://www.ncbi.nlm.nih.gov/pubmed/32138172
http://dx.doi.org/10.3390/bios10030020
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author Spagnolo, Sandro
De La Franier, Brian
Hianik, Tibor
Thompson, Michael
author_facet Spagnolo, Sandro
De La Franier, Brian
Hianik, Tibor
Thompson, Michael
author_sort Spagnolo, Sandro
collection PubMed
description This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties.
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spelling pubmed-71461712020-04-15 Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology Spagnolo, Sandro De La Franier, Brian Hianik, Tibor Thompson, Michael Biosensors (Basel) Article This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties. MDPI 2020-03-03 /pmc/articles/PMC7146171/ /pubmed/32138172 http://dx.doi.org/10.3390/bios10030020 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spagnolo, Sandro
De La Franier, Brian
Hianik, Tibor
Thompson, Michael
Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
title Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
title_full Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
title_fullStr Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
title_full_unstemmed Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
title_short Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
title_sort surface probe linker with tandem anti-fouling properties for application in biosensor technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146171/
https://www.ncbi.nlm.nih.gov/pubmed/32138172
http://dx.doi.org/10.3390/bios10030020
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