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AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications

This paper presents a capacitive differential bridge structure with both AC and DC excitation and balancing capability for low cost electrode-solution interfacial capacitance biosensing applications. The proposed series RC balancing structure offers higher sensitivity, lower susceptibility to common...

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Detalles Bibliográficos
Autores principales: Neshani, Sara, Nyamekye, Charles K. A., Melvin, Scott, Smith, Emily A., Chen, Degang J., Neihart, Nathan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146243/
https://www.ncbi.nlm.nih.gov/pubmed/32235710
http://dx.doi.org/10.3390/bios10030028
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author Neshani, Sara
Nyamekye, Charles K. A.
Melvin, Scott
Smith, Emily A.
Chen, Degang J.
Neihart, Nathan M.
author_facet Neshani, Sara
Nyamekye, Charles K. A.
Melvin, Scott
Smith, Emily A.
Chen, Degang J.
Neihart, Nathan M.
author_sort Neshani, Sara
collection PubMed
description This paper presents a capacitive differential bridge structure with both AC and DC excitation and balancing capability for low cost electrode-solution interfacial capacitance biosensing applications. The proposed series RC balancing structure offers higher sensitivity, lower susceptibility to common-mode interferences, and drift control. To evaluate the bridge performance in practice, possible effects of initial bridge imbalance due to component mismatches are investigated considering the required resolution of the balancing networks, sensitivity, and linearity. This evaluation is also a guideline to designing the balancing networks, balancing algorithm and the proceeding readout interface circuitry. The proposed series RC bridge structure is implemented along with a custom single frequency real-time amplification/filtering readout board with real-time data acquisition and sine fitting. The main specifications for the implemented structure are 8-bit detection resolution if the total expected fractional capacitance change at the interface is roughly 1%. The characterization and measurement results show the effectiveness of the proposed structure in achieving the design target. The implemented structure successfully achieves distinct detection levels for tiny total capacitance change at the electrode-solution interface, utilizing Microcystin-(Leucine-Arginine) toxin dilutions as a proof of concept.
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spelling pubmed-71462432020-04-15 AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications Neshani, Sara Nyamekye, Charles K. A. Melvin, Scott Smith, Emily A. Chen, Degang J. Neihart, Nathan M. Biosensors (Basel) Article This paper presents a capacitive differential bridge structure with both AC and DC excitation and balancing capability for low cost electrode-solution interfacial capacitance biosensing applications. The proposed series RC balancing structure offers higher sensitivity, lower susceptibility to common-mode interferences, and drift control. To evaluate the bridge performance in practice, possible effects of initial bridge imbalance due to component mismatches are investigated considering the required resolution of the balancing networks, sensitivity, and linearity. This evaluation is also a guideline to designing the balancing networks, balancing algorithm and the proceeding readout interface circuitry. The proposed series RC bridge structure is implemented along with a custom single frequency real-time amplification/filtering readout board with real-time data acquisition and sine fitting. The main specifications for the implemented structure are 8-bit detection resolution if the total expected fractional capacitance change at the interface is roughly 1%. The characterization and measurement results show the effectiveness of the proposed structure in achieving the design target. The implemented structure successfully achieves distinct detection levels for tiny total capacitance change at the electrode-solution interface, utilizing Microcystin-(Leucine-Arginine) toxin dilutions as a proof of concept. MDPI 2020-03-22 /pmc/articles/PMC7146243/ /pubmed/32235710 http://dx.doi.org/10.3390/bios10030028 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neshani, Sara
Nyamekye, Charles K. A.
Melvin, Scott
Smith, Emily A.
Chen, Degang J.
Neihart, Nathan M.
AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications
title AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications
title_full AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications
title_fullStr AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications
title_full_unstemmed AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications
title_short AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications
title_sort ac and dc differential bridge structure suitable for electrochemical interfacial capacitance biosensing applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146243/
https://www.ncbi.nlm.nih.gov/pubmed/32235710
http://dx.doi.org/10.3390/bios10030028
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